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Cryopreservation, the most popular way to preserve human sperm, led to a significant decline in sperm motility. Here, we tried to introduce a new method to store sperm without freezing. Different concentrations of genistein were added to liquid preserved sperm. We investigated the effects of supplementation on sperm total antioxidative capacity (T-AOC), glutathione(GSH), methane dicarboxylic aldehyde (MDA), acrosomal enzyme activity, and fertilization ability of sperm. The effects of liquid storage and cryopreservation on sperm parameters were also compared. IVF medium supplemented with genistein (20µmol L-1 ) maintained sperm motility for up to 11 days. The addition of genistein led to a decrease in reactive oxygen species (ROS) generation that demonstrated an effective improvement in sperm motility and decreased the MDA production and maintained the GSH content and enhanced the oxidative stress resistance ability of the sperm during liquid storage. The storage sperm were used for intracytoplasmic sperm injection(ICSI) into human oocytes and activated oocytes successfully. Sperm stored in liquid medium containing genistein was superior to sperm stored in liquid nitrogen in terms of antioxidant stress and fertilization ability. We confirmed that genistein could be used as an antioxidant for the liquid storage of sperm. Sperm stored in an IVF medium with genistein could avoid cryodamage, which may become an alternative option in assisted reproduction technology. (Afr J Reprod Health 2022; 26[7]: 72-82)
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Humans , Male , Sperm Motility , Antioxidants , Semen Preservation , Fusion Proteins, gag-onc , Reproductive Techniques, AssistedABSTRACT
Body donation is the main source of human specimens preparation in medical universities and colleges. The low donation rate in China restricts the improvement of basic medical research and clinical treatment seriously. In the investigation, deep-rooted traditional concepts, unsound legal system, and inadequate government propaganda have become the major factors influencing body donation. Combined with the practice of body donation in the localities, this study puts forward some measures to promote the body donation, such as giving targeted publicity, promoting the concept of civilized funeral and burial, strengthening legislation, and simplifying the body donation process, which would lay the foundation for the development of medical education and research.
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Objective:To Analyze the risk factors and prognosis of patients with hyperfibrinolysis after severe trauma.Methods:A prospective case-control study was conducted to analyze the clinical data of severe trauma patients whose injury severity score (ISS)≥16 points admitted to First People's Hospital of Taicang from October 2017 to December 2018. Blood clot dissolution rate parameter (LY30) ≥3% was diagnosed as hyperfibrinolysis. The patients were divided into hyperfibrinolysis group ( n=27) and non-hyperfibrinolysis group ( n=27). Data were collected including patients' general information, hemoglobin (Hb), hematocrit (Hct), platelet count (PLT), fibrinogen (Fg), lactic acid value and thrombelastograghy (TEG). Univariate and multivariate Logistic regression analysis were used to identify risk factors of hyperfibrinolysis after severe trauma. Prognostic indicators and K-M survival curve of the patients were analyzed. Results:(1) A total of 142 patients with severe trauma were enrolled. There were 101 males and 41 females, aged 45-65 years (mean, 56 years). The incidence of hyperfibrinolysis was 14.8%. (2) Univariate analysis showed that ISS, abbreviated injury scale (AIS) ratio of pelvic limbs ≥3 points, shock index (SI) ratio >1, temperature, PLT, Fg and lactic acid value were correlated with the occurrence of hyperfibrinolysis after severe trauma ( P<0.05). (3) Multivariate Logistic regression analysis showed that PLT ( OR=1.035), ISS ( OR=0.898) and lactate acid value ( OR=0.735) were independent risk factors for hyperfibrinolysis after severe trauma ( P<0.05 or 0.01). (4) Hyperfibrinolysis group required more massive transfusion (38.1% vs. 2.5%), were more prone to multiple organ dysfunction syndrome (MODS) (90.5% vs. 48.8%), and had a higher 24-hour mortality (47.6% vs. 8.3%), when compared with non-hyperfibrinolysis group ( P<0.01). K-M survival curve indicated that the median survival time in hyperfibrinolysis group after trauma was 1.33 days ( IQR, 0.40-17.20 days). Conclusions:The incidence of hyperfibrinolysis after severe trauma is low. ISS, PLT and lactic acid value are the independent risk factors for hyperfibrinolysis after severe trauma. Patients with severe post-traumatic hyperfibrinolysis are characterized by high probabilities of massive transfusion and MODS, short survival time and high early mortality.
