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Objective:To preliminarily develop a fertility motivation scale for infertile women (FMS-IW) and test its reliability and validity.Methods:The FMS-IW was developed according to the theory of self-determination and Maslow's hierarchy of needs, and the original item pool was established through clinical psychological counseling practice experience, literature review, consulting clinical psychotherapists, interviewing with infertile women and open-ended questionnaires. The original scale was constructed on item analysis and exploratory factor analysis in 257 infertile women. The formal version of FMS-IW was further adapted by Delphi method and tested in another 392 women to conduct confirmatory factor analysis and reliability test. Furthermore, 56 participants of 392 women were randomly retested with FMS-IW after two weeks.SPSS 25.0 and Amos 24.0 software were used for Spearman analysis, exploratory factor analysis and confirmatory factor analysis.Results:Exploratory factor analysis showed that the FMS-IW was composed of 16 items consisting of two factors: autonomous fertility motivation and controlled fertility motivation. The cumulative variance contribution rate was 64.18%. Confirmatory factor analysis showed that the model fitted well ( χ2/ df=3.292, RMSEA=0.077, SRMR=0.055, GFI=0.902, AGFI=0.871, IFI=0.938, CFI=0.938, TLI=0.928). The Cronbach's α coefficient of the FMS-IW was 0.908. The Cronbach's α coefficient for autonomous and controlled fertility motivation was 0.911 and 0.928, respectively. The parity split-half coefficient of the formal version of FMS-IW was 0.870. The test-retest reliability of the formal version of FMS-IW was 0.823. Conclusion:The FMS-IW has good reliability and validity, and can be used as an effective tool to measure fertility motivation in infertile women.
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OBJECTIVE: To observe the efficacy and safety of tocilizumab against refractory systemic onset juvenile idiopathic arthritis(SoJIA). METHODS: A prospective follow-up study of 16 patients with refractory active SoJIA patients with or with tocilizumab after treatment of clinical disease activity indicators and safety. The ANOVA was used for statistical analysis. RESULTS: After 2 weeks,12 weeks and 52 weeks of treatment,the levels of white blood cells,erythrocyte sedimentation rate and hypersensitive C-reactive protein were significantly decreased,and there were statistically significant differences(F=26.25、145.70、517.96,P<0.05). JADAS27 was significantly decreased after 2 weeks,12 weeks and 52 weeks of treatment with tocilizumab(the score being 23.09±3.46,8.19±2.63,4.25±2.86 and 2.63±1.54),the difference being statistically significant(P<0.05). Some children had adverse reactions,2 cases of skin abnormalities,1 case of white pityriasis,1 case of skin infection;3 cases of liver enzyme abnormalities;2 cases of leukopenia. CONCLUSION: Tocilizumab can improve the condition of children with SoJIA in rapid and obvious way,and medium and longterm security and tolerance was good. The duration of tocilizumab therapy should be carefully assessed.
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The study is aimed to explore the metabolism rule of Lonicera japonica by investigating the primary and secondary metabolism process in different growth periods. HPLC and other methods were used to measure metabolism indexes of leaves collected in last ten days of every month. The results suggested that the maximum (78.59%) and minimum (60.83%) of water content were found in March and December. The content of total sugar reached a high level from December to February and the maximum (275.8 mg•g⁻¹) appeared in October, while it reduced significantly at other time. The change of chlorogenic acid, neochlorogenic acid, galuteolin, caffeic acid were basically consistent and the highest content of them synchronously appeared (42.79, 2.01, 7.13, 0.16 mg•g⁻¹) in March. The content of primary and secondary metabolite in L. japonica leaves reached a high level from March to May, and the main related elements with effective components were K, Mg, P, aspartate, threonine, proline, valine, cysteine, isoleucine and phenylalanine.
