ABSTRACT
Seedling, seedling parts and callus cultures of onion were tested for their antidiabetic activity by feeding the tissue-extracts to diabetic rats. The results indicated much higher antidiabetic activity in callus cultures as compared to natural bulbs of onion. These results may be of pharmaceutical significance since the callus can be used as an alternative source for the isolation of antidiabetic compounds.
Subject(s)
Animals , Blood Glucose/analysis , Diabetes Mellitus, Experimental/chemically induced , Hypoglycemic Agents/pharmacology , Male , Onions/chemistry , Plant Proteins/pharmacology , Rats , Rats, WistarABSTRACT
The whole-body hyperthermia (40 degrees C, 1 hr) 20-48 hr prior to total-body irradiation (TBI) with 9 Gy gamma rays gave 80% protection as assessed by survival of the animals. However this was reduced to 50% when mice were irradiated 7 or 15 days after hyperthermia. The local hyperthermia (42 degrees C, 1 hr) given prior to irradiation, on the other hand, did not show any protective effect. The whole-body or local hyperthermia given after TBI had no protective effect on survival of animals.
Subject(s)
Animals , Gamma Rays , Hyperthermia, Induced , Male , Mice , Radiation Tolerance , Whole-Body IrradiationABSTRACT
The pathway of pentose synthesis in glucose-grown cells of Lactobacillus casei was ascertained. Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were present in glucose-grown cells, while transaldolase and transketolase were present only in traces. This suggested that only the oxidative arm of this pathway was operative in glucose-grown cells. On the other hand, in ribose-grown cells, transaldolase was induced with a concomitant suppression of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. These results were confirmed by the detection of labelled CO2 produced by L. casei grown on [1-14C]glucose. The activities of the enzymes of the oxidative pentose phosphate pathway as also the rate of CO2 formation were higher in the exponential phase of growth as compared to the stationary phase, when the requirement of the cells for pentoses for the formation of DNA and RNA was higher.
Subject(s)
Glucose/metabolism , Lacticaseibacillus casei/growth & development , Pentoses/biosynthesisABSTRACT
Glucose 6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) were separated and partially purified from glucose-grown cells of Lactobacillus casei. The enzymes had similar pH optima, thermosensitivity and molecular weights. They had different net charges and their pI values were 5.38 and 4.52, respectively. Histidine, arginine, lysine and cysteine residues were essential for the activity of G6PD, and all the above amino acids with the exception of lysine were required for 6PGD activity. Mg2+ activated 6PGD up to 15 mM concentration, above which it was inhibitory. It had no effect on G6PD activity. G6PD was specific for NADP+, but 6PGD showed some activity with NAD+ as the cofactor, although it was essentially NADP(+)-preferring. Both the enzymes, were inhibited by NADPH. 6PGD was also inhibited by its product, ribulose 5-phosphate. ATP inhibited 6PGD only at subsaturating concentrations of NADP+. The inhibition was sigmoidal in the absence of Mg2+ and hyperbolic in its presence.