1.
China Pharmacy
; (12): 5147-5148, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-501326
ABSTRACT
OBJECTIVE:To establish a method to determine the content of chlorogenic acid in Yinhuang qinghuo capsule. METHODS:HPLC was performed on the column of Acclaim120? C18 with mobile phase of acetonitrile-0.4% phosphoric acid(12:88,V/V)at flow rate of 1 ml/min,the detection wavelength was 327 nm,column temperature was 25 ℃,and volume injection was 10 μl. RESULTS:The linear range of chlorogenic acid was 56-224 μg;RSDs of precision,stability and reproduciblity tests were lower than 3.0%,recovery was 96.91%-104.76%(RSD=1.46%,n=9). CONCLUSIONS:The method is simple and reproducible, and can be used for the quality control of Yinhuang qinghuo capsule.