ABSTRACT
Objective To explore the value of cardiac troponin Ⅰ (cTnI),brain natriuretic peptide (BNP),blood lactate and acute physiology and chronic health evaluation (APACHE) Ⅱ score which used to evaluate the severity of sepsis shock patients with myocardium injury.Methods Data of 61 patients with sepsis shock were collected,and cTnI,BNP,blood lactate were monitored and APACHE Ⅱ score were scored at admission,6,24 h after treatment.All of these indicators were compared between death group and survival group.Results In 61 patients,35 cases of them were lived (survival group),26 cases of them were died (death group).The cTnI,BNP,blood lactate and APACHE Ⅱ score at admission in death group was significantly higher than that in survival group (P＜ 0.05).In death group all of these indicators there was no significantly difference between admission and 6 h after treatment (P ＞0.05);24 h after treatment was significantly higher than that 6 h after treatment (P ＜ 0.05).In survival group 6,24 h after treatment was significantly lower than that at admission,24 h after treatment was significantly lower than that 6 h after treatment [BNP:(478.1 ± 95.3)ng/L vs.(1 025.2 ± 263.3) ng/L,cTnI:(0.9 ± 0.1) μ g/L vs.(2.5 ± 0.4) μ g/L,blood lactate:(2.4 ± 0.3) mmol/L vs.(5.4 ± 2.1) mmol/L,APACHE Ⅱ score:(11.4 ± 1.8) scores vs.(15.6 ± 2.9) scores] (P ＜ 0.05).Conclusion BNP,cTnI,blood lactate and APACHE Ⅱ score may be used to evaluate the severity of sepsis shock patients with myocardium injury and predict outcome of the patients.
ABSTRACT
Aim To explore the effect of microRNAs on the differentiation of 3T3-L1 adipocytes and the expression of adipo-related gene-fatty acid binding protein during the adipocyte differentiation.Methods adipo-related microRNAs during 3T3-L1 adipocyte differentiation were screened and identified by micorRNA microarray.Constructed high-expression plasmids of the adipo-related microRNAs,were transfected into the 3T3-L1 preadipocytes by lipofectamine.While the effect of adipo-related microRNAs on the course of 3T3-L1 adipocyte differentiation was observed,the protein and mRNA expression level of fatty acid binding protein(FABP4)were analyzed by Western blot and RT-PCR during 3T3-L1 adipocyte differentiation.Results The expression profiles of microRNAs have significant changed during 3T3-L1 adipocyte differentiation,in which 35 microRNAs among them down-relation,the most lowly expression is miR-24;17 microRNAs among them up-relation,the most highly expression is miR-21.MiR-24 significantly inhibited adipocyte differentiation and maturity,while miR-21 have no significant effect.MiR-24 significantly inhibited the expression of FABP4,but had no effect on the level of its mRNA;miR-21 had no effect on the expression of protein and mRNA of FABP4.Conclusion There exist adipogenic-related microRNAs during 3T3-L1 adipocyte differentiation; miR-24 play an important role in the regulation of 3T3-L1 preadipocyte differentiation into adipocyte and the(FABP4)protein expression.