Comparison of genetic polymorphism of cytochrome CYP2C19 between men and women in Chinese population / 药学学报

<p><b>AIM</b>To assess the effect of gender on genetic polymorphism of cytochrome CYP2C19 in Chinese population.</p><p><b>METHODS</b>The genetic polymorphism of 140 healthy Chinese were analysed by PCR-RFLP (restriction fragment length polymorphism).</p><p><b>RESULTS</b>Of 52 genotyped male subjects, 23 (44.23%) were homozygous for wildtype (wt/wt), 6 (11.54%) were homozygous for CYP2C19 m1 (m1/m1), and 23 (44.23%) were heterozygous for CYP2C19 m1 or CYP2C19 m2 (wt/m1 or wt/m2); and among the 88 genotyped female subjects, 31 (35.23%) were homozygous for wildtype (wt/wt), 13 (14.82%) were homozygous for CYP2C19 m1 (m1/m1), and 44 (50.0%) were heterozygous for CYP2C19 m1 or CYP2C19 m2 (wt/m1 or wt/m2); no homozygous genotype for CYP2C19 m2 (m2/m2) was found in the study.</p><p><b>CONCLUSION</b>There is no statistical difference in ocurance of wt/wt and m1/m1 between in male and in female, so gender have no significant effect on genetic polymorphism of cytochrome CYP2C19.</p>

The plasma concentration of omeprazole and metabolites 5′- hydroxy omeprazole, omeprazole sulphone are determinated by HPLC / 中国药理学通报

AIM: To set up a method which can determining the blood concentration of omeprazole and its metabolite 5′-omeprazole, omerpazole sulphone in order to study its cinical pharmacokinetics. METHODS: The blood concentration of omerpazole was determinated by HPLC. RESULTS: Calibrated standard curve of omeprazole in blood is Y = -0.004 499 + 0.001 909X (r = 0.9990), the recoveries of three concentrations 50, 500, 2 000 mg·L-1 are 90.36, 109.62, 108.91%, respectively; and the precisions are 9.86, 7.86, 15.52%, respectively. Calibrated standard curve of its metabolite 5′-OH omeprazole in plasma is Y = - 0. 003 659 + 0. 001 328X (r = 0.9970), the recoveries of three concentrations 20, 200, 1 000 mg·L-1 are 79.42%, 90.49 %, 93.04%, respectively; and the precisions are 8.95%, 4.52%, 9.73%, respectively. Calibrated standard curve of its another metabolite omeprazole sulphone in plasma is Y = 0.009 248 + 0.001 765X (r = 0. 999 2), the recoveries of three concentrations 20, 200, 1 000 mg·L-1 are 94.44%, 105.59%, 104.26%, respectively; and the precisions are 8.72, 8.58, 9.60%, respectively. After 20 mg omeprazole were administered by a voluteer via oral, Cmax of 5′-OH omeprazole, omeprazole, and omerpazole sulphone were 14.622 7, 408.433 2, 454.363 7 mg·L-1. CONCLUSION: The method is good enough to study pharmacokinetics of omeprazole.