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Objective To analyze the distribution and drug resistance of Streptococcus pneumoniae in 0-14 year old children of 0 to 4 year-old in theour hospital.Methods S.pneumoniae were identified by using ma-trix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).Drug suscepti-bility test was performed by using ATB STREP 5 for each isolate.Penicillin G minimum inhibitory concentra-tion (MIC) was performed by using E-test.Results A total of 327 strains of S.pneumoniae were isolated,a-mong which 287(87.8%)were from sputum,25(7.6%) from the ear secretions,10(2.9%) from the nasal sinuses puncture fluid,and 3(0.9%)from the blood.The most strains were isolated in spring(28.4%),and the least in winter (22%),and there was no significant difference in the separation rate among the four sea-sons (P<0.05).The sensitivity rate,intermediate rate and drug resistant rate of non meningitis S.pneumoni-ae strains to penicillin G were 97.9%,0.6%,1.5%,respectively.The resistance rates of the bacteria to eryth-romycin,clindamycin,compoundtrimethoprim sulfamethoxazole and tetracycline were relatively high,a small number of levofloxacin resistant strains were found,and vancomycin and quinopin /Dafoe-P resistant strains were not detected.The resistance rates of penicillin G,penicillin resistant Streptococcus pneumoniae (PRSP) and penicillin inter mediated Streptococcus pneumoniae (PISP)to amoxicillin and cefotaximeto were markedly higher than that of penicillin G sensitive Streptococcus pneumoniae (PSSP),and the difference was statistically significant (P<0.01).A total of 227 strains of multidrug resistance Streptococcus pneumoniae (69.4%)were isolated,which were resistant to erythromycin,tetracycline,trimethopri compoundm-sulfamethoxazol and clin-damycin(46.2%).Conclusion The drug resistance monitoring of Streptococcus pneumoniae in children can not be ignored.The drug resistance of the bacteria to penicillin G is low.Penicillin G is still a cheap and effec-tive drug for the treatment of Streptococcus pneumoniae infection.
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OBJECTIVE: To identify and analyze drug sensitivity of Corynebacterium glucuronolyticum iscolated from clinic, and to provide reference for clinical drug use. METHODS: Two strains isolated from the urine specimens of urolithiasis-induced urinary tract infection patients in our hospital were inoculated into Columbia blood plate and the MacConkey plate. The growth of strains was observed and counted. Protein mass spectrometry of strains was detected by MALDI-TOF-MS. DNA of strains was extracted, and PCR was used to amplify the 16S ribosome RNA (rRNA) sequence. Bi-directional sequencing of 1 500 bp target bands was conducted. Blast comparison between it and GenBank database was conducted to identify bacterial strain. Drug resistance of 2 strains was monitored by Etest assay. RESULTS: Two strains grew on the Columbia blood plate (with colony forming unit ＞105 CFU/mL) and did not grow on the MacConkey plate. Two strains were Gram-positive Corynebacterium and showed palisading or eight type arrangement. Two strains were C. glucuronolyticum by MALDI-TOF-MS identification, with reliability of 99. 9％. The characteristic peaks of m/z 2 431, 3 089, 3 364, 3 378, 4 200, 5 508, 6 302, 6 637, 6 730, 6 946, 12 603 appeared. Blast comparison showed that the sequence homology of 2 strains compared with C. glucuronolyticum strain known in GenBank were higher than 98 ％. Results of drug sensitivity test showed that strain 1 was resistant to ceftriaxone and ciprofloxacin, and sensitive to 14 other antibiotics as penicillin G; strain 2 was resistant to ceftriaxone, erythromycin, ciprofloxacin, tetracycline and clindamycin, moderately sensitive to cefotaxime, and sensitive to 10 other antibiotics. CONCLUSIONS: Two strains are C. glucuronolyticum, and drug resistance of them to commonly used antibiotics is different. The strains are rare pathogen of urinary tract and show multidrug resistance. Antibiotics should be selected according to the results of strain identification and drug sensitivity test.