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First-period examination in empirical study of clinical medical national licensing staged examination has been implemented for two years in Binzhou Medical University (BZMU).During the examination,BZMU obtained the experience of organization,guarantee,and training,which adopted corresponding reform measures including course teaching,assessment method,incentive reward and punishment,etc.The first-period examination of staged examination is national examination of quality of education and teaching on the university,which has promoting effect to strengthen the core of the education teaching and renew the idea of teachers' teaching evaluation.It can improve the evaluation system of curriculum assessment and promote students correctly using the theoretical knowledge to clinical practice skills and improve alignment between the university and hospital.Finally,it can promote the reform and development of medical education.
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Aiming at the problems faced by the medical postgraduate students in the cooperative medical education and the classified training mode,combined with the characteristics of the cultivation of medical postgraduates,the article constructed the quality guarantee system of graduate education based on project management.From the three dimensions of time dimension,corpus dimension and objective dimension,the article established a model of training quality guarantee system and analyzed the four work modules:construction of project organization,standardization of whole life cycle process,control of project quality and integration of project information.In addition,the article introduced the practice of this quality guarantee system in Binzhou Medical University.
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The occupation post exposure prophylaxis for AIDS has been carried out for many years and achieved remarkable result,but local trials of non-occupational exposure prophylaxis encountered many ethical problems and difficulties.In the context of high willingness in the AIDS high-risk population to accept non occupational exposure prophylaxis and increasing demand for drugs,starting from the value concept of life dignity,based on the ethical principles of good without hurt,fairness and justice,it is recommended to strengthen the propaganda of non occupational exposure prophylaxis and improve the availability of prophylaxis drug for non occupational exposure,in order to enable the high-risk groups possibly infected with HIV received timely preventive treatment.
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Objective To investigate the clinical value of immunophenotyping and quantitative analysis of serum immunoglobulin in the diagnosis and typing of monoclonal gammaglobulinemia.Methods 118 serum samples were collected from the patients with monoclonal gammaglobulinemia and performed the serum protein electrophoresis(SPE),serum immunofixation(IF)electrophoresis, immunoglobulins(Ig)quantitation and serum light chain κ,λdetection.The analysis was conducted aiming at the patients with im-munophenotyping positive and the Ig quantitation negative.Results 56 cases showed immunophenotyping positive and the immuno-globulin quantitation negative,among them,6 cases were free light chain type (κlight chain in 2 cases andλlight chain in 4 cases), 1 case was the non-secretion type;62 cases showed immunophenotyping positive and the Ig quantitation positive.Conclusion The IF technique has an important significance for the diagnosis and typing of monoclonal gammaglobulinemia,its diagnosis can be com-bined with the other laboratory tests of serum Ig and light chain quantitative detection.
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Objective To evaluate the effects of cilostazol on plasma platelet-related indicators in trauma fractures patients with postoperative venous thrombosis.Methods 104 cases of trauma fractures patients with postoperative venous thrombosis from February 2012 to July 2013 in Laiwu City People’s Hospital were collected and randomly divided into group A and group group B,each had 52 cases Two groups were given conventional nursing, and group A were added cilostaeol group B were added aspirin.Other 50 cases of current healthy people for medical examination were selected for controls (group C).The effects of cilostazol on plasma platelet-related indicators in each treatment group were analyzed. Result Before treatment,the levels of IL-6 and IL-8 in group A and B were significantly higher than group C(P<0.01),but there is no difference between group A and B.The plasma CRP, FIB and DD in group A and B were significantly decreased than group C(P<0.01,P<0.05).After appropriate treatment,the levels of CRP,FIB and DD in group A and B were significantly reduced than pre-treatment(P<0.01,P<0.05).CRP content in group A was lower than that in group B,the difference was statistically significant(P<0.05 ),FIB and DD in group A showed no differencewith group B.Before treatment,PDW,PLT,MPV and P-LCR in group A and B were significantly higher than group C(P<0.01,P<0.05).After appropriate treatment,the levels of PDW,PLT,MPV and P-LCR in two groups were significantly decreased (P <0.01,P <0.05 ),but there was no difference between two groups.Before treatment,the expression of platelet activation in group A,B were significantly higher than group C(P<0.01),but significantly reduced after appropriate treatment (P<0.01),and the difference between the two groups was not statistically significant.Conclusion Cilostazol could reduce the expression of inflammatory factors,levels of D-dimer and fibrinogen and platelet related indicators by reduced platelet activation,which plays antithrombotic effect.