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To study the effects of different variable temperature drying modes on active components of roots of Salvia miltiorrhiza f. alba, and provide basis for its industrialized drying process. In order to ensure the content of active components, variable temperature drying modes were designed: low temperature at 30 ℃ and high temperature at 60 ℃, low temperature at 30 ℃ and high temperature at 70 ℃, low temperature at 30 ℃ and high temperature at 80 ℃, low temperature at 40 ℃ and high temperature at 60 ℃, low temperature at 40 ℃ and high temperature at 70 ℃, low temperature at 40 ℃ and high temperature at 80 ℃ and air dry oven was used for variable temperature drying process. Then HPLC method was used to determine the changes of active components in roots of S. miltiorrhiza f. alba under different temperature modes; and SPSS 17.0 was used to analyze the data. The results showed that the samples, which were first dried at 40 ℃ for six hours and then dried at 80 ℃ for three hours, had the highest contents in dihydrotanshinone, cryptotanshinone, tanshinone Ⅰ and tanshinone ⅡA as compared with other kinds of drying methods, and the contents were 0.35, 2.76, 0.78, 4.47 mg•g⁻¹, respectively. Additionally, as compared with samples dried in the shade, the contents of dihydrotanshinone, cryptotanshinone and tanshinone Ⅰ were increased 2.9% (P>0.05), 45.3% (P<0.05) and 34.5% (P<0.05), respectively; however, the content of tanshinone ⅡA was decreased by 44.1% (P<0.05). The water-soluble active components (rosmarinic acid and salvianolic acid B) of roots of S. miltiorrhiza f. alba, had the highest contents when the samples were first dried at 30 ℃ for six hours and then 70 ℃ for three hours, and the contents were 3.83,55.44 mg•g⁻¹, increased by 62.3% (P<0.05) and 109.1% (P<0.05) respectively as compared with the samples dried in the shade. Variable temperature drying can significantly affect the contents of active components in roots of S. miltiorrhiza f. alba. As compared with the traditional process of shade-drying process, low temperature drying can significantly increase the content of water-soluble active components and also with significant promotion effect on the liposoluble components such as tanshinone ⅡA, cryptotanshinone and tanshinone Ⅰ. The variable temperature drying mode, can effectively shorten the process of drying and provide theoretical basis for industrial processing of roots of S. miltiorrhiza f. alba.
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<p><b>OBJECTIVE</b>To analyze the expression of MicroRNA-15a (miR-15a) in children with primary immune thrombocytopenia (ITP) and its significance.</p><p><b>METHODS</b>The peripheral blood monomuclear cells (PBMNC) were isolated and cultured from ITP patients and healthy volunteers. The expression level of miR-15a was measured by real-time PCR. After miR-15a mimic was transfected into PBMNC, the levels of INF-γ, IL-2, IL-4 and IL-10 were measured by ELISA.</p><p><b>RESULTS</b>The expression of miR-15a was significantly decreased in PBMNC. The production of IFN-γ and IL-2 was dramatically increased, and the level of IL-4, IL-10 was decreased in PBMNC. Moreover, the expression of miR-15a was negatively correlated with IFN-γ and IL-2, and positively with IL-4 and IL-10. Furthermore, the results showed that the overexpression of miR-15a could decrease the production of IFN-γ and IL-2, and increase the production of IL-4 and IL-10.</p><p><b>CONCLUSION</b>miR-15a is significantly down-regulated in PBMNC of children with primary ITP and involved in the regulation of Th1/Th2 imbalance. It is suggested that miR-15a may be a potential therapeutic target for ITP.</p>
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Objective To investigate the clinical effect of intrathecal injection of ceftazidime and ceftriaxone in the treatment of intracranial infection after intraventricular drainage. Methods 60 patients with intracranial infection after intraventricular drainage in Wuzhong people's hospital from October 2013 to March 2017 were selected and were divided into reference group and study group by random sampling method, with 30 cases in each group. The reference group was treated with ceftriaxone treatment, the study group was treated with intrathecal ceftazidime and ceftriaxone. The therapeutic effect and adverse reaction rate of the two groups were evaluated. Results The total effective rate of the patients in the study group (96.67%) was significantly higher than that in the reference group (73.33 %), and the adverse reaction rate (3.33%) was significantly lower than that of the reference group (26.66%), and the difference was statistically significant (P<0.05). Conclusion Intrathecal injection of ceftazidime and ceftriaxone in patients with intracranial infection after intraventricular drainage, the treatment effect is obvious, and the incidence of adverse reactions is low, which is worthy of clinical study.
