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Objective:To investigate the effect of peppermint decoction spray on thirst distress and quality of life of patients with chronic heart failure.Methods:By convenient sampling method, a total of 122 chronic heart failure patients admitted to Guangzhou Red Cross Hospital from January to September 2020 were enrolled, who were assigned to experimental group and control group, 61 cases, respectively. Routine nursing and volume management were implemented in the two groups. The control group was treated with pure water spray, while the experimental group implemented peppermint decoction spray therapy, continuous treatment for 10 days. The clinical effect was assessed by Thirst Distress Scale for Patients with Heart Failure (TDS-HF) and Minnesota Living with Heart Failure Questionnaire (MLHFQ), respectively.Results:After 5 days and 10 days of treatment, the scores of TDS-HF were (12.80 ± 3.29) and (11.82 ± 2.63) in the experimental group, lower than that in the control group (15.29 ± 4.26) and (14.26 ± 3.74), the difference was statistically significant ( t=3.48, 4.04, both P<0.01); after 10 days of treatment, the physical dimension, emotional dimension and total MLHFQ scores were (56.12 ± 9.22), (51.21 ± 12.83) and (52.18 ± 6.09) in the experimental group, higher than that in the control group (50.82 ± 9.84), (49.10 ± 11.72) and (47.83 ± 5.44), the difference was statistically significant ( t=2.96, 3.53, 4.02, all P<0.05). Conclusions:Peppermint decoction spray therapy can effectively alleviate thirst distress and promote quality of life of patients with chronic heart failure.
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Objective:To investigate the mechanism of Wnt5a in lentoid body (LB) induction from human embryonic stem cells (hESCs).Methods:A "three-stage" protocol was used for LB differentiation from hESCs in vitro, and Wnt5a level was modified by adding exogenous 500 ng/ml Wnt5a on day 18 as Wnt5a treatment group.Cells of control group and Wnt5a treatment group were collected on day 35.Cells were photographed by using the Zeiss Axio Observer Z1 microscope.Transcriptome sequencing was applied by Illumina.Genes with P value≤0.05 and fold change≥1.5 were identified as differentially expressed genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to determine the biological functions of DEGs. Results:Compared with the control group, larger lentoid bodies were obtained in the Wnt5a treatment group.Transcriptome sequencing result showed that 478 genes were down-regulated and 201 genes were up-regulated in the Wnt5a treatment group compared with the control group, and Wnt5a up-regulated both lens cell differentiation and lens specific gene expression.Bioinformatics analysis result showed that most DEGs were involved in extracellular matrix remodeling, suggesting that Wnt5a regulated extracellular matrix remodeling during lens cell differentiation.The enrichment analysis result also showed that epithelial-to-mesenchymal transformation related processes were inhibited after Wnt5a treatment, suggesting that Wnt5a inhibited the abnormal differentiation of lens cells (especially lens epithelial cells) during lens cell differentiation.Wnt5a influenced the processes related to cytoskeleton remodeling.Conclusions:Wnt5a may act in lens cells through MAPK/ERK signaling pathways to affect ECM and cell cytoskeletal organization, which provides a new direction for studying lens development.
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Objective To observe the expression dynamics of lens-related transcription factors in human embryonic stem cell (hESC) differentiated into lentoid body(LB).Methods A "three-stage" protocol was used for LB directional differentiation from hESC in vitro.The hESC (D0) and three differentiation stages cells were collected to analyze the expression dynamics of lens development-associated transcription factors by high throughput RNA sequencing technology in hESC-induced LB.Western blot and cell immunofluorescence were used to observe the involved genes at protein level.Results During D0-D6,cells became more round and compact.And during D7-D18,cells morphology gradually changed to spindle.At the end of D35,three-dimensional and transparent structurelentoid body (LB) was obtained.RT-PCR results showed that the stem cell related genes reduced and the lens specific genes increased significantly,and the LB was characterized by the expression of crystallins.According to clustering analysis of high throughput sequencing,a distinct difference in transcription factors gene expression was observed between D0 and D32.Meanwhile,the difference between D6 and D18 was minimum.The expressions of preplacodal genes,including DLX3,DLX5,DLX6,HES1,HES4,OTX2 and EYA1 increased remarkably at the first induction stage and then decreased.Lens-specification gene SOX2 declined gradually and then increased.In addition,the expression of PAX6 increased during all three induction stages.Furthermore,lens-differentiation genes including MAB21L1,CMAF,PROXI and PITX3 had no significant change in the early induction stage,but increased significantly at the third induction stage.Conclusions The expression dynamics of lens development-associated transcription factors in the hESC induced LB corresponded to those in vivo,which indicate that this induction system can recapitulate early lens development well and lay the foundation of studying lens embryonic development andtranscription factor associated congenital lens diseases.
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Objective To study the effect of basic fibroblast growth factor (bFGF) on proliferating of rabbit lens epithelial cells(RLECs).Methods The second and three generations of RLECs were exposed to different concentrations of bFGF, and the proliferation characteristics of the cells were measured with methyl thiazolyl tetrazolium (MTT) assay. The ultrastructure of cell were observed with transmission electron microscope (TEM).The changs of cells cycles were observed with flow cytometer (FCM).Results After the treatment of bFGF, RLECs showed the marked proliferation , especially in 10μg*L-1 of bFGF . TEM result showed that the cells were more active with bFGF, FCM result showed that the S phase cell obviously increased.Conclusion bFGF is an important factor that can promote proliferation of RLECs.