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This study aimed to observe the regularity of liver cell apoptosis and expression of apoptosis related gene Bcl-2 and Bax in the induced cancer, and explore the relationship between apoptosis and the development of liver cancer. In this study, 84 rats were used, 72 rats as experimental group induced by drinking water containing DEN, 12 rats as control group (CG). After laparotomy, the form, color, texture of the liver and metastatic tumor in both control and experimental groups were observed and recorded. The metastatic tumor and the liver tissue were sectioned and stained with hematoxylin-eosin staining to demonstrate the characteristic in morphological changes, the Hoechst 33342 fluorescent staining was applied to show and count the rate of apoptotic cell, and the in situ hybridization technique was used to detect the Bcl-2 and Bax expression. The results showed that the process of carcinogenesis can be divided into three periods: hepatic toxic lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis. The Hoechst 33342 fluorescent staining showed that the rate of apoptosis in the CG, hepatic toxic lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis were 6.7%,18.8%,17.4% and 51.2% c2=33.62, P<0.05 respectively. In situ hybridization showed the yellow positive reaction products of Bcl-2 and Bax located in the nucleus and cytoplasm of cell in diffusive distribution. The average optical densities of Bcl-2 and Bax expression were 0.1697±0.0101 and 0.1383±0.0093 in the control group, 0.1431±0.0072 and 0.1523±0.0103 in hepatic toxic lesion, 0.1261±0.0164 and 0.1639±0.0133 in hepatic proliferation/cirrhosis, 0.1034± 0.0124 and 0.1785±0.0191 in hepatic carcinogenesis, indicating that the expression of Bcl-2 decreased, but that of Bax increased with the development of liver cancer in experimental groups. In DEN induced liver cancer, the origination and development of liver cancer were accompanied with proliferation and abnormal apoptosis, which was related to the abnormal expression of gene Bcl-2 and Bax.
Este estudio tuvo como objetivo observar la regularidad de la apoptosis en células del hígado y la expresión de apoptosis relacionada con los genes Bcl-2 y Bax en el cáncer inducido, además de explorar la relación entre apoptosis y desarrollo de cáncer hepático. Fueron utilizadas 84 ratas, 72 como grupo experimental inducido con el consumo de agua conteniendo DEN, y 12 como grupo de control (CG). Después de la laparotomía, la forma, color, textura del hígado, y del tumor metastásico fueron observadas y registradas en ambos grupos. El tumor metastásico y el tejido hepático fueron seccionados y teñidos con H-E para demostrar la característica de los cambios morfológicos; se aplicó la tinción fluorescente Hoechst 33342 para mostrar y contar la frecuencia de las células apoptóticas, y se utilizó la técnica de hibridación in situ para detectar la expresión de Bcl-2 y Bax. Los resultados mostraron que el proceso de carcinogénesis se puede dividir en tres etapas: lesión hepática tóxica, proliferación hepática/cirrosis hepática y carcinogénesis. La tinción fluorescente Hoechst 33342 mostró que la tasa de apoptosis en el CG, lesión hepática tóxica, proliferación hepática/cirrosis hepática y carcinogénesis fue de 6,7%, 18,8%, 17,4% y 51,2% (c2 = 33,62, P <0,05), respectivamente. La hibridación in situ mostró reacción positiva de productos amarillos de Bcl-2 y Bax en el núcleo y citoplasma de la célula en distribución difusa. Las densidades ópticas medias de la expresión de Bcl-2 y Bax fueron 0,1697±0,0101 y 0,1383±0,0093 en el grupo de control, 0,1431±0,0072 y 0,1523±0,0103 en lesión hepática tóxica, 0,1261±0,0164 y 0,1639 ± 0,0133 en la proliferación hepática/cirrosis y, 0,1034±0,0124 y 0,1785±0,0191 en la carcinogénesis hepática, lo que indica que la expresión de Bcl-2 se redujo, pero que el Bax aumentó con el desarrollo de cáncer de hígado en los grupos experimentales. En el cáncer hepático inducido por DEN, el origen y desarrollo de cáncer hepático fueron acompañados con proliferación anormal y apoptosis, las cuales se relacionaron con la expresión anormal de los genes Bcl-2 y Bax.
