ABSTRACT
Nude mouse bearing human bladder cancer exhibits similar growth characteristics as human bladder cancer and therfore is a good simulation model in the research of the biological behaviors of human bladder cancer. To explore the initiation, development, prognosis and biological behavior of human bladder cancer,and to develop intravesical biological agents and antitumor drugs with improved strategies for prevention and treatment of bladder cancer, it is essential to establish human bladder tumor-bearing nude mice for basic and clinical researches. This is a review of the current status of the application of human bladder tumor-bearing nude mice.
ABSTRACT
Objective To investigate the immune localization of bispecific gene antibody (anti human bladder carcinoma/anti VEGF),and to determine whether bispecific gene antibody can inhibit growth of human experimental bladder carcinoma. Methods Immunohistochemistry and immune electron microscope were used to study the immune localization in 60 samples of bladder cancer (experiment group),and 20 samples from BPH cases served as control group.Bispecific antibody was injected into the sites in nude mice,which was adjacent to the xenograft tumor of human bladder transitional cell carcinoma.The tumor size was measured at different times.The microvascular density(MVD) and apoptotic index(AI) in the tumors were examined. Results Of the 60 bladder cancer samples 53 (88.3%) were positive for antigen,while only 1 (5.0%) of the 20 in control group was positive.Two weeks after treatment,the tumor size of experiment group was ( 21.47 ?6.57)mm 2,while that of control group was (59.85?15.43)mm 2.MVD of experiment group was 2148?109,while that of control group was 4056?367.AI of experiment group was 17.26,while that of control group was 7.09.The differences between the 2 groups were significant (each P
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Objective:To analyse and prepare bispecific antibody(BsAb, anti human bladder cancer/anti VEGF) which could enhance the effectiveness of tumor cell killing activity.Methods:Monoclonal antibodies against human bladder cancer and VEGF were used to prepare bispecific antibody.Results:IC50 of BsAb was 10 -9.5 ,while those of antibodies against human bladder cancer and VEGF were 10 -8.9 and 10 -8.3 .Conclusion:The effectiveness of BsAb was significantly higher than that of monoclonal antibodies. [
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Objective:To study the inhibitory role of BsAb(anti-human bladder cancer/anti-VEGF) on human bladder cancer growth.Methods:BsAb was used to treat animal models of BIU-87 cell lines.The tumor inhibition rate was calculated and the tumor microvessel density was detected by immunohistochemical techniques.Results:BsAb could significantly inhibit growth of tumor.And the tumor microvessel density was lower than that of the control group.Conclusion:BsAb could inhibit tumor growth by inhibiting angiogenesis. BsAb may become a new method for human bladder cancer treatment.
ABSTRACT
Objective:To study vascular endothelial growth factor receptor (VEGFR) in bladder carcinoma and its relationship with clinical pathology.Methods:VEGFR of bladder carcinoma samples (50 cases) and normal bladder tissues (20 cases) was detected with immunohistochemistry which was streptomyces-antibiotin-peroxidase-linkage-Technique (Technique SP).Results:VEGFR was positive in most of bladder carcinoma cases.Percentage was 74%.Expression level had positive relationship with pathologicl level and tissue grade.Conclusion:VEGFR was positive in bladder carcinoma.It indicated that VEGFR had important function in angiogenesis of bladder carcinoma. [