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Purpose of the study: Nerve-muscle physiology is a very basic and vital module in undergraduate physiologycurriculum. The practical sessions on this system are demonstrated on amphibian (frog) nerve-musclepreparation, which becomes a limiting factor in most of the medical colleges of India. In this study, wepropose an alternative approach by using mammalian (rat) model for nerve-muscle physiology undergraduatepractical experiments.Materials and methods: Rat in-situ sciatic nerve-soleus muscle preparation was used to study neuromusculartransmission. Stimulation of nerve and recording of muscle contraction (force) were done by using digitalrecording system. To demonstrate fatigue, repetitive electrical stimulation was applied to nerve-musclepreparation and muscle twitches were recorded. A reduction in amplitude of contraction to 50% of their basalrecording was considered as onset of fatigue. To demonstrate site of fatigue, the muscle was stimulateddirectly after the fatigue was observed with nerve stimulation and muscle twitch recorded. To observe theeffect of neuromuscular blocking drug Pancuronium bromide, on neuromuscular transmission, in a separateset up the drug was injected in muscle belly at multiple sites and nerve was stimulated to elicit muscletwitch. The response (amplitude of muscle twitch) was compared with control (injection of 0.9% saline).Main findings: On repeated stimulation of nerve muscle preparation, initially there was an increase inamplitude of contraction but progressively amplitude went on decreasing. After development of fatigue ondirect muscle stimulation, amplitude recorded was same as the initial twitch amplitude. This demonstratesthat the site of fatigue is not the muscle. Further, as nerve is also non- fatigable, site of fatigue wasneuromuscular junction.Injection of Pancuronium bromide showed that the twitch amplitude decreased substantially than the control(saline injection) on stimulation of nerve, but on direct muscle stimulation amplitude of contraction remainedsame as initial, confirming the effect of drug on neuromuscular junction.
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Purpose of the study: The effect of free loaded and after loaded condition in mammalian skeletal musclecontraction can be studied using an in-situ nerve-muscle preparation in rat. The experimental setup mimicsthe physiological conditions very closely unlike the classical in vitro frog nerve-muscle preparation andhence could be considered as an effective practical module to demonstrate the work done in a skeletalmuscle in both free loaded and after loaded condition.Methodology: A simple, feasible and novel practical is designed in which muscle twitch is recorded in anin-situ nerve-muscle preparation of an anaesthetized rat under free loaded and after loaded condition atdifferent weights using an isotonic transducer. Work done was calculated under free loaded and after loadedcondition and compared.Main finding: Difference was observed with respect to displacement and work done during free loaded andafter loaded condition. Work done was higher in free loaded condition.Conclusion: We propose a simple and novel practical to demonstrate the effect of free loaded and afterloaded condition in muscle twitch for undergraduate teaching in physiology.
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Purpose of the study: Physiology practical complement learning of concepts taught in didactic theorylectures. Therefore, they are instrumental to comprehensive learning. Learning of physiology of excitabletissues is supplemented by observing the same phenomena in action in the laboratory. We developed anin-situ rat nerve-muscle model to demonstrate nerve-muscle physiology phenomenon to undergraduate andpostgraduate students. This mimics the physiological conditions unlike the Rana tigrina experiments whichwere done earlier. Further, frequent unavailability of frogs envisaged us to find an alternative for demonstrationof these concepts.Materials and methods: We developed and standardized an in-situ rat soleus muscle-sciatic nerve modelto demonstrate the phenomenon of nerve-muscle properties like simple muscle twitch, effect of strength ofstimulus, effect of two successive stimuli and tetanus to the undergraduate and postgraduate students atour institute.Results: Simple muscle twitch was recorded and effect of increasing strength of stimuli was observed.Thereafter, effect of two successive stimuli and genesis of tetanus was recorded in the same experimentalset up. We successfully standardised this in-situ model for teaching nerve and muscle physiology to our students.Conclusion: We propose an easy and effective experimental rat in-situ model for nerve-muscle physiologyteaching to students
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Background: A quarter of the world’s adult population has Metabolic Syndrome (MetS) and this condition is appearing with increasing frequency in children and adolescents due to the growing obesity epidemic within this young population. Objective: To determine the prevalence of metabolic syndrome (MetS) in the apparently healthy female medical students in BPSGMC for Women Khanpur Kalan, Sonepat and to find the risk factors associated with these conditions among the study subjects. Methods: This cross-sectional study was conducted in rural BPS Government Medical College for Women, Khanpur Kalan, Sonepat from April 2018 to September 2018 for a period of six months. The study population included all the undergraduate medical students of 2016 and 2017 batches. Semi-structured and pretested questionnaire was used to collect the data regarding demographic characteristics, anthropometric parameters and biochemical markers using standardized methods. MetS was defined as per National Cholesterol Education Programme Adult Treatment Panel (NCEP ATP III) criteria modified in 2004. Statistical analysis was carried out with R v 3.5.1 Results: The prevalence of metabolic syndrome was 9.8%. It was 7.7% in 17-19 years age group and 11.2% in 20-23 years age group. All the study subjects with MetS had abdominal obesity. MetS was significantly associated with elevated blood pressure, fasting plasma glucose ≥100 mg/dl, serum triglyceride ≥150 mg/dl and serum HDL cholesterol level <50 mg/dl. Conclusions: Metabolic syndrome exists in significant number among apparently healthy female medical students. Policy makers and healthcare providers can develop effective lifestyle interventions such as diet and physical activity for addressing the risk factors for metabolic syndrome.