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Objective To investigate the antimicrobial resistance of bacterial isolates in Liuzhou Workers' Hospital in Guangxi during 2016.Methods Kirby-Bauer method or automated systems was employed to study the antimicrobial susceptibility.The data were analyzed according to CLSI 2015 breakpoints.Results A total of 5 485 bacterial strains were isolated from 48 908 specimens in Liuzhou Workers' Hospital during 2016,of which gram negative organisms accounted for 70.3％ and gram positive cocci 29.7％.The prevalence of methicillin-resistant strains was 18.3％ (83/454) in S.aureus and 72.5％ (232/320) in coagulase negative Staphylococcus.No staphylococcal strains were found resistant to vancomycin or linezolid.Of the 207 strains of non-meningitis S.pneumoniae,181 (87.4％) were isolated from children and 26 (12.6％) from adults.One E.faecium strain was identified as resistant to vancomycin.The prevalence of ESBLs-producing strains was 49.0％ (619/1 263) in E.coli and 31.8％ (202/636) in Klebsiella spp.Enterobacteriaceae strains were still highly susceptible to carbapenem antibiotics.Overall,0.6％ (14/2 326) and 1.5％ (36/2 326) of the Enterobacteriaceae strains were resistant to imipenem and meropenem,respectively.Conclusions Antibiotic-resistant bacterial strains,especially carbapenem-resistant Enterobacteriaceae,are posing an increasing threat to clinical practice.Special attention should be paid to ongoing surveillance of local antimicrobial resistance to inform standardized anti-infective treatment.
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OBJECTIVE To understand the distribution and the drug resistance of the pathogenic bacteria so as to guide rational application of antibiotics for clinics.METHODS Bacteria culture and drug sensitivity with urine specimen were done among 1513 cases with urinary tract infection.RESULTS Urinary infection was mostly due to Gram-negative bacilli.Gram-positive cocci and fungi were the second.The top four pathogens were Escherichia coli(47.5%),Enterococcus faecium(8.7%),Candida albicans(7.0%),and Klebsiella pneumoniae(6.5%).The positive rates of urinary smear and culture were 51.9% and 29.3%,respectively with a coincidence rate of 77.4%.The drug resistance rates against imipenem,vancomycin and linezolid were zero,with 19.3% and 34.5% against extended-spectrum ?-lactamases-producing E.coli(ESBLs) and K.pneumoniae together,with 33.3% and 58.2% against meticillin-resistant coagulase negative Staphylococcus(MRCNS) and high-level gentamicin resistance(HLGR).CONCLUSIONS Urinary smear,bacteria culture and drug sensitivity tests can offer the basis for clinical rational use of antibiotics.
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0.05). CONCLUSIONS Minocycline,deoxycycline and josamycin can be chosed to cure Mycoplasma infection in this territory. Drug fast rate of mycoplasma is changing with the time. It is important for guiding clinic to monitor drug resistance of mycoplasma of Genitourinary tract.
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OBJECTIVE:To study the value of bronchoalveolar lavage fluid(BALF) bacteria culture and drug sensitivity test in the hospital-acquired pneumonia(HAP) therapy.METHODS:The bacteria types in BALF samples and drug sensitivity test in 516 HAP cases were analyzed retrospectively.RESULTS:The positive rate of BALF culture was 46.9% with the main pathogenic bacteria of Candida albicans,Acinetobacter baumannii,Pseudomonas aeruginosa,Klebsiella pneumoniae.Of total nosocomial pneumonia,single bacteria infection accounted for 58.9% and mixed infection 41.1%.Candida had no drug resistance against 5-fluorocytosine,amphotericin B.Extended spectrum ? lactamase was found in 75.9% of Escherichia coli and 35.7% of Klebsiella pneumoniae with severe drug resistance.CONCLUSIONS:BALF bacteria culture and drug sensitivity test can provide reference for rational use of drug in the clinic.
