ABSTRACT
In this study, the effects of antibacterial activity of Urtica dioica and Iris pseudacorus essential oils, native plant northern of Iran, were investigated for some selected bacteria. The influence of essential oils was tested by the using of disk diffusion and micro-broth dilution methods against standard strains of the picked out bacteria. Gas Chromatography/Mass Spectroscopy [GC/MS] analysis, bioactivity determination, Minimum inhibitory concentrations [MIC] and minimum bactericidal concentration [MBC] of essential oils were utilized for this goal. This study showed that, Inhibition zone diameter varied from 11 to 19 mm and 9 to 17 mm for Urtica dioica and Iris pseudacorus respectively. In contrast, this figure fluctuated from 19 to 28 mm and 7 to 17 mm for gentamicin and ampicillin separately. By the application of micro-broth dilution technique, MICs for 1% essential oils were 1.8-7.5 micro g/ml and 3.75-15 micro g/ml for, Urtica dioica and Iris pseudacorus against gram-positive and gram-negative bacteria individually. Furthermore, the MBCs of herbal essences were 1.8-15 micro g/ml for, Urtica dioica and 15-30 micro g/ml for Iris. The application of essential oils for the bio-control of diseases, as a novel emerging alternative to antimicrobial treatments, lead to safer and more environmental management for infective diseases
ABSTRACT
The purpose of this study was to determine antibacterial activity of essential oil of Satureja khuzestanica against Listeria monocytogenes [PTCC1295] and strains isolated from breast milk show that. In this descriptive-analytic study, Essence of leave's plant was extracted and identified its compounds and then carvacrol was isolated. Antibacterial activities were examined by agar dilution method against L. monocytogenes. Minimum inhibitory concentration [MIC] and minimum bacterial concentration [MBC] were carried out by micro dilution method. Then bacterial suspension injected the BALB/c mice. Forty-eight h after seeing the listeriosis disease signs were started the treatment. Ampicillin [10 micro g/disc] and trimethoprim [5 g] were used as controls. The results showed that the inhibitory zone diameter standard and essential oils for strains isolated species were respectively 59 and 50 mm. This amount was determined by carvacrol, respectively, 60 and 48 mm. Inhibition zone diameter measurements for standard strains of ampicillin and trimethoprim tedious strains, respectively, 21, 40, 18 and 33 mm, respectively. The minimum inhibitory concentration of essential oils, carvacrol and ampicillin than standard strains, respectively 1.56, 1.56 and 155x10[-8] micro g/mL and MBC 3.125, 3.125 and 125x10[-7] micro g/mL was determined by the ratio of the strain 3.125, 3.125 and 0.0062 micro g/mL and MBC was 6.25, 6.25 and 0.025 micro g/mL. This study showed that bacterial cleansing properties of essential oil of this plant have a strong and effective combination that is carvacrol
ABSTRACT
Many of the plants used to treat certain diseases, because they have showed antimicrobial activity. In this case, many studies have conducted on antimicrobial and antioxidant activity of Artemisia annua. The purpose of this study is to determine the antibacterial effects of aqueous, chloroform, methanol and ethanol extracts of A. annua against eight bacterial species. Antimicrobial activity, minimum inhibitory concentration and minimum bactericidal activity of the essential oil and extract was performed by agar disc diffusion and microdilution broth methods. The obtained results showed antibacterial activity of the organic and chloroformic extracts of Artemisia annua against the tested microorganisms. Presence of tannins, saponins, alkaloids, amino acids, phenolic compounds, quinines and terpenoids were identified in the composition of the obtained extract using mass gas-chromatograph. The best result for the minimum inhibitory Concentration and minimum bactericidal concentration was reported for the 32 mg/ml of chloroformic extract. The results indicate the fact that the extracts and essential oils of the plants can be useful as medicinal or preservatives composition
ABSTRACT
Anagalis is the name of Scarlet Pimpernel, with about 20 to 25 species of flowering plants in the family Myrsinaceae. The common variety [A. arvensis] is a low-growing annual plant found in Europe, Asia and North America. In this Investigation, the inhibitory effects of methanol extract of Anagalis arvensis leaves and drug nystatin on clinical isolates and standard strain of Candida albicans [14053 PTCC] isolated from patients with vulvovaginal candidiasis were studied. In the laboratory study of 45 clinical isolates of Candida albicans was used and inhibitory effects of the extract were evaluated using by serial dilution Method. Chemical compounds of A. arvensis extract including alkaloids, glycosides, phenolic compounds, Terpenoids and amino acids were identified. The results showed that the minimum inhibitory concentration [MIC] nystatin against clinical isolates and standard strain of Candida albicans were similar and drug nystatin revealed the better MIC against standard strain and clinical isolates of C. albicans. Methanol extract of A. arvensis were inhibitory on standard strain and clinical isolates of C. albicans. The minimum inhibitory concentration of extract in comparison with the drug nystatin was less while the composition of the growth inhibitory concentration was greater than drug solely. This study indicated that the antimicrobial activity of this plant extract was considerable compared to drug nystatin and it demonstrate a basis for further research to isolate the active components in a variety of microbial strains