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Objective To investigate the effect of open surgical drainage approach for the treatment of walled-off pancreatic necrosis ( WOPN) in severe acute pancreatitis. Methods Clinical data of 154 WOPN patients admitted in the First Affiliated Hospital of Xinjiang Medical University from January 2005 to October 2016 were retrospectively analyzed. Traditional open debridement necrosectomy was performed in 83 patients from January 2005 to October 2012 ( debridement group) , and small abdominal incision with low-position open surgical drainage was performed in 71 patients from October 2012 to October 2016 ( drainage group ) . The clinical outcomes of two groups were analyzed and compared. Results 43 cases (51. 8％) in debridement group had postoperative intraperitoneal reinfection, while there were only 13 cases with postoperative intraperitoneal reinfection (18. 3％) in drainage group;18 cases (21. 7％) in debridement group had surgery-related digestive tract fistula, while there were only 4 cases with surgery-related digestive tract fistula (5. 6％) in drainage group; the differences were statistically significant (χ2 = 18. 55, P=0. 001; χ2 = 11. 35, P=0. 002). 15 patients (18. 1％) in debridement group and only 2 patients (2. 8％) in drainage group died. The mortality in drainage group were obviously lower than that in debridement group, and the difference was statistically significant (χ2 = 9. 07, P<0. 05 ). 62 cases ( 74. 7％) in debridement group and 55 cases (77. 5％) in drainage group were cured directly, respectively. No significant difference was found between two groups. However, 3 cases (3. 6％) in debridement group and 12 cases (16. 9％) in drainage group were cured by the way of small intestinal fistula in the late stage of intubation, and the latter was higher than the former with statistically significant(χ2 =5. 989,P=0. 014). Conclusions Compared with open debridement necrosectomy, the abdominal infection rate, digestive tract fistula rate and mortality of open surgical drainage were all significantly reduced , which may be a better treatment for WOPN.
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Objective To explore the significance of gene promoter methylation of CDH1 in breast invasive ductal carcinoma.Methods The gene promoter methylation of CDH1 and E-cadherin expression status in breast cancer tissues,adjacent tissues and normal breast tissues were detected by using methylation specific polymerase chain reaction (PCR) and immunohistochemistry (SP) method.The clinicopathological data (genetic background,age,tumor size,axillary lymph node metastasis,tumor cells grading,clinical staging and molecular subtype) were collected,and analysed the clinical significance of gene promoter methylation of CDH1 in breast cancer.Results Among the 250 patients with breast cancer,113 cases were found gene promoter methylation of CDH1,and the methylation rate was 45.20％.Compared with patients with unmethylated CDH1 gene promoter,the E-cadherin protein expression was reduced in patients with methylated CDH1 gene promoter,there was statistically significant difference (x2 =21.360,P＜0.01).The univariate analysis showed that statistically significant differences were found in axillary lymph node metastasis (x2=19.086,P＜0.01),histological grading of tumor (x2 =8.487,P=0.014),CerbB-2 expression (x2=9.475,P=0.002) and molecular typing (x2 =25.482,P＜0.01) between patients with methylated and unmethylated CDH1 gene promoter.The COX regression analysis showed that there was significant difference in 5-year survival rate between patients with methylated and unmethylated CDH1 gene promoter(P＜0.01).Conclusion Methylation of CDH1 gene promoter causes decreasedexpression of mRNA,and is associated with axillary lymph node metastasis in breast cancer,which suggests that methylation of CDH1 gene promoter plays a certain role in breast cancer progression.
