ABSTRACT
Expression of rbcS genes encoding small subunit of rubisco, most abundant protein in green tissue, is regulated by at least three parameters--tissue type, light conditions and stage of development. One of the green tissue-specific promoters of rbcS gene family was isolated from pigeonpea by PCR. Expression of uidA gene encoding beta-glucuronidase in the transgenic tobacco plants under the control of pigeonpea rbcS promoter, clearly showed that this promoter was as strong as pea rbcS3A promoter characterized earlier. Study of the sequence similarity with pea rbcS3A promoter, especially the region (boxes I and III) that is required for rbcS3A expression, showed more than 50% divergence. In contrast, pigeonpea promoter sequence isolated in the present study was more similar to that of spinach and rice rbcS promoters.
Subject(s)
Base Sequence , Cajanus/genetics , DNA, Plant/genetics , Genes, Plant/genetics , Molecular Sequence Data , Plant Leaves/genetics , Plants, Genetically Modified , Promoter Regions, GeneticABSTRACT
RAPD based fingerprinting of 21 serovars of Bacillus thuringiensis (Bt) representing different serotypes was performed using 19 random decamer primers. A total of 172 polymorphic fragments, ranging in size from 161-2789 bp, were amplified from 13 of the 19 primers. Pairwise genetic similarity analysis revealed very low similarity values, ranging from 3-68%, among the serovars of Bt, indicating high genetic divergence. Nineteen serovars of Bt fell in two major clusters and remaining two formed solitary clusters in the dendogram. Clustering of Bt strains established genetic relatedness between serovars and serotypes. It has been suggested that RAPD analysis can be used for genotypic characterization of Bt to complement flagellar serotyping.
Subject(s)
Bacillus thuringiensis/classification , Cluster Analysis , DNA Fingerprinting , DNA Primers/chemistry , DNA, Bacterial/genetics , Genetic Variation , Phylogeny , Polymerase Chain Reaction , Polymorphism, Genetic , Random Amplified Polymorphic DNA Technique , SerotypingABSTRACT
Effects of methionine sulphoximine (MSX) on glutamine synthetase activity and ammonia accumulation in the leaves of barley, sorghum and Moricandia arvensis were studied. Glutamine synthetase activity of sorghum leaves was relatively resistant to MSX treatment during initial stages. All the three plant species exhibited considerable accumulation of ammonia arising from non-photorespiratory sources, in response to MSX treatment. Minimal estimates of the rate of glycine decarboxylation were made employing MSX and isonicotinyl hydrazide together in the treatment solution. Changes in the pool sizes of amino acids involved in photorespiratory nitrogen metabolism as affected by MSX have also been investigated.