ABSTRACT
50 adult albino rats were divided randomly into 3 groups, i, e. partial hepatectomy group, sham-operation group and normal control group. Specimens of tail portion of pancreas were taken at 1, 2, 3, 6 and 14 days after operation respectively. Paraffin sections were made and stained with immunohistochemical PAP method to demonstrate insulin-containing B cells,C-peptide-containing B cells,glucagon-containing A cells and somatostatin-containing .D cells. The changes in hormone secretion activity of B, A and D cells have been observed during liver regeneration period. Based on the different amount of immunoreactive product, the endocrine activity of islet cells can be divided into 3 grades: +, ++, and +++. Percentage of each grade of islet cells was calculated and taken as the parameter of endocrine activity of islet cells. Insulin release activity of B cells increased after 1-3 days of operation, and insulin biosynthesis and/or storage enhanced 3-6 days after. Glucagon release activity of A cells raised gradually during 1-3 days after operation, and somatostatin released activity of D cells declined at the same time. After 6 days, endocrine activity of A cells returned to normal level, but release of somatostatin of D cells elevated. Endocrine activity of islet cells all recovered to normal level 14 days later. The changes of endocrine activity of B cells and A cells were closely related to and parallel with regeneration process of liver. The changes of D cells suggest that it probably be a reflection of regulation action on endocrine activities of B cells and A cells.
ABSTRACT
50 adult rats divided into 3 groups, i. e. partial hepatectomy, sham-operation and normal control groups. The submandibular glands (SMG) were removed at different intervals (1-14 days) after operation, and stained with histological and histochemical method. On the 1-3 days after operation, especially on 3 days, some changes were found in granular convoluted tubule (GCT) cells of SMG. The secretory granules in the GCT cells were decreased in number. The reactions of PAS, -SS- group, PTAH were weaker than those of the sham-operation group, but the reactions of content in tubule system of SMG were raised. The reaction of SDH was stronger than those of the sham-operation group and the activities of NE and AcP decreased. After 7 days all the changes gradually restored, except the activities of ASDH increased and NE decreased continuously. After 14 days, all the changes became nearly normal. In addition, the reactions of RNA, G6Pase and 5-Nase in GCT cells of hepatectomized rats did not show apparent changes. The results suggested that, the changes of SMG GCT cells following partial hepatectomy seem, to be closely related with regenerative process of liver.
ABSTRACT
Fifty-one male rats (body weights:200—350gm) were raised with laboratory diet, 39 of them were injected once a week with CCl_4 (0.015cc/100gm body weight) in li- quid paraffin through the mesenteric veins and 12 rats without treatment as controls. Rats with 1,2,3,and 4 injections were killed 7 days afterwards.Pieces of liver tissue were taken from the left anterior lobe of the rats from both experimental and control groups.Paraffin sections were prepared from materials fixed in:(1)Susa or Stieve's for Heidenhain's Azan staining;(2)Regaud's for Bensley's acid fuchsin-methyl green staining of mitochondria;(3)cold Carnoy's for methyl green-pyronin staining of ribo- nucleic acid (RNA),with control sections extracted with 20% perchloric acid or 0.17 M NaCl at 56℃ for 2 hrs.;(4)Carnoy's for Yakovlev's ((?)) and Barrnett- Seligman's methods for protein-bound SH-group,with control sections blocked with 0.1M monoiodoacetic acid;(5)Formol-alcohol for Hotchkiss's periodic acid-Schiff's (PAS) reaction of glycogen,with control sections treated with salivary digestion;(6)cold ace- tone for alkaline phosphatase (ALP);and (7) 10% neutral formalin for frozen sections and Sudan Ⅲ testing for fats. After CCl_4-intoxication,during the development of cirrhosis,most of the parenchymal cells degenerated and finally became necrotic.Free fat droplets and hyperplastic fibrous tissues accumulated in the necrotic regions.The liver lobules thus were destroyed and blood sinuses were engorged with blood cells.Surviving cells showed compensative hyperplasia and were isolated into nodules by the developing fibrous tissues.Cirrhosis developed in all the rats treated with CCl_4. The parenchymal cells showed a progressive changes during the development of cirrhosis.There was an increase of fat droplets and a decrease of the number of mito- chondria in the cytoplasm.The mitochondria fragmented into granules or fused in a mass.There was a progressive decline in the content of RNA and glycogen,but an increase of the ALP activity.Those cells showing compensative hyperplasia enlarged in size,and their number of mitochondria,RNA and glycogen content progressively in- creased. Small-sized cells appeared in the necrotic regions among the fat droplets,necrotic cells and fibrous tissues.They decreased in number as the fibrous tissue developed. These cells contained mitochondria and showed positive reactions of PAS,RNA and ALP.Their origin was discussed and their relation to the formation of collagen fibers needs further investigation.