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Objective To explore the pathological characteristics and prognostic influencing factors of T1 invasive ductal breast carcinoma with calcification.Methods The clinicopathological and follow-up data in 172 patients with initially treated operable T1 invasive ductal breast cancer in this hospital from June 2012 to June 2013 were analyzed restrospectively.The patients were divided into the calcification group and non-calcification group based on the breast X-ray image features.The differences of pathological characteristics between two groups,related factors,and relationship between the calcification expression with patient survival were analyzed.Results The pathological types,lymph node metastasis,Her-2 overexpression,TNM stage and Ki-67 had statistically significant difference between the calcification group and non-calcification group(P＜0.05).The multivariate analysis showed that the cases type,lymph node metastasis and Ki-67 were the related risk factors affecting the calcification expression(P＜0.05).The 3-year disease-free survival rate in the the calcifi cation group and non-calcification group were 87.30％ and 95.06％ respectively.The lymph node status and calcification were the independent predictive risk factors affecting the disease-free survival time of invasive ductal breast carcinoma(P＜0.05).Conclusion Calcification is visible X-ray risk factor of T1 invasive ductal breast carcinoma prognosis.
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Objective To explore the clinical effect of non-invasive positive pressure ventilation(NPPV)for treatment of acute left heart failure after mitral valve replacement. Methods Sixty patients with acute left heart failure after mitral valve replacement in Xinxiang Central Hospital from April 2009 to August 2017 were selected. The patients were divided into control group and NPPV group,with 30 patients in each group. The patients in the control group were treated with double oxygen ab-sorption (mask and nasal catheter),strong heart,diuresis and dilated blood vessels. Based on the treatment of control group, the patients in NPPV group were treated with NPPV therapy. The plasma N-terminal pro-B-type natriuretic peptide(NT-proB-NP)level of patients in the two groups was monitored by rapid determination of immunofluorescence before treatment and 6,24 hours after treatment. The respiratory frequency,blood oxygen saturation,heart rate and oxygen partial pressure monitoring of patients in the two groups was monitored before treatment and 2,6 and 24 hours after treatment. Results The total effective rate of patients in the control group and NPPV group was 92. 4％(26 / 28)and 96. 6％(28 / 29)respectively;there was no sig-nificant difference in the total effective rate between the two groups(χ2 = 1. 25,P > 0. 05). There was no significant difference in the plasma NT-proBNP level between the two groups before treatment (P > 0. 05);the level of NT-proBNP at 6,24 h after treatment was significantly lower than that before treatment in the two groups (P < 0. 05);the level of NT-proBNP of patients in the NPPV group was significantly lower than that in the control group at 6,24 h after treatment (P < 0. 05). There was no significant difference in the respiratory frequency,blood oxygen saturation,heart rate and oxygen partial pressure between the two groups before treatment(P > 0. 05). Compared with before treatment,the respiratory frequency and heart rate of patients were decreased and the blood oxygen saturation,oxygen partial pressure were increased at 2,6,24 h after treatment in the two groups (P < 0. 05). There was no significant difference in the oxygen partial pressure between the two groups at 2 h after treat-ment(P > 0. 05);the oxygen partial pressure of patients in the NPPV group was significantly higher than that in the control group at 6,24 h after treatment(P < 0. 05);there was no significant difference in the respiratory frequency,blood oxygen satu-ration and heart rate between the two groups at each time piont after treatment(P > 0. 05). Conclusion NPPV is an effective treatment for acute left heart failure after mitral valve replacement.
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<p><b>PURPOSE</b>RNA helicase p68 plays an important role in organ development and maturation through tuning cell proliferation. However, the character and role of p68 in the whole wound healing process need more study.</p><p><b>METHODS</b>First, we characterize expression of p68 in normal rat skin development postnatal. Then, we assayed dynamic change of p68 in rat skin from different stage after injury, and explored the role of p68 in proliferation and migration of three types of wound healing related cells.</p><p><b>RESULTS</b>p68 was down-regulated during skin developmental and maturation process, up-regulated after wound, peaked on day 14 and then significantly decreased. Wound fluid enhanced wound healing related cell proliferation and up-regulated expression of p68. Conversely, reducing p68 expression by RNA interference resulted in significantly slower proliferation and migration.</p><p><b>CONCLUSION</b>Our results define an important role of RNA helicase p68 in skin wound healing process.</p>
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The system of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated nuclease (Cas) 9 is an effective tool for revising the genome with great accuracy,and boost the advances in life science.By employing this system,we discover the regulation role of key gene during retina development and correct the abnormal mutation of these genes.In this paper,we summarize CRISPR-based technologies that enable mammalian genome editing and their various applications.And CRISPR/Cas9 may be a promising tool to disclosure the mechanism of retinal diseases so as to develop novel treatment for patients with retinitis pigmentosa.
