ABSTRACT
Objective:To study the effect of inflammatory factor and neurotransmitter of Yizhi Xingnao Fang combined with western medicine for patients with alzheimer's disease. Methods:124 cases alzheimer's patients from October 2014 to October 2016 were divided into observation group and control group,the control group given donepezil etc conventional western medicine therapy,ob-servation group given Yizhi Xingnao Fang combinde with western medicine therapy. 12 weeks after treatment,cerebrospinal fluid in-flammatory factors,neurotransmitter and clinical efficacy were compared between two groups. Results:Observation group effective rate 80.65% was significantly higher than control group 64.52% (X2= 4.052 9,P<0.05);cerebrospinal fluid TNF-α,IL-6 were significantly lower than control group,IL-4,IL-10 were significantly higher than control group (t=10.911,8.739,5.000,4.046,P<0.05,P<0.01);DA,5-HT,NE were significantly higher than control group(t=9.435,7.916,5.985,P<0.05,P<0.01). Conclusion:Yizhi Xingnao Fang combined with western medicine therapy help to improve the clinical symptoms and therapeutic effect,which may be related to the suppression of inflammatory symptoms,regulation of neurotransmitter expression level.
ABSTRACT
<p><b>BACKGROUND</b>The most significant biological change in intervertebral disc degeneration is the decrease of chondrocyte specific gene and protein expression of Sox9 and collagen type II. Interleukin-1 (IL-1) is not expressed in the normal intervertebral disc tissue but increases in the degenerated intervertebral disc tissue. This suggests that IL-1 may play a role in regulation of the expression of Sox9 and collagen type II.</p><p><b>METHODS</b>Human intervertebral disc cells were isolated and cultured. Sox9 and collagen type II expression during treatment with IL-1, with or without the nuclear factor-kappaB (NF-kappaB) activity inhibitor curcumin, were detected by using reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, and the activity of the NF-kappaB signaling pathway was detected by the electrophoretic mobility shift assay (EMSA).</p><p><b>RESULTS</b>IL-1 lowered the mRNA level and protein expression of Sox9 and collagen type II in the cultured intervertebral disc cells in a dose dependent manner (P < 0.05), and this effect was attenuated by curcumin. Curcumin alone had no effect on Sox9 and collagen type II expression (P > 0.05). IL-1 at concentrations of 0.1 ng/ml, 1 ng/ml and 10 ng/ml could stimulate the activity of NF-kappaB in the intervertebral disc cells in a dose dependent manner (P < 0.05) that was inhibited by curcumin.</p><p><b>CONCLUSIONS</b>We demonstrated the previously unknown function of IL-1 in inhibiting Sox9 and collagen type II via NF-kappaB in the intervertebral disc cells. This inhibition can be attenuated by curcumin, which is an effective NF-kappaB activity inhibitor.</p>