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Cancer stem cells are currently under intensive investigation due to their capabilities for tumor initiation, self-renewal, and resistance to chemotherapy. CD133 is implicated in stemness and the malignancy of tumor cells. Here, we explored heat shock protein gp96 adjuvanted CD133 epitope vaccine against leukemia. We screened and identified three H2-Kd-restricted cytotoxic T lymphocyte (CTL) epitopes derived from CD133, CD133₄₁₉₋₄₂₈, CD133₇₀₂₋₇₁₀ and CD133₇₆₀₋₇₆₉. The immunogenicity and antitumor activity of the epitope vaccine using heat shock protein gp96 as adjuvant were further determined in CD133⁺ leukemia xenograft mice. Finally, we demonstrate that adoptive transfer of epitope-specific CTLs led to suppression of leukemia growth. Our data therefore provide the basis for designing a CD133 epitope vaccine to activate specific CTLs against CD133⁺ leukemia and other cancers.
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Objective To summarize the radiographic characteristic and endovascular interventional technique in patients with intracranial vertibrobasil dissectimng aneurysm.Methods Totally 30 patients of intracranial vertibrobasil dissectimng aneurysm were embolized by endovascular therapy,4 with stent implantation technique,26 with stent assisted coiling technique.Results Patency of parent artery was restored in 25 cases(83.3%),and except 1 case(3.3%) of postoperative intracerebral hemorrhage,no other complications occurred.All patients were followed up from 5 months to 34 months(average 11.2).All cases recovered well.No case died in the cohort.Conclusion Intracranial vertibrobasil dissectimng aneurysm has following angiographic features: string sign,pearl and string sign,and double lumen sign.Therapeutic strategies depend on clinical symptoms,the location,configuration of the dissection,and the relationship of vital branches around.The results suggested that embolization of intracranial vertibrobasil dissectimng aneurysm with coils is minimally invasive,safe,effective and reliable.
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Objective To investigate protein kinase A (PKA) signal regulatory mechanism of corticotropin-releasing hormone (CRH) mRNA expression, after CRH stimulated neuron of hypothalamic slices in rats in vitro. Methods After CRH stimulated corticotropin-releasing hormone type 1 receptor (CRH1R) of hypothalamic slices in rats in vitro, the changes of activity of PKA signal pathway and their relationship with CRH mRNA expression were observed by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting. Results CRH may cause the remarkable increase in phosphorylated PKA (P-PKA), phosphorylated cyclic adenosine monophosphate response element-binding protein (P-CREB) and CRH mRNA content in hypothalamic slices in rats. However, CP-l54526 or H89 could have significant inhibition effects on the synthesis of P-PKA, P-CREB and CRH. Conclusion PKA signal pathway in ultrashort positive feedback control of CRH secretion in hypothalamus in the stress due to severe traumas.
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Objective To investigate the signal regulatory mechanism of expression of corticotropin releasing hormone (CRH) stimulated neurons of hypothalamic slices in rats in vitro . Methods Model of hypothalamic slices of rats was established. After CRH stimulatation of corticotropin releasing hormone type 1 receptor (CRH1R) of hypothalamic slices in rats in vitro , the changes of activity of protein kinase A (PKA) signal passway were observed by immunocytochemical method and Western blotting. The relationship between the changes and CRH mRNA expression was also observed. Results CRH could cause the remarkable increase in phosphorylated PKA, phosphorylated CREB, and CRH content in hypothalamic slices in rats. However, CPl54526 or H89 could have significant inhibitory effect on the synthesis of P PKA, P CREB, and CRH. Conclusion PKA signal passway can regulate the ultrashort positive feedback of CRH secretion in the rat hypothalamus in stress due to severe trauma.
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Objective To evaluate the therapeutic efficacy of gamma knife for the treatment of idiopathic trigeminal neuralgia Methods An analysis of 87 cases with idiopathic trigeminal neuralgia treated by gamma knife in our department in the past 5 years was performed In all cases, 4 mm isocenter was targeted at the proximal nerve at the root entry zone located by MRI The target dose varied from 70-90 Gy Results After a follow up of 6-67 months, complete relief of pain occurred in 66 patients (75 9%), 50%-90% relief in 18(20 7%), relief less than 50% in 2(2 3%) but no relief in 1(1 1%) Repeated radiosurgery was performed in 2 patients (2 3%) with recurrent pain After the second radiosurgical procedure, complete relief of pain was found in the 2 patients Two patients(2 3%) experienced facial numbness after radiosurgical procedure Conclusion Gamma knife radiosurgery is a minimally invasive technique for the treatment of trigeminal neuralgia with few complications Repeated radiosurgical procedure may result in relief of pain, but the long term outcome of gamma knife surgery for trigeminal neuralgia needs to be further studied
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Objective To study and assess the therapeutic efficacy of Gamma knife on pituitary adenoma. Methods An analysis of 145 cases with pituitary adenoma treated by Gamma knife in our department in the past 4 years was performed. Adenoma diameter varied from 2.8 to 46.9 mm, averaging 15.6mm. In all cases, 4 mm and 8 mm isocenters were targeted at pituitary adenoma located by MRI and ? plan network. The target margin dose varied from 8 to 36 Gy, averaging 22.7 Gy (nonfunctioning adenomas, 18.5 Gy; functioning adenomas, 24.1 Gy). Results The follow up of 124 cases after radiosurgery varied from 6 to 56 months. Pituitary adenoma of 24 patients(19.4%) disappeared, 95 (76.6%) decreased, and 1 (76.6%) increased. Endocrinological normalization rate was 13 cases (13.3%), and a significant decrease of excessive hormone production was found in 71 patients (72.4%). Amelioration of clinical symptom was found in 114 patients (91.9%), deterioration in 4 patients (3.2%). Postradiosurgical complications were found in 2.4%. Neither optical lesion nor death was found in all cases. Conclusions Gamma knife radiosurgery is a minimally invasive technique to treat pituitary adenoma with satisfactory therapeutic efficacy. It is associated with a low risk of complication. Grasping adaptive sign strictly and planning precisely are needed to decrease complication.
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Objective:To study the diagnosis of magnetic resonance imaging(MRI)and the effect of microsurgical treatment for Arnold-Chiari malformation.Methods:Retrospective analysis of clinical data of 35 patients with Arnold-Chiari malformation.Results:Thirty-five cases were diagnosed by MRI.Posterior fossa decompression and plastic operation of cistern magna was performed in 35 cases.30 in all cases were followed up from 3 months to 7 years.Postoperative MRI revealed the size of the syrinx cavity decreased in all cases,disappeared in 5 cases.Conclusion:MRI is the best diagnostic method for Arnold-Chiari malformation.The effect of post fossa decompression and plastic operation of cistern magna for Arnold-Chiari malformation is satisfactory.