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Objective To investigate the clinical effect of intrathecal injection of ceftazidime and ceftriaxone in the treatment of intracranial infection after intraventricular drainage. Methods 60 patients with intracranial infection after intraventricular drainage in Wuzhong people's hospital from October 2013 to March 2017 were selected and were divided into reference group and study group by random sampling method, with 30 cases in each group. The reference group was treated with ceftriaxone treatment, the study group was treated with intrathecal ceftazidime and ceftriaxone. The therapeutic effect and adverse reaction rate of the two groups were evaluated. Results The total effective rate of the patients in the study group (96.67%) was significantly higher than that in the reference group (73.33 %), and the adverse reaction rate (3.33%) was significantly lower than that of the reference group (26.66%), and the difference was statistically significant (P<0.05). Conclusion Intrathecal injection of ceftazidime and ceftriaxone in patients with intracranial infection after intraventricular drainage, the treatment effect is obvious, and the incidence of adverse reactions is low, which is worthy of clinical study.
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Transcription factor E3 (TFE3) located in the X chromosome 11.2 plays an important role in cell growth, differentiation and transcription regulation. In the condition of pathology, the TFE3 gene translocates to form fusion genes, which makes the expression of TFE3 protein abnormally high, and affects the transcriptional regulation of cells and promotes the tumor formation. This article reviews the clinicopathological features of several TFE3 fusion genes related tumors,in order to improve the recognition of this disease.
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Objective To explore the influences of continued nursing care on the recovery of intra-articular fractures of joint function of limbs and the quality of life after operation. Methods A total of 493 intra-articular fracture of limbs postoperative patients,who were discharged from our department from January 2015 to December 2016,were randomly divided into observation group and reference group.After operation,the reference group used self-management mode to practice joint functional exercise,while the observation group uses continued nursing mode to guide physical therapy to recover joint functions.After they discharged 12 weeks,we compared these two groups of patients with satisfaction of nursing care,joint function recovery and quality of life. Results After 12 weeks of operation, in observation group, the patients' joint function recovery rate was close to 79.27%(195/246), which was significantly higher than the rates 55.47%(137/247)in reference group,the difference was statistically significant(χ2=32.947,P<0.01). Respectively, the physiological function score,vigor score, mental health score, physiology role score, body pain score, health condition score, social function score and health score of the observation group in the quality of life assessment were (72.66 ± 4.41), (89.27 ± 4.10), (93.01 ± 3.05), (88.03 ± 3.19), (91.68±3.99),(76.78±4.86),(79.11±4.68),(85.57±7.07).The scores of control groups were(65.71±3.41),(64.02±4.51),(61.43±4.29),(66.49±4.52),(64.24±4.30),(62.02±6.24),(62.94±4.50),(65.02±7.72).The comparative difference of the two groups has statistical significance (t=12.48- 60.15, P<0.05). Meanwhile,in the satisfaction rate of nursing care,the great satisfaction rate of observation group(70.73%, 174/246) was much higher than reference group (37.65%, 93/247), the difference was statistically significant (χ2=59.789, P<0.01). Conclusion Offered outside continued nursing care to the intra-articular fracture of limbs postoperative patients can effectively improve the joint function of patients,their qualities of life and can considerably increase satisfaction of nursing care. Thus,it should to be clinically promoted.
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This paper is aimed to study plant biomass and active compounds of Scutellaria baicalensis germchit in different five stages (from germination to transplant). The length of shoot and root, the diameter and the weight of root were determined. HPLC method was used to determine the content of active compounds (baicalin, scutellarin, wogonoside, baicalein, wogonin). According to the results, various biological indicators increased with the germination of seedling. However, the drying rate of the root declined to 27.96% from 32.90%. The contents of scutellarin and baicalein increased firstly, and then decreased. The maximums of them were 3.22,3.89 mg•g⁻¹ while the data of shoot/root was 0.35. The maximums of the contents of baicalin and wogonoside were 107.39,16.11 mg•g⁻¹ while the data of shoot/root was 0.23 and 0.06. The contents of wogonin gradually increased to the maximum of 0.88 mg•g⁻¹ while the data of shoot/root was 0.50. In conclusion, the contents of baicalin, scutellarin, wogonoside, baicalein and wogonin reached or approached the maximum at germination stage while the data of shoot/root was 0.35. The rate of shoot and root can be used as a judging index of active compounds for S. baicalensis germchit.
