Molecular study on foot and mouth disease virus in Beheira governorate, Egypt during 2014

Foot and mouth disease [FMD] is the most contagious disease of mammals and has a great potential for causing severe economic loss in susceptible cloven-hoofed animals. Egypt has a long history of occurrence of FMDV outbreaks, as the country is dependent on importion of live animals and meat from many countries all over the world. The present study was designed for detection, isolation and molecular characterization of FMDV circulationg among different regions in Beheira governorate. Thirty-eight tissue samples were collected from clinically diseased cattle and buffalo from different localities of Beheira governorate. Direct detection of FMDV using ELISA revealed that 84.2% of the samples were possitive. Molecular characterization showed that 24 samples [75%] were possitive for serotype O and eight samples [25%] were positive for serotype SAT2. This indicates the predominance of serotype O FMDV in Beheira, Egypt

Studies on bovine field skin lesions in Fayoum governorate

In this study an outbreak in Fayoum Governorate during summer 1998 showed skin lesions, fever [40-41°C] which resembled clinical manifestation of lumpy skin disease [LSD]. The skin lesion were in the form of firm rounded skin nodules which were covered with erected hair. 15 cases among cattle herd have been recorded, samples from all suspected diseased animals were taken. The samples were inoculated on chorioallantoic membrane [CAM] of emberyonated chicken egg [ECE] aged 9-11 days, for several passages and in MDBK tissue culture for isolation. Confirmatory tests were carried out by using standard specific antisera against LSD virus using agar gel immunodiffusion test AGID, immunoperoxidase and immunofluorescent. The positive lesions of CAM [pock lesion] were collected on 10% neutral formalin buffer for istopathological examination

Dietary folate intake, serum folate concentration and knowledge about folate in women of childbearing age

This study was conducted on 30 young women [19-23 years] in order to investigate dietary folate intake, serum concentrations of folate and knowledge about it.The associations of folate intake and serum concentration of folate with other factors such as socioeconomic, anthropometric and dietary factors were studied also. Dietary intakes of energy and selected nutrients were estimated using 24-hour recall for seven days. Nutritive value of foods was calculated and was evaluated using recommended nutrient intakes [RNI]. Body weight and height were measured and body mass index [BMI] was calculated.Each participant was asked to fill out questionnaire that include questions which assess her knowledge about folate. Blood samples were collected for the determination serum folate, hemoglobin and hematocrit concentrations and red blood cell folate concentrations were calculated. Results revealed that mean hemoglobin and hematocrit values of the sample were [12.95 +/- 0.96 g/dl and 36.12 +/- 1.83%] respectively. Mean serum folate was [4.30 +/- 0.43 ng/ml] while mean RBC folate was [262.11 +/- 21.52 ng/ml].All subjects have values within the reference range. Data showed that mean intake of folate was [240.93 +/- 100.37 micro g/day] and the mean consumption of both iron and folate covers only about [60%] of RNI. The differences in serum, RBC and dietary folate according to economic classes were not significant, while the differences between the four BMI categories concerning serum and RBC folate were highly significant. In addition, folic acid knowledge did not have any influence on serum, RBC folate or dietary folate intake. Serum folate showed significant positive correlations with dietary folate, protein, fiber, all vitamin B complex group except vitamin B6. Moreover, dietary folate had also significant positive correlation with serum folate, energy, protein, fiber, iron, the majority of vitamin B complex group and vitamin C. It could concluded that the nutritional status of folate among the present sample is fairly acceptable. So, it would be appropriate to implement strategies to increase the consumption of folate rich food among this group and to support the need for folate fortification of certain stable foods in order to secure sufficient intake of folate in the whole population