ABSTRACT
OBJECTIVE@#To explore the mechanism of tauroursodeoxycholic acid (TUDCA) in suppressing apoptosis in pulmonary tissues of intermittent hypoxia (IH) mice model. @*METHODS@#A total of 32 C57 mice were randomly divided into a control group, a TUDCA group, an IH group and an IH+TUDCA group (8 mice per group). The mice were put in specially designed chambers and exposed to IH treatment for 4 weeks. In the chambers, oxygen levels repeatedly decreased from 21% to 10% and recovered from 10% to 21%, lasting for 8 hours in every day. After 4 weeks of IH exposure, the expression levels of caspase-12 and cleaved caspase-3 in pulmonary tissues were detected by Western blot. Meanwhile, the expression levels of glucose regulated protein-78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP) were quantified by Western blot, immunochemistry and real-time PCR. @*RESULTS@#Compared with the control group, the expression levels of caspase-12, cleaved caspase-3, GRP78 and CHOP were increased in the IH group (all P<0.01). TUDCA treatment could reduce these proteins expression (all P<0.05). @*CONCLUSION@#Endoplasmic reticulum stress-mediated apoptosis can be activated in pulmonary tissues after chronic IH exposure, and TUDCA can reduce the cellular apoptosis via suppressing endoplasmic reticulum stress.
Subject(s)
Animals , Mice , Apoptosis , Caspase 12 , Metabolism , Caspase 3 , Metabolism , Disease Models, Animal , Endoplasmic Reticulum Stress , Heat-Shock Proteins , Metabolism , Hypoxia , Lung , Mice, Inbred C57BL , Real-Time Polymerase Chain Reaction , Taurochenodeoxycholic Acid , Pharmacology , Transcription Factor CHOP , MetabolismABSTRACT
Objective To investigate the expression of CD34 and its clinical significances in different types of thymic tumor.Methods The expressions of CD34 in 61 thymic tumor tissues were detected by SP immunohistochemical method,then,the MVD was calculated.The patients included 7 cases of type A,9 cases of type AB,16 cases of type B1,10 cases of type B2,10 cases of type B3 and 9 cases of type C according to 2004 WHO Classification of thymic tumor.Results CD34 expression level was significantly increased from type A to type C.The MVDs were 3.78±2.12,5.72±2.79,7.51±3.34,10.89±5.10,12.31±4.08 and 16.51±6.24 in A,AB,B1,B2,B3 and C type of thymic tumor (F =2.048,P =0.047).The expression level in B2+B3+C type was significantly higher than A+AB+B1 type (t =6.034,P ＜ 0.05).Conclusions The expression of CD34 in different types of thymic tumor suggests that it may take some action in tumor-associated angiogenic function.Detection of CD34 may be useful to evaluate malignancy of thymic tumor.
ABSTRACT
BACKGROUND: Studies have demonstrated that trehalose possesses protective effects on cyropreserved sternum. But the mechanism of action remains poorly understood. OBJECTIVE: To investigate the effects of trehalose on bcl-2 and bax mRNA expression in cryopreserved sternum. METHODS: Four groups of freshly prepared solution were used: low-potassium dextran (LPD), LPD + dimethyl sulfoxide (DMSO), LPD + trehalose, LPD + DMSO + trehalose. Rat sternum was cut and then immediately cryopreserved in the tubes containing each group of solution. Fresh rat sternum tissue and 4 groups of samples cryopreserved for 120 days were taken and bcl-2 and bax mRNA expression in fresh and cryopreserved sternum was detected using reverse transcription-polymerase chain reaction.RESULTS AND CONCLUSION: bcl-2 mRNA expression in the LPD + trehalose group was significantly higher, but bax mRNA expression was significantly lower, than in the LPD, LPD + DMSO groups (both P 0.05). These findings indicate that trehalose may protect cell activity in cryopreserved sternum by enhancing bcl-2 mRNA expression and inhibiting bax mRNA expression, and trehalose together with DMSO shows better protective effects.
ABSTRACT
Objective To investigate the effect of isoflurane inhalation during gestation period on plasticity of hippo-campal synapses in offspring rats. Methods Ten healthy pregnant SD rats at 14 day gestation were randomly divided into 2 gorups ( n = 5 each): control group (group C) and isoflurane group (group I). The rats in group C were mechanically ventilated with O2 while in group I the rats inhaled 1.3% isoflurane in O2 for 2 h a day until labor. Four weeks after birth 4 offspring rats from each pregnant rats (2 male, 2 female) were tested for learning and memory abilities using Morris water maze. Then the offspring rats were sacrificed and hippocampi isolated. The synaptic structure of hippocampal CA1 area was examined by trans-electron microscopy. Results Morris water maze test showed that the escape latency was significantly shorter and the number of times of spanning flat roof greater in group C than in group I. The structure of hippocampus was intact in group C but incomplete in group I. Meanwhile the thickness of synaptic density was significantly decreased in group I. Conclusion Isoflurane anesthesia of pregnant rats may induce learning and memory disabilities in offspring rats by inhibiting the plasticity of synaptic structure in hippocampus.