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Totally 80 rats were randomly divided into the control group, the model group, low, middle and high dose (25, 50, 100 mg x kg(-1)) scutellarin( SC) groups and the colchicine ( Col) group. Apart from the blank group, all of the remaining groups were intraperitoneally injected with 0.5 mL pig serum twice every week for consecutively 13 weeks and orally administered with the corresponding drugs since the 9th week. The blank group and the model group were orally given equal volume of normal saline once every for consecutively four weeks. After the experiment, efforts were made to detect the contents of alanine aminotransferase (ALT), aspertate aminotransferase (AST), albumin (ALB), total protein (TP), total bilirubin (TBIL), hyaluronic acid (HA), laminin (LN) and collagen type IV (CIV), collect liver tissues of fixed positions, observe the pathological changes through hematoxylin-eosin (HE) staining, conduct the pathological grading for liver fibrosis, determine the expressions of hepatic collagen type I and III (C I, C III) and calculate their color rendering index. Compared with the model group, low, middle and high dose (25, 50, 100 mg x kg(-1)) SC groups could decrease the contents of ALT, AST, TBIL, HA, LN, CIV, increase the contents of ALB, TP in serum and reduce the contents of C I, C III in liver tissues. In conclusion, scutellarin has a certain therapeutic effect on immune liver fibrosis in rats induced by pig serum.
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Animals , Humans , Male , Rats , Alanine Transaminase , Genetics , Metabolism , Apigenin , Bilirubin , Genetics , Metabolism , Collagen Type IV , Genetics , Metabolism , Drugs, Chinese Herbal , Glucuronates , Liver , Metabolism , Liver Cirrhosis , Drug Therapy , Genetics , Metabolism , Rats, Sprague-DawleyABSTRACT
<p><b>OBJECTIVE</b>To study the effect of aqueous extract of several kinds of herbs on human platelet aggregation and expression of P-selectin in vitro.</p><p><b>METHODS</b>Blood was collected from volunteers. Effects of the prepared water extracts of herbs on platelet aggregation were monitored on a Packs-4 aggregometer. The fluorescence intensity of water extracts of Caulis Spatholobi, Flos Carthami and Rhizoma Curcumae on the expression of P-selectin in human platelets of healthy persons was measured with flow cytometry.</p><p><b>RESULTS</b>Out of several herbs investigated, Flos Carthami and Rhizoma Curcumae potently inhibited platelet aggregation after incubation with platelet-rich plasma (PRP) for 15 min. Caulis Spatholobi Flos Carthami and Rhizoma Curcumae inhibited adenosine-5'-diphosphate (ADP) or platelet activating factor (PAF)-induced platelet aggregation in PRP in a dose-dependent manner. In contrast to Flos Carthami and Rhizoma Curcumae, Caulis Spatholobi could not inhibit thrombin-induced platelet aggregation. Despite its inability to inhibit thrombin-induced platelet aggregation in PRP, Caulis Spatholobi had a greater anti-aggregating activity in PRP induced by ADP or PAF. Caulis Spatholobi and Flos Carthami showed significant inhibitory effects on the expression of P-selectin.</p><p><b>CONCLUSIONS</b>Caulis Spatholobi, Flos Carthami and Rhizoma Curcumae have potent anti-platelet properties, and their inhibitory actions are mediated via different mechanisms. Caulis Spatholobi inhibited ADP-induced platelet aggregation but not by thrombin, indicating that its mechanism of action might be independent of the thromboxane pathway. The effect of Caulis Spatholobi and Flos Carthami were associated with suppressing the expression of P-selectin.</p>
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Adult , Humans , Young Adult , Blood Platelets , Metabolism , Curcuma , Chemistry , Fabaceae , Chemistry , P-Selectin , Metabolism , Plant Extracts , Chemistry , Pharmacology , Plants, Medicinal , Chemistry , Platelet Aggregation , Platelet Function Tests , Water , ChemistryABSTRACT
The change of yield and contents. of active compositions were studied while the fibrous roots were decayed naturally. HPLC method was used to detect the contents of active composition. The results show that fibrousroots could decrease the production of plant by 38.60% (20 g) and 30.99% (40 g), respectively. Treatment 1 could increase the contents of dihydrotanshinone and cryptotanshinone of Salvia miltiorrhiza f. alba by 26.08% and 22.64%, respectively. Compared with the comparison, treatment 2 decreased the contents of ihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone II(A) of S. miltiorrhiza f. alba by 60.87%, 79.24%, 84.61% and 88.99%, respectively. Meanwhile, the total contents of the liposoluble constituents reduced by 86.27%. The different concentration of fibrousroots could increase the content of salvianolic acid B by 4.98% (20 g) and 23.64% (40 g), respectively. Meanwhile, the content of rosemary acid was increased by 4.98% (20 g) and 23.64% (40 g), respectively. The content of water-soluble constituents positively correlated to the mount of added fibrousroots, and the change was significantly. The result indicted that the decay of fibrousroots has a significant impact on the growth and the content of the active composition of S. miltiorrhiza f. alba under the condition of continuous cropping. Fibrousroots could decrease the content of biomass and liposoluble constituents significantly, which maybe one of the main factors to S. miltiorrhiza f. alba continuous cropping obstacle formation.