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OBJECTIVE:To observe the effect of Buflomedil Injection plus backwards traction and functional exercises on the hemorheology of patients with vertebral artery type of cervical spondylosis. METHODS:Sixty patients with vertebral artery type of cervical spondylosis were treated with 0.2 g Buflomedil Injection (diluted with 500 mL 0.9% Sodium Chloride Solution or 5% Glucose Solution,q.d by slow iv gtt for two courses of treatment with 7 days defined as 1 course) plus backwards traction and functional exercises. Patients' hemorheologic parameters including blood viscosity high shear rate,blood viscosity low shear rate,plasma viscosity,erythrocyte aggregation index,erythrocyte rigidity index,hematocrit,and fibrinogen etc were detected before and after treatment. RESULTS:Before treatment,all the hemorheologic parameters were significantly higher than the reference values of healthy adults(P
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OBJECTIVE To explore the relationship between Ureaplasma urealytium(Uu),Mycoplasma hominis(Mh),and Chlamydia trachomatis(Ct) with infertilitas feminis.The drug sensitivity can offer evidence for rational usage to improve the cure rate.METHODS Of 320 cases of infertility womenthe,primary infertility accounted for 132 cases and the secondary infertility for 188 cases.Other 120 cases of normal women were chosen as control.Secretion samples were used to detect Uu,Mh,Ct,and drug sensitivity.RESULTS The rate of Uu,Ct,Uu+Ct,Uu+Mh in infertility group was significant higher than control group(P0.05);minocycline doxycycline,josamycin and erythromycin cyclocarbonte were sensitive to Uu+Mh;drug resistance rate was higher in Uu+Mh infection, multidrug resistance was very serious.CONCLUSIONS Uu,Mh and Ct infections are the major factors to infertilitas feminis.The detection of Uu,Mh and Ct is good for diagneosis.
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OBJECTIVE:To study the mycoplasma infection and the drug susceptibility of mycoplasma in cervical secretions from female genital tract in our hospital.METHODS:Ureaplasma urealyticum(Uu)and mycoplasma hominis(Mh)in cervical secretions sampled from 3 416 female genital tract inflammation cases in our hospital were identified by cultivation,and the antibiotic resistance was also detected.RESULTS:The total positive rate of mycoplasma was 43.7%;Uu infection accounted for 40.0%,which was sensitive to Cycloate erythromycin,Minocycline,Deoxycycline,Josamycin;Mh infection accounted for 0.7%,which was sensitive to Deoxycycline,Minocycline and Josamycin;Uu+Mh infection accounted for 3.0%,which was sensitive to Deoxycycline,Josamycin and Minocycline.CONCLUSION:Rational use of drugs based on drug susceptibility test is of importance to prevent the production of persister of mycoplasma.
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This study was designed to determine the protective effect of scopolamine and catalase on the arterial endothelial cells during reoxygenation. The culture of endothelial cells from bovine aorta subjected to ischemia for 2 hours and reoxygenation for 30 minutes was used as a model for inves-tigaion. In the experimental group, scopolamine was added to the arterial endothelial cells culture medium, Hanks solution containing no sugar or oxygen; or in the control group no medicine was added to the ordinary Hanks solution. The alteration of glutathione ( GSH ) and malondialdehyde (MDA) of endothelial cells, and nitric oxide (NO) and lac-tate dehydrogenase (LDH) in the supernatant fluid were determined. The results demonstrated the following changes: (1) GSH in the control group significant reduction from the preischemia value 44. 5 + 2.45 to postischemia and reoxy genation value 35.08 + 5.72ug/mg protein, vs the post-ischemia and reoxygenatior value of 46. 67+3.20ug/ mg protein (scopolamine) and 43.38+2.98ug/mg protein (catalase). The differences between the GSH changes of control group and medicine treated group are statistically significant (P