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Many physiological hematological changes occur during pregnancy to accommodate the demands of the developing foetus. Infect, these changes may appear to be pathological in the non-pregnant state. Although physiological in nature, but abnormal hematological profile does affect pregnancy and its outcome. One of the most important underlying cause of maternal mortality is due to underlying hematological complications. This review deals with thorough understanding of range of these haematological parameters during pregnancy.
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Background & objectives: The relationship between obesity and self perception, particularly in children and young adults has important implications for physical and psychosocial health and well-being. A better understanding of this relationship could help target psychology services and public health strategies more effectively. The aim of the present study was to assess the effect of body mass index (BMI) on physical self concept and cognition of the first year medical undergraduate students in a medical college in north India. The relationship between physical self concept and academic performance and presence of any gender differences were also examined. Methods: The study was carried out on 18-21 yr old first year M.B.B.S. students of Maulana Azad Medical College, New Delhi, India. Physical self concept was assessed using short version of Physical Self Description Questionnaire (PSDQ-S) which is a psychometrically strong instrument for measuring multiple dimensions of physical self-concept. Cognition was assessed by P300 evoked potentials and academic performance was evaluated on the basis of marks obtained in anatomy, physiology and biochemistry subjects. Results: There was no association between BMI and physical self-concept or between BMI and cognition. Gender differences on physical self-concept were also insignificant. No correlation was seen between physical self-concept and academic performance. Interpretation & conclusion: The present results suggest that negative consequences of high body mass index on physical self-concept and cognition are not seen in young adults. It may be that academic achievement nullifies the effect on physical self-concept and the effect on cognition accumulates as the age progresses, therefore, appears later in life.
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Nine bacterial strains were isolated using xylan rich media. The bacterial strain KS09 was selected on the basis of qualitative and quantitative test. It was identified as Bacillus sp. via physiological, morphological and biochemical characterization. The xylanase was purified to homogeneity from crude extract of Bacillus sp. KS09 using ammonium sulphate fractioning and CM-Sephadex C-50. The final purification fold was 10.20 with a recovery of 36.18%. The enzyme was optimally active at 50°C, pH 7.0 and stable over a broad pH range of 6.0-11.0. The residual activity at 6.0-11.0 pH was 100% even upto 3 h of incubation. The enzyme showed 75, 70 and 60% thermal stability at 50, 55 and 60°C, respectively after 1 h of incubation. The kinetic parameters (Km 22.59 mM; Vmax 76.93 IU/mL) were estimated using Lineweaver-Burk plot for purified xylanase. The xylanase activity was inhibited by all the metal ions applied. The characteristic studies revealed that xylanase including its cellulase free nature, broad pH stability and temperature stability are particularly suited its industrial applications.
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BACKGROUND: Chronic myeloid leukemia (CML) is a clonal myeloproliferative expansion of primitive hematopoietic progenitor cells. MATERIALS AND METHODS: In the present study, CML samples were collected from various hospitals in Amritsar, Jalandhar and Ludhiana. RESULTS: Chromosomal alterations seen in peripheral blood lymphocytes of these treated and untreated cases of CML were satellite associations, double minutes, random loss, gain of C group chromosomes and presence of marker chromosome. No aberrations were observed in control samples. Karyotypic abnormalities have also been noted in the Ph-negative cells of some patients in disease remission. CONCLUSION: This is a novel phenomenon whose prognostic implications require thorough and systematic evaluation.
Subject(s)
Child , Female , Hematopoietic Stem Cells , Hospitals , Humans , India , Karyotyping/methods , Leukemia, Myeloid, Chronic-Phase/genetics , Male , Leukemia, Myeloid, Chronic-Phase/drug therapy , Leukemia, Myeloid, Chronic-Phase/therapy , Philadelphia Chromosome , Piperazines/therapeutic use , Pyrimidines/therapeutic useABSTRACT
BACKGROUND: Alterations in surface-active material may contribute to the pathogenesis of idiopathic pulmonary fibrosis both by increasing the elastic recoil due to surface forces and by promoting alveolar collapse. This study intends to evaluate the surfactant protein-A levels in bronchoalveolar lavage in patients of idiopathic pulmonary fibrosis. MATERIAL AND METHODS: Ten patients of idiopathic pulmonary fibrosis (IPF) of either sex were taken up for estimation of surfactant protein-A (SP-A) levels in bronchoalveolar lavage. The final diagnosis of IPF was based on histopathology and high resolution CT. Ten controls were taken out of which five patients were histopathologically proven cases of sarcoidosis and five patients with normal chest X-ray and CT scan. History, physical examination and routine investigations were done to rule out any concomitant illness. RESULTS: Age range of patients varied from 37-65 years (mean +/- SD of 50.65 +/- 9.05) and controls 30-62 years (42.50 +/- 9.95). Fiberoptic bronchoscopy and bronchoalveolar lavage was carried out and fluid aspirated was studied for surfactant protein-A. The level of surfactant protein-A recovered was lower in patients with idiopathic pulmonary fibrosis in comparison to control group p < 0.01 (1.86 +/- 1.26 and 5.76 +/- 2.0 microg/ml respectively). The levels of surfactant protein-A also revealed that the level decreased with derangement of pulmonary function. CONCLUSIONS: The study thus showed that the level of surfactant protein A is reduced in patients with idiopathic pulmonary fibrosis. It is likely that they have a significant role in pathogenesis of idiopathic pulmonary fibrosis and its progression. Surfactant-A may have important therapeutic implications. Further studies are required for a definite answer.