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The system of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated nuclease (Cas) 9 is an effective tool for revising the genome with great accuracy,and boost the advances in life science.By employing this system,we discover the regulation role of key gene during retina development and correct the abnormal mutation of these genes.In this paper,we summarize CRISPR-based technologies that enable mammalian genome editing and their various applications.And CRISPR/Cas9 may be a promising tool to disclosure the mechanism of retinal diseases so as to develop novel treatment for patients with retinitis pigmentosa.
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Obiective To investigate the association between single nucleotide polymorphisms (SNPs) of ER β gene and susceptibility of breast cancer in Uygur women in Xinjiang.Methods A case-control study was designed to explore the genotypes of Rsa Ⅰ (G/A) of ER β gene,detected by PCR-restriction fragment length polymorphism (PCR-RFLP) assay,in 112 breast cancer cases of Uygur women and 139 medical health cases of Uygur women.The association between SNPs of ER β gene and risk of breast cancer in Uygur women was analyzed by unconditional Logistic regression model.Results The frequencies of genotypes of Rsa Ⅰ (G/A) of ER β gene in cancer group and control group were 83.0 ％ and 17.0 ％,73.4 ％ and 26.6 ％,respectively.Rsa Ⅰ (G/A) locus allele frequency were 91.5 ％ and 8.5 ％,86.7 ％ and 13.3 ％,respectively.There were no statistically differences between the cancer cases and control cases (x2 =3.335,P =0.068.x2 =2.917,P =0.088).Presence of estrogen exposure history of two groups for genotypes distribution were 74.2 ％ and 25.8 ％,86.4 ％ and 13.6 ％,respectively.Any family history of cancer in the two groups for the genotypes distribution were 100 ％ and 0,72.8 ％ and 27.2 ％ respectively.There were statistically significant difference between two groups (P =0.046,P =0.001).Compared with wild-type genotype GG,the GA type with estrogen exposure and without a family history of cancer showed a lower incidence of breast cancer in Uygur women (OR =0.385,95 ％ CI 0.148-0.999.OR =0.285,95 ％ CI 0.134-0.605).Conclusions ER β gene SNP is associated with breast cancer of estrogen exposure and no family history of cancer factors.GA genotype may be a protective factor for Uygur women with breast cancer.
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To study the constituents of Rubus corchorifolius L. f, the constituents were separated by using various chromatographies and the structures were identified on the basis of chemical and spectral analysis. Two compounds were isolated from Rubus corchorifolius L. f. Their structures were identified as ent-kauran-3beta, 16beta, 17, 19-tetrol (1), ent-2-carbonyl-16beta-hydroxy-kauran-17-beta-D-glucoside (2). Compounds 1 and 2 are new compounds.
