Protective effect and mechanism of Maiwei Yangfei Decoction on pulmonary fibrosis mice based on Nrf2 regulation of oxidative stress / 中国中药杂志

This study explored the effect and mechanism of Maiwei Yangfei Decoction(MWYF) on pulmonary fibrosis(PF) mice. MWYF was prepared, and its main components were detected by ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS). Male C57BL/6J mice were randomly divided into a control group, a model group, a pirfenidone(PFD) group, and low-, medium-, and high-dose MWYF groups, with 10 mice in each group. The PF model was induced in mice except for those in the control group by intratracheal instillation of bleomycin(BLM), and model mice were treated with saline or MWYF or PFD by gavage the next day. The water consumption, food intake, hair, and activity of mice were observed daily. The pathological changes in lung tissues were observed by hematoxylin-eosin(HE) staining, Masson staining, and CT scanning. The level of hydroxyproline(HYP) in lung tissues was detected by alkaline hydrolysis. Immunohistochemistry was used to observe the expression of collagen type Ⅲ(COL3) and fibronectin. The mRNA expression levels of α-smooth muscle actin(α-SMA), type Ⅰ collagen α1(COL1α1), COL3, and vimentin were detected by reverse transcription real-time fluorescence quantitative polymerase chain reaction(RT-qPCR). Superoxide dismutase(SOD) and malondialdehyde(MDA) kits were used to detect oxidative stress indicators in lung tissues and serum. The nuclear translocation of nuclear factor E2-related factor 2(Nrf2) protein was detected by immunofluorescence. The protein and mRNA expression levels of Nrf2, catalase(CAT), and heme oxygenase 1(HO-1) in lung tissues were detected by Western blot and RT-qPCR. Twelve chemical components were detected by UPLC-MS/MS. Animal experiments showed that MWYF could improve alveolar inflammation, collagen deposition, and fibrosis in PF mice, increase body weight of mice, and down-regulate the expression of fibrosis indexes such as HYP, α-SMA, COL1α1, COL3, fibronectin, and vimentin in lung tissues. In addition, MWYF could potentiate the activity of SOD in lung tissues and serum of PF mice, up-regulate the expression level of Nrf2, and promote its transfer to the nucleus, up-regulate the levels of downstream antioxidant target genes CAT and HO-1, and then reduce the accumulation of lipid metabolite MDA. In summary, MWYF can significantly improve the pathological damage and fibrosis of lung tissues in PF mice, and its mechanism may be related to the activation of the Nrf2 pathway to regulate oxidative stress.

Effects of health behavior intervention strategies based on health action process approach model on medication compliance of asthma patients / 中国实用护理杂志

Objective:To explore the influence of health behavior intervention strategies based on health action process approach model on medication compliance of asthma patients.Methods:Selected in July 2018 to March 2019 in the respiratory medicine clinics, 405 cases of patients with asthma as the research object, 205 cases were randomly divided into observation group and control group 200 cases, control group to implement regular medication guidance and health education, group implementing general guidelines and based on the health action process approach model building model of health education. The compliance of asthma patients before and after intervention was compared between the observation group and the control group .Results:After 3 months intervention, the behavior change stage of the observation group was significantly advanced ( Z value was -5.999, P<0.01), among which the proportion of patients in the action stage was the highest 58.7% (98/167). After intervention 1 month and 3 months, the compliance scores of patients of the observation group were 40.34±4.20 and 44.05±3.49, and those in the control group were 33.25±5.05 and 34.89±4.19. The differences between the two groups were statistically significant ( t values were 13.895, 21.646, P<0.01). Conclusion:The model of health education for asthma patients based on health action process approach model is helpful to improve the medication compliance of patients, and has a positive guiding significance for the construction of healthy lifestyle of asthma patients.