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Benzofurans , Biomass , Chromatography, High Pressure Liquid , Abietanes , Plant Roots , Metabolism , Salvia miltiorrhiza , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To compare the clinical outcomes and cost-effectiveness of luteal phase down-regulation with gonadotrophin-releasing hormone (GnRH) agonist protocol and GnRH antagonist protocol in patients undergoing repeated in vitro fertilization and intracytoplasmic sperm injection (IVF-ICSI) cycles.</p><p><b>METHODS</b>A retrospective analysis of clinical outcomes and costs was conducted among 198 patients undergoing repeated IVF-ICSI cycles, including 109 receiving luteal phase down-regulation with GnRH agonist protocol (group A) and 89 receiving GnRH antagonist protocol (group B). The numbers of oocytes retrieved and good embryos, clinical pregnancy rate, abortion rate, the live birth rate, mean total cost, and the cost-effective ratio were compared between the two groups.</p><p><b>RESULTS</b>In patients undergoing repeated IVF-ICSI cycles, the two protocols produced no significant differences in the number of good embryos, clinical pregnancy rate, abortion rate, or twin pregnancy rate. Compared with group B, group A had better clinical outcomes though this difference was not statistically significant. The number of retrieved oocytes was significantly greater and live birth rate significantly higher in group A than in group B (9.13=4.98 vs 7.11=4.74, and 20.2% vs 9.0%, respectively). Compared with group B, group A had higher mean total cost per cycle but lower costs for each oocyte retrieved (2729.11 vs 3038.60 RMB yuan), each good embryo (8867.19 vs 9644.85 RMB yuan), each clinical pregnancy (77598.06 vs 96139.85 RMB yuan).</p><p><b>CONCLUSION</b>For patients undergoing repeated IVF/ICSI cycle, luteal phase down-regulation with GnRH agonist protocol produces good clinical outcomes with also good cost-effectiveness in spite an unsatisfactory ovarian reserve.</p>
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Adult , Female , Humans , Pregnancy , Costs and Cost Analysis , Ovulation Induction , Economics , Methods , Pregnancy Rate , Retrospective StudiesABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm.</p><p><b>METHODS</b>Different proportions of cryoprotectant to seminal plasma (1:1 and 1:3) were used for freezing sperm, and the forward movement and total motility rates of the frozen-thawed sperm were compared.</p><p><b>RESULTS</b>The forward movement and total motility rates were (58.60 +/- 5.57)% and (66.17 +/- 5.24)% before cryopreservation. The 1:1 proportion achieved post-thaw forward movement and total motility rates of (40.53 +/- 8.97)% and (51.23 +/- 9. 30)%, while the 1:3 (44.7 +/- 8.67)% and (51.50 +/- 7.40)%, respectively. Significantly decreased sperm motility was observed after cryopreservation (P < 0.05). Statistically significant differences were found in the forward movement but not in the total motility of the frozen-thawed sperm between the two proportions.</p><p><b>CONCLUSION</b>Cryopreservation causes obvious damage to human sperm. Higher proportion of cryoprotectant to seminal plasma (1:3) can improve the forward movement of post-thaw sperm as compared with the lower one (1:1).</p>
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Adult , Humans , Male , Cryoprotective Agents , Pharmacology , Freezing , Semen , Sperm MotilityABSTRACT
To assess the quality of randomized controlled trials (RCTs) published in Chinese Acupuncture and Moxibustion. Manually retrieve RCT papers published in Chinese Acupuncture and Moxibustion from 2000 to 2006 and use "the extraction form of acupuncture and moxibustion randomized clinical trial report data" to evaluate the quality of RCTs. Six hundred and eighty-six RCTs were enrolled. The methodological quality was lower than the international standard and only one RCT paper described the trial technological process. And there were a less reports about influencing RCT quality, such as acupuncturist's qualification and education background (3.06%), adverse reaction (4.23%), blind method (5.98%) and follow-up (14.43%). RCTs on acupuncture and moxibustion published in Chinese Acupuncture and Moxibustion need to be improved in trial design, conduction and report.