Subject(s)
Diterpenes, Kaurane , Chemistry , Glucosides , Chemistry , Molecular Structure , Plant Leaves , Chemistry , Plants, Medicinal , Chemistry , Rosaceae , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Dihydromyricetin (DMY) on antilipid-peroxidation.</p><p><b>METHOD</b>The antilipid-peroxidation of DMY on heart, liver, brain tissue homogenate and mitochondria was measured by the determination of malondiadehyde (MDA) induced by Fe2+ -Vit C, Fe2+ -H2O2, Fe-Cys with TBA spectrometric method.</p><p><b>RESULT</b>DMY could inhibit the lipid peroxidation of homogenate and mitochondria. The inhibition exhibited concentration-dependent manner.</p><p><b>CONCLUSION</b>DMY has good antilipid-peroxidation effect, which is worth studing further.</p>
Subject(s)
Animals , Male , Rats , Ampelopsis , Chemistry , Antioxidants , Pharmacology , Brain , Metabolism , Dose-Response Relationship, Drug , Flavonols , Pharmacology , Lipid Peroxidation , Liver , Metabolism , Malondialdehyde , Metabolism , Mitochondria , Metabolism , Mitochondrial Swelling , Myocardium , Metabolism , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study chemical constituents of Ilex kudingcha.</p><p><b>METHOD</b>The constituents were isolated by chromatographic method and the structures were identified on the basis of spectral analysis.</p><p><b>RESULT</b>Seven compounds, lupeol(I); 3 beta-hydroxyl-lup-20(29)-ene-24-methyl ester(II); lup-20(29)-ene-3 beta-, 24-diol(III); beta-sitosterol(IV); ursolic acid(V); daucosterol (VI); mannitol were obtained(VII).</p><p><b>CONCLUSION</b>I, III, VI and VII compounds were obtained from genus Ilex for the first time.</p>
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Ilex , Chemistry , Mannitol , Chemistry , Pentacyclic Triterpenes , Plant Leaves , Chemistry , Plants, Medicinal , Chemistry , Sitosterols , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>AIM AND METHODS</b>The relation between AT sclerosis (loss of neurons and proliferation of astrocytes) and long-lasting epileptic susceptibility was investigated by thionine staining, GFAP immunohistochemistry and observing the behavior of rats, after scorpion venom (SV) or normal saline (NS) administrated for three week.</p><p><b>RESULTS</b>Compared with NS+ NS group, both the loss of neurons and proliferation of astrocytes were very marked in KA+ NS group (epileptic susceptible rats) (P < 0.05), but those changes were not visible in KA+ NS group (epileptic nonsusceptible rats).</p><p><b>CONCLUSIONS</b>It suggested that AT sclerosis may be one of important reasons of the long-lasting epileptic susceptibility.</p>
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Animals , Male , Rats , Astrocytes , Pathology , Epilepsy , Pathology , Glial Fibrillary Acidic Protein , Metabolism , Neurons , Metabolism , Pathology , Rats, Sprague-DawleyABSTRACT
Objective To investigate the expression change of P2X3 receptor in spinal dorsal horn and dorsal root ganglion(DRG) in rats with experimental inflammatory pain.Methods Totally 30 SD rats received a subcutaneous injection of carrageenan in the right paw to establish inflammatory pain model,and then divided randomly and equally into 5 groups,that is 6,12,24,72 and 168 h after injection.Another 6 rats undergoing a subcutaneous injection of normal saline in the right paw served as control.Pain threshold was assayed by thermal radiation and von Frey filament in 2,4,6,12,24,72 and 168 h after injection.Expression of P2X3 receptor in spinal dorsal horn and DRG of every group was measured by immunohistochemical staining(IHC).Results After injection of carrageenan,thermal hyperalgesia and mechanical allodynia appeared in the rats.The thermal withdrawl latency(TWL) and 50% paw withdrawl threshold(PWT) were decreased obviously from 2 h,descended to the lowest at 12 h,then returned to the normal level after 72 h.The expression of P2X3 receptor in spinal dorsal horn was increased obviously from 24 h,while that at DRG was increased obviously from 12 h.Both of the expressions lasted for 72 h,and returned to the normal level at 168 h after injection.Conclusion Thermal hyperalgesia and mechanical allodynia appear in the rats after carrageenan injection,and the P2X3 receptor is activated and up-regulated in the DRG and spinal cord,suggesting that P2X3 receptor may play an important role in the incidence and development of inflammatory pain.