ABSTRACT
Introduction: Neoplasms that are derived from odontogenic tissues represent an uncommon and heterogenous group of enti- ties in oral and maxillofacial region comprising about 1% of all jaw tumors. Ameloblastoma received particular attention of oral pathologists due to its local aggressive behavior. Objective: Indian studies, regarding prevalence of ameloblastoma are very few. The present study was done to analyze and compare clinical, radiographic and histopathologic features of 57 cases of ameloblastomas in India retrospectively. Materials and methods: 57 cases of histologically diagnosed ameloblastomas were retrieved from the archives over a five year period to analyze the age, sex, site, clinical presentation, radiographic features and histopathology. Results: In the present study, ameloblastoma accounted for 77% of all odontogenic tumors with a mean age of 37.5 years in males and 42 years in females. About 60% of the cases revealed mandible as most common site predominantly of posterior re- gion with common clinical presentation as painless swelling. Radiographically, multilocular radiolucency was noticed in about 54.3% of cases. Among the histological variants of solid/multicystic ameloblastomas, follicular variant was reported to be the most common (38%) and intraluminal variant (19%) to be the common subtype in unicystic ameloblastomas. Statistical analy- sis was done among the histological variants of ameloblastomas which showed no statistical significance (p<0.05). Conclusion: The findings in the present study were well in accordance with previous reported studies. However, extensive studies with larger samples need to be carried out in the Indian population to evaluate the incidence of ameloblastoma for more definitive results.
ABSTRACT
Abstract we report A. rhizogenes-induced hairy root formation in S. bryopteris, a medicinally and commercially important plant. A. rhizogenes strain LBA1334 co-cultivated with explants (root, rhizophore, stem portion near the root, and stem with intact fronds) for 24 and 48 h after transformation for induction of hairy roots. The induction of hairy root was observed after 6 days of infection in case of 48 h co-cultivation only. PCR with rolA and virC gene specific primers confirmed the induced hairy roots were due to Ri T-DNA integration and not due to contaminating A. rhizogenes. The root network as explants showed the maximum transformation efficiency. We tested different media like MS, SHFR (Stage Hog Fern Root) and KNOP's during transformation for hairy root induction. The SHFR based media showed good response in transformation as well as propagation. Further, transformation efficiency was enhanced by addition of TDZ (2 mg/L) and Bevistin (0.1%) in SHFR media. The present work would be helpful in hairy roots-based in vitro production of secondary metabolites and on aspect of functional genomics of S. bryopteris.
Subject(s)
Transformation, Genetic/genetics , Polymerase Chain Reaction , Selaginellaceae/microbiology , Agrobacterium/genetics , GenomicsABSTRACT
Background: Non-Alcoholic fatty liver disease (NAFLD) has become a worldwide health concern with increase in the global incidence of obesity and it is now considered the hepatic component of the metabolic syndrome. Aims and Objective: The study’s aim was to compare the indices of the Liver Function tests in compensated chronic liver disease patients with and without NAFLD. Materials and Methods: A total of 100 consecutive patients with compensated chronic liver disease were recruited into the study. A structured questionnaire was administered to obtain relevant socio-demographic data. NAFLD was diagnosed based on clinical, biochemical, ultrasonographic and in a few histological features. The Adult Treatment Panel III criteria were used to identify patients with the metabolic syndrome. Results: In our study, 100 participants were recruited into study. 40 out of 60 patients (67%) showed grade-1 fatty liver findings and 20 out of 60 patients (33%) showed grade-2 fatty liver. Approximately 19% had fatty liver finding. The mean (SD) age of persons with NAFLD was 45.12 (±8.07) years compared to 47.49(±11.79) years for persons without NAFLD. The difference was not statistically significant (p=0.2). Body mass index (BMI), central obesity (waist circumference), fasting blood sugar, blood pressure, total cholesterol and triglycerides were significantly higher in the NAFLD group (p= <0.05) respectively. Conclusion: Indices of the deranged Liver functions were more prevalent in persons with NAFLD. It is recommended that patients with NAFLD be screened for metabolic syndrome and appropriate therapy instituted to decrease the risk of both hepatic and cardiovascular complications.
ABSTRACT
Sarcoidosis is a multisystem disease of obscure etiology that may involve almost any organ system; therefore, it results in various clinical manifestations. We are presenting a case of 60-year-old female complaining of multiple raised skin colored, to reddish lesions over face and few lesions over neck and extremities since 4 months cutaneous examination revealed multiple well defined skin colored to erythematous papulo-nodular lesions involving whole face with few discrete papular lesions over neck and extremities. Nasal skin was globular, studded with multiple nodules suggestive of lupus pernio. Histopathology showed many non-caseating naked epithelioid cell granulomas of variable sizes in the dermis. He was given oral methylprednisolone 16 mg twice daily after meal along with oral methotrexate 15 mg once in a week and tablet hydroxychloroquine 200 mg twice daily with keeping an eye over related adverse effects of all prescribed drugs. Still patient did not get remission after 6 months of a complete therapy. Due to the rarity of this disorder and its non-responding nature and course with conventional modalities of treatment made us to report this article for further studies and research.