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Humans , Acupuncture Therapy , Moxibustion , Periodicals as Topic , Reference Standards , Randomized Controlled Trials as Topic , Reference Standards , Time FactorsABSTRACT
Objective To determine a suitable standard of hirsutism for Chinese polycystic ovary syndrome(PCOS)patients living in Shandong region.Methods A total of 623 unbiased women from the general population in Jinan city,131 PCOS patients and 84 controls from outpatients in Shandong region were studied with questionnaires,physical and pelvic ultrasound examination,body hair on 11 sites were evaluated,and 9(lip,chin,arm,thigh,chest,upperbelly,lowerbelly,upperback,lowback)of them which were called hormone Ferriman-Gallwey(F-G)score and 2(forearm,leg)sites of indifferent hormone score were calculated according to the score system described by Ferriman and Gallwey.Results(1)Both body hair F-G score and indifferent hormone score distribution mode in the≤40 years old population were un-normal and both the 95th percentages of score were 2.(2)The hirsutism was significantly higher in PCOS patients[48.1%(63/131)]than in controls[4.8%(4/84)]by F-G score≥2(X~2=47.68,P
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Objective To explore the clinical characteristics,diagnosis,treatment and misdiagnosis matter of children with idiopathic pulmonary hemosiderosis(IPH).Methods The data of clinical characteristics,laboratory examination,treatment and follow-up of 21 children admitted from Jun.1993 to May 2007 were retrospectively analyzed,included 9 males,12 females,aged 1-14 years old,course of di-sease were 1-6 months.Twenty-one patients were diagnosed as IPH by chest X-ray radiography,CT scan,bone marrow biopsy,hemosiderin-laden macrophages in either sputum or gastric juice or bronchoalveolar lavage fluid.Then,therapy and prognosis of IPH were analyzed.Results All patients had varied degrees of anemia,11(52.38%) children had cough,9 (42.86%) children had fever,6(28.50%) cases had shortage of orexia,4( 19.05%) children had hemoptysis.Chest X-ray radiography and CT scan demonstrated diffuse patchy,nodular,reticulate pattern.Eighteen children received bone marrow biopsy and presented hyperplastic erythropoiesis,4(22.2%) cases were accompanied iron deficiency anemia.Nineteen (90.80%) cases shown the presence of hemosiderin-laden macrophages in either sputum or gastric juice or bronchoalveolar lavage fluid.Twenty-one misdiagnosed patients consisted of bronchopneumonia combined anemia(8 cases),lung tuberculosis combined anemia(5 cases),nulli-iron anemia(4 cases),hemolytic anemia(3 cases),myelodysplastic syndrome(1 case)and received corticosteroid therapy.Four cases of all patients were associatated with large-dose human-?-globulin and 3 cases were associatated with vincristine therapy.The therapeutic effect was significant.Eighteen patients were followed-up,3 patients were of which cured and had stopped treatment for over 2 years,11 patients presented clinically persistent remission,4 patients were recurred and aggravated.Conclusions Early diagnosis and long-term therapy of corticosteroid are very important for controlling acute onset,lessening the frequency of IPH recurrence and improving prognosis of the disease.