ABSTRACT
<p><b>OBJECTIVE</b>To investigate clinicopathological features of DiGeorge syndrome (DGS).</p><p><b>METHOD</b>The clinical features, histological and immunohistochemical findings were analyzed in 5 cases of DGS by autopsy.</p><p><b>RESULTS</b>Five cases of DGS in male infants aged 4 days, 1 month, 7 months, 10 months, and 13 months respectively. Gross and microscopic observations revealed that thymic cortex was depleted of lymphocytes or showed few, dispersed lymphocytes. The thymic medulla showed predominantly epithelial cells with calcified Hassall bodies as well as lymphocyte depletion. T lymphocytes were also scarce in the tonsils, lymph nodes, spleen, and mucosa-associated lymphatic tissue of ileum. In addition, 3 of the 5 patients also showed parathyroid aplasia or dysplasia, and congenital hypertrophy of the ventricular septum.</p><p><b>CONCLUSIONS</b>The pathological changes indicate that clinicians should be aware of defects of immune system if the infants suffer from severe infections. Pathologists should recognize the importance of abnormalities of lymphohematopoietic tissues in the diagnosis of primary immunodeficiency diseases such as DGS.</p>
Subject(s)
Humans , Infant , Infant, Newborn , Male , Autopsy , DiGeorge Syndrome , Allergy and Immunology , Pathology , Virology , Hepatitis, Viral, Human , Pathology , Hypertrophy, Left Ventricular , Pathology , Lymphocyte Count , Parathyroid Glands , Pathology , Pneumonia, Viral , Pathology , T-Lymphocytes , Allergy and Immunology , Pathology , Thymus Gland , PathologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between the protein expression of T-lymphoma invasion and metastasis gene 1 (Tiam-1) and the biological behaviors of nasopharyngeal carcinoma (NPC).</p><p><b>METHODS</b>Immunohistochemistry was performed to detect the expressions of Tiam-1 protein in 60 specimens of NPC tissue, 20 specimens of chronic nasopharyngitis (CN) tissue, and 6 tumor tissues from nude mice inoculated with metastatic human NPC cells.</p><p><b>RESULTS</b>The positivity rate and average score for Tiam-1 expression were significantly higher in NPC tissues than in CN tissue (63.33% vs 36.67%, 2.9167 +/- 1.3057 vs 0.7000 +/- 0.9234; chi(2)=20.429, P=0.001; t=7.0162, P=0.0000, respectively). No difference was found in Tiam-1 expression among NPC patients in different T stages (F=2.36, P=0.0811), while the expression differed significantly between the patients with lymph node metastasis and those without metastasis, and also between patients with organ metastasis and those without (P=0.0001). High Tiam-1 expressions were found in the tumor tissues in nude mice inoculated with metastatic NPC cells.</p><p><b>CONCLUSION</b>Tiam-1 expression is closely associated with the invasiveness and metastasis of NPC, indicating that Tiam-1 is an important factor that promotes the invasion and metastasis of NPC.</p>
Subject(s)
Adult , Aged , Animals , Female , Humans , Male , Mice , Middle Aged , Guanine Nucleotide Exchange Factors , Genetics , Metabolism , Mice, Nude , Nasopharyngeal Neoplasms , Metabolism , Pathology , Neoplasm Invasiveness , Neoplasm Metastasis , Neoplasm Transplantation , T-Lymphoma Invasion and Metastasis-inducing Protein 1ABSTRACT
<p><b>OBJECTIVE</b>To explore biological aspects of Tiam1 gene expression in nasopharyngeal carcinoma cells.</p><p><b>METHODS</b>Tiam1/C1199HA expression plasmids were transfected into nasopharyngeal carcinoma cells of C666-1 and CNE1 by lipofectamine2000. RT-PCR, real-time PCR and Western blot Analyses were performed to evaluate the expression of Tiam1 mRNA and protein levels, respectively. In vitro cell adhesion, wound healing and matrigel invasion assays were used to study the biological impact of Tiam1 on cell adhesion, mobility and invasion.</p><p><b>RESULTS</b>Tiam1 over expression significantly increased the abilities of adhesion, migratory and invasion of C666-1 and CNE1 cells, comparing with that of the control untransfected cells (P < 0.05).</p><p><b>CONCLUSION</b>Tiam1 expression correlates with the invasion and metastasis of nasopharyngeal carcinoma cells.</p>
Subject(s)
Humans , Cell Adhesion , Cell Line, Tumor , Cell Movement , Gene Expression Regulation, Neoplastic , Guanine Nucleotide Exchange Factors , Genetics , Metabolism , Physiology , Nasopharyngeal Neoplasms , Metabolism , Pathology , Neoplasm Invasiveness , Neoplasm Metastasis , Plasmids , RNA, Messenger , Metabolism , T-Lymphoma Invasion and Metastasis-inducing Protein 1ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Tiam-l gene silencing on the metastasis of human colorectal carcinoma cell line SW480 in nude mice by real-time whole-body fluorescence imaging.</p><p><b>METHODS</b>Enhanced green fluorescence protein (EGFP)-labeled human colorectal carcinoma cells, SW480/EGFP(+)/Tiam-1(-) and SW480/EGFP(+), were implanted into nude mice via tail vein injection or orthotopic colonal inoculation, and real-time whole-body fluorescence imaging was performed to compare the difference in tumor progression and metastasis between the two cells.</p><p><b>RESULTS</b>Both SW480/EGFP(+) and SW480/ EGFP(+)/Tiam-1(-) cells stably expressed EGFP, and Tiam1 gene expression was reduced in SW480/EGFP(+)Tiam-1(-) to 30% of the expression level in SW480/EGFP(+) cells. The growth rate of the two cell lines had no significant difference in vitro (P>0.05), but SW480/EGFP(+)/Tiam1(-) cell proliferation and metastasis were depressed obviously in comparison with SW480/EGFP(+) in vivo (P<0.05).</p><p><b>CONCLUSION</b>Tiam-1 gene may play an important role in invasion and metastasis of human colorectal cancer.</p>