Impact of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2 / 中华男科学杂志

<p><b>OBJECTIVE</b>To study the effect of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2.</p><p><b>METHODS</b>Recombinant plasmids of expressing short hairpin RNA targeting PC-1 mRNA were constructed using DNA recombinant technology and transfected into C4-2 cells via liposome. The positive cell clones were selected by G418. The expression of PC-1 gene was analyzed by RT-PCR and Western blotting technology. MTT and soft agar cloning formation were applied to observe the changes of the growth rate and independent anchor ability of C4-2 cells.</p><p><b>RESULTS</b>PC-1 RNA interference severely affected the expression of PC-1 gene and reduced the growth and colony formation ability of C4-2 cells.</p><p><b>CONCLUSION</b>RNA interference-mediated PC-1 gene knockdown can decrease the growth and cloning formation ability of C4-2 cells.</p>

Detection of bacterial 16S rRAN gene in EPS of men with chronic pelvic pain syndrome and its clinical significance / 中华男科学杂志

<p><b>OBJECTIVES</b>To investigate the cause of chronic pelvic pain syndrome (CPPS) and the correlation between presence of bacterial signal and the response to antibiotics by detecting bacterial 16S rRNA gene signal using polymerase chain reaction (PCR).</p><p><b>METHODS</b>EPS and first void urine (VB1) from 59 men with CPPS were analyzed by PCR for bacterial signal using universal primers specific for bacterial 16S rRNA gene. If the bacterial signal was found only in EPS or the EPS bacterial signal was at least 10 times stronger than the VB1 one, positive result could be decided for bacterial signal detection. All patients were treated with levofloxacin and azithromycin for 4 weeks. The treatment could be considered effective if more than 50% improvement, compared to the state before the treatment, was achieved in the symptom severity index(SSI), symptom frequency questionnaire(SFQ), national institutes of health chronic prostatis symptom index of pain(quasi-CPSI) or the patients' general subjective assessment.</p><p><b>RESULTS</b>Positive bacterial signal was detected in 46 of the 59 CPPS patients; The difference of improvement rates between positive bacterial signal group and negative bacterial signal group, which were 65%-74% and 0 respectively, was significant.</p><p><b>CONCLUSIONS</b>The basis of detecting 16S rRNA gene signal by PCR in clinical practice was built. The study shows that bacterial infection is related to CPPS. Bacterial signal detection results can help predict the effect of antimicrobial therapy.</p>