ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of B7-H4 in prostate cancer tissue and the relationship between the expression and the clinicopathological features.</p><p><b>METHODS</b>Immunohistochemical staining was used to detect the expression of B7-H4 in prostate cancer tissue. And the relationship between the expressions and pathology was evaluated.</p><p><b>RESULTS</b>The B7-H4 was diffusely expressed in cytoplasm and/or membrane of the prostate cancer tissue; the expression was much higher than that in normal prostate tissue (P<0.05). The expression of B7-H4 in the prostate cancer tissue was higher in patients with higher tumor grade.</p><p><b>CONCLUSION</b>B7-H4 may be used as an new indicator for the diagnosis and prognosis of prostate cancer and a novel target for immunotherapy.</p>
Subject(s)
Aged , Aged, 80 and over , Humans , Male , Middle Aged , B7-1 Antigen , Metabolism , Biomarkers, Tumor , Metabolism , Prostate , Metabolism , Pathology , Prostatic Neoplasms , Metabolism , Pathology , V-Set Domain-Containing T-Cell Activation Inhibitor 1ABSTRACT
<p><b>OBJECTIVE</b>To construct a eukaryotic expression vector encoding the gene of extracellular region of mouse B7-H4, to express it in yeast cell line and to determine its biological activity.</p><p><b>METHODS</b>The extracellular region of the mouse B7-H4 gene was amplified with Xho I and EcoR I by PCR from a mouse B7-H4 chimeric plasmid. Digested with Xho I and EcoR I, the mB7-H4 gene was inserted into the yeast expression plasmid Ppic9. The DNA sequence was confirmed by double digestion and the Ppic9-mB7-H4 plasmid was transfected into the yeast cells. The expression of mB7-H4 was confirmed by PCR, Western Blot and ELISA analysis, and its biological function was determined.</p><p><b>RESULT</b>Ppic9-mB7-H4 transfectants expressed mB7-H4 in yeast cells, and mB7-H4 effectively inhibited the proliferation of T lymphocytes with a fractional inhibition rate of 28.3 % and inhibited IL-2, IL-4, IL-10 and IFN-gamma production with fractional inhibition rates of 68.8%, 78.8%, 67.6% and 77.7%, respectively.</p><p><b>CONCLUSION</b>The eukaryotic expression plasmid mouse B7-H4 has been successfully constructed and the expressed products of B7-H4 possess biological activity.</p>