In vitro antibacterial activity analysis of antibacterial peptide lycosin-Ⅰ against clinical isolates of Pseudomonas aeruginosa / 临床检验杂志

Objective To investigate the in vitro antibacterial activity of lycosin-Ⅰ against clinical isolates of Pseudomonas aeruginosa.Methods The clinical isolates of Pseudomonas aeruginosa,including 10 strains of multidrug resistant Pseudomonas aeruginosa and 10 strains of non-multidrug resistant Pseudomonas aeruginosa,were randomly collected from the Second Xiangya Hospital of Central South University.The in vitro minimum inhibitory concentrations (MIC) of lycosin-Ⅰ against Pseudomonas aeruginosa were detected by the broth microdilution method.The representative isolates of multidrug resistant (Isolate 8,MIC =8 μg/mL) and non-multidrug resistant (Isolate 12,MIC =8 μg/mL) Pseudomonas aeruginosa were selected,and the bactericidal kinetics of 4 × MIC concentration of lycosin-Ⅰ against them were determined.After the representative isolates were cultured with suitable medium for 24 hours,the absorbency of 600 nm was detected and the growth curve was drawn.Five mmol/L of Ca2+ or Mg2+ were added into the medium to investigate the salt tolerance of lycosin-Ⅰ.Results Lycosin-Ⅰ in vitro showed good antibacterial activity against both multidrug resistant and non-multidrug resistant Pseudomonas aeruginosa.The MICs (median [P25,P75]) of lycosin-Ⅰ against multidrug-resistant and non-multidrug-resistant Pseudomonas aeruginosa were 12 (8,32) μg/mL,and there was no statistical difference between them (U =42,P ＞0.05).About 50％ of multidrug resistant and non-multidrug resistant Pseudomonas aeruginosa could be killed by 4 × MIC of lycosin-Ⅰ during 60 minutes.When the concentration of lycosin-Ⅰ below MIC,such as 0,2 or 4 μg/mL,was co-cultured with multidrug resistant and non-multidrug resistant Pseudomonas aeruginosa for 24 hours,the growth of bacteria was fast during 6 and 16 hours,and then tended to be slow after 18 hours.When the concentration of lycosin-Ⅰ increased to 8 μg/mL,the bacteria were hard to grow.The in vitro antibacterial activity of lycosin-Ⅰ could be reduced by the addition of 5 mmol/L of Ca2+ or Mg2+,and the MICs of lycosin-Ⅰ against multidrug resistant and non-multidrug resistant Pseudomonas aeruginosa increased from 8 μg/mL to 64 μg/mL for 5 mmol/L of Ca2+,and from 8 μg/mL to 32 μg/mL for 5 mmol/L of Mg2+,respectively.But high concentrations of lycosin-Ⅰ (＞ 64 μg/mL or ＞ 32 μg/mL) could still maintain its antibacterial activity against multidrug resistant and non-multidrug resistant Pseudomonas aeruginosa.Conclusion Lyosin-Ⅰ can effectively inhibit the growth of Pseudomonas aeruginosa in vitro,and has certain salt tolerance,which may be developed into a new type of antibacterial drug.

Comparative analysis of PET/CT and PET/MR image characteristics of head and neck squamous cell carcinoma / 中国医学影像技术

Objective To investigate PET/CT and PET/MR characteristics of head and neck squamous cell carcinoma (HNSCC).Methods Totally 40 patients with HNSCC underwent whole body 18F-FDG PET/CT and MR scans of head and neck before anti-tumor treatment.PET positive lesions of HNSCC,including primary lesions and lymph nodes were evaluated by 2 radiologists independently.Then the imaging quality,fusion quality,lesion conspicuity and lesion characteristics were assessed based on PET/CT,PET/MR T1WI and PET/MR T2WI.Results Ninety PET positive lesions in all 40patients were evaluated,including 40 primary lesions and 50 lymph nodes.Similar imaging quality and fusion quality of PET/CT,PET/MR T1WI and PET/MR T2WI were obtained without statistical difference (both P＞0.05).For the lesion conspicuity,PET/MR T1WI and PET/MR T2WI demonstrated significantly better than PET/CT in positive primary lesions and lymph nodes (all P＜0.05).For the characteristics of positive primary lesions,PET/MR T2WI provided more information than PET/CT in 29 lesions,equal to PET/CT in 4 lesions,and less than PET/CT in 7 lesions.Conclusion The application of PET/MR in HNSCC is feasible,being superior to PET/CT in indication of lesions in head and neck area.