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Objective:To investigate the effect and possible mechanism of curcumin on the growth and invasion of thyroid carcinoma papillary K1 cells.Methods:K1 cells were randomly divided into control group, solvent control group, and curcumin group with 10, 30 and 50 μmol/L. The control group was only added with fresh medium, the solvent control group was added with dimethyl sulfoxide medium, and the 10, 30, 50 μmol/L curcumin groups were added with 10, 30 and 50 μmol/L curcumin respectively. The effects of curcumin on the growth of thyroid cancer papillary carcinoma K1 cells were observed by MTT assay. PI and Hochest3342 double staining were used to detect cell death. Transwell chamber was used to verify the effect on cell invasion. Western blot was used to observe the effects of curcumin on expression of Vimentin and E-cadherin.Results:Compared to the control group and solvent control group, the cell survival rate significantly decreased and the cell death rate of 10, 30, 50 μmol/L curcumin groups significantly increased ( P<0.05). The cell invasion rate (80.12% ± 3.43%, 65.59% ± 4.11%, 30.32% ± 4.67% vs. 100.00% ± 2.81%, 98.82% ± 2.18%) of 10, 30, 50 μmol/L curcumin groups significantly decreased ( P<0.05). The expression of E-cadherin (0.41 ± 0.12, 0.63 ± 0.14, 0.70 ± 0.13 vs. 0.34 ± 0.10, 0.35 ± 0.11) of 10, 30, 50 μmol/L curcumin groups significantly increased ( P<0.05), and the expression of Vimentin (0.70 ± 0.11, 0.22 ± 0.10 vs. 0.87 ± 0.12, 0.87 ± 0.13) of 10, 30, 50 μmol/L curcumin groups significantly decreased ( P<0.05). Conclusions:Curcumin can inhibit the proliferation and invasion of papillary thyroid carcinoma K1 cells and accelerate cell death.
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Objective To improve the skills of pharmacists in pharmaceutical care and to provide patients with better individualized pharmaceutical care.Methods A total of 785 traditional Chinese medicine (TCM) counseling outpatient cases, consultation modes, objectives, consulting drugs, consulting problems were analyzed.Results Office counseling was the dominant method for TCM counseling (85.10%), followed by telephone counseling (14.90%). Of all the counselings, patients and their relatives accounted for 80.63%, where females was 60.25% and males 39.75%; and patients over 40 years of age accounted for 81.91%. Of all the pharmacy counseling, heart system disorder were the major (14.79%), followed by drugs for Qi, blood and body fluid disorder (12.61%); the contents of counseling were diversity, which included dosage and administration amounts (29.04%), experience for drug use (14.14%), drug interactions (10.39%) and adverse reactions (9.55%). Conclusion TCM counseling could offer an effective platform of communication between pharmacists and patients, improve patients' medication compliance, and ensure patients' safe, rational, economical and effective.
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Objective To learn and analyze adverse reactions of compound salvia drop pills in order to promote rational use.Methods ADR reports of Compound Salvia Drop Pills were retrieve and analyze through CNKI database from 1979 to 2013.Results Ten ADR reports were found.Adverse reactions of compound salvia drop pills were mainly gastrointestinal reaction,including cardiovascular,uropoiesis,nervous reactions.Conclusion More attention should pay to the patient who have taken compound salvia drop pills.Preventive measures should be prepared for possible adverse reactions.Suitable treatment should be carried out promptly against severe ADR.
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The ADR reports of Chinese Caterpillar Fungus and its preparations were retrieved through CNKI database and further analyzed. It was found that their adverse actions were mainly allergic responses involved with urinary system, endocrine system, and gastrointestinal system simultaneously. Specification should be stipulated on the use of Chinese Caterpillar Fungus and its preparations.
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Objective To study the affection of cultivating regions to the quality of Radix Angelicae Sinensis and the scientific basis of authenticity of Radix Angelicae Sinensis.Methods The amounts of ferulic acid,ligustilide and total polysaccharide in Radix Angelicae Sinensis cultivated in Gansu,Yunnan, Sichuan,Shanxi provinces were determined by HPLC and UV spectrophotometry.Results Radix Angelicae Sinensis cultivated in Gansu province possesses the highest contents of ferulic acid,ligustilide and total polysaccharide among that from four regions.Conclusion The quality of Radix Angelicae Sinensis cultivated in Gansu province is better than that from other regions.It is reasonable to regard Radix Angelicae Sinensis cultivated in Gansu province as authentic medical herbs.