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Animals , Rats , Carcinoma, Hepatocellular/pathology , bcl-2-Associated X Protein/metabolism , Liver Neoplasms/pathology , Staining and Labeling , Rats, Wistar , Apoptosis , Carcinoma, Hepatocellular/metabolism , Carcinogenesis , Liver Neoplasms/metabolism , Microscopy, FluorescenceABSTRACT
ObjectiveTo explore the risk factors for upper gastrointestinal bleeding after craniocerebral trauma.MethodsTo retrospectively summarize the clinical features of 80 cases with craniocerebral truma and to analyze the risk factors for upper gastrointestinal bleeding.Results Upper gastrointestinal bleeding was present in 39 of the 80 cases(48.8％) .Twenty-one of the 39 cases with gastrointestinal bleeding died(53.8％)In the patients without gastrointestinal bleeding, 5 cases died (19.2％) .Among the factors affecting the gastrointestinal bleeding after craniocerebral trauma, Glasgow Coma Scale(GCS) score, septicemia, decerebration,hypotention, metabolic acidosis and hyperglucose were closely related risk factors(x2 = 13.96;x2 = 27.43;x2 =46.28 ;x2 = 27.33 ;x2 = 11.88 ;x2 =5.71 ,P ＜0.05 or P ＜0.01).ConclusionProphylaxis against related risk factors could reduce the incidence of gastrointestinal bleeding, improve the prognosis and decrease the mortality of craniocerebral trauma.
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[Objective] To study the effect of vascular endothelial growth factor(VEGF)on proliferation of human bone marrow derived mesenchymal stem cells(h-MSCs)and its chondrogenic differenciation and to provide a basis for the tissue engineering technology of articular cartilage.[Method]The hMSCs were cultured in vitro as usual and its passage was induced the by medium containing 800 ng/ml or 1600 ng/ml the VEGF MSCs the cultured in the medium containing 10 ng/ml the TGF ?-1 which the had multiple the biological efficacies the to enhance the proliferation the of MSCs the and to the inhibit the activities of the inflammatory factors,acted as a positive control.The induced MSCs were used to observe its proliferation and differenciation.The synthesis of chondrocyte extracellular matrix in different induce-factor was measured by immunohisto-chemical staining.[Result]MSCs cultured wit different induced factors proliferated well,MSCs induced by VEGF medium showed a well growth activity and chondrogenic differenciation,but MSCs cultured in TGF?1-containing medium induced more chondrocyte matrix than that in VEGF medium.[Conclusion]VEGF induces chondrogenic differentiation of MSCs in vitro,but MSCs cultured in TGF?1 medium induces more chondrocyte matrix.It is suggested that VEGF acts as donor of inducer message cartilage repair by MSCs.
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Aim To investigate antiaging effects of beta-carotene(?-C) from dunaliella salina on fruit flies and rats and to probe possible mechanisms.Methods The whole study contains fruit flies experiment and rats experiment.In the fruit flies experiment,fruit flies were divided into nine groups according to different ingredient in the nutrient medium.We took life span,flight capability,the number of copulatory fruit flies aged 8 days,and head MDA content of fruit flies aged 40 days as indexes of aging.In rats experiment, 54 aged male rats were divided randomly into 6 groups and bred for 45 days with different diet.Plasma MDA,CAT and GSH-Px were detected.MDA,SOD and GSH-Px in heart,liver,pulmo,lien and brain were also detected.Results Compared with control group in fruit flies experiment,the shortest life span,maximum life span and mean life span were increased in each experimental group.Flight rate and flight frequency were increased,the number of copulatory fruit flies was also increased.The content of MDA was significantly decreased.In rats experiment,plasma MDA content was significantly decreased.The activity of GSH-Px was elevated.Similar results were obtained in different tissues.MDA content was significantly decreased in each tissue sample,while activities of SOD and GSH-Px were significantly inceased.Conclusion ?-C from dunaliella salina had antiaging effects on fruit flies and aged rats.The possible mechanism resided in its antioxidative effects owing to the elevated antioxidases.
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Objective To investigate Hysterothylacium aduncum (Anisakidae) infection in marine fishes from Bohai Sea and Yellow Sea. Methods Nematodes were collected from the digestive tract of fishes, fixed with hot 4% formalin and preserved in 70% ethanol for study. The specimens were cleared in lactophenol for light microscopical examination, and properly treated for scanning electron microscopy (SEM). Results Among the fishes examined, 14 out of 93 species (15.1%) were found infected by H.aduncum, with a higher prevalence in the fish of Lophius litulon(66.7%), Scomber-omorus niphonius(47.5%), and Gadus macrocephalus(33.3%). H.aduncum infection was first recorded in elasmobranch-Raja smirnovi. Morphological differences of H.aduncum were observed, including the width of lateral alae and the length of intestinal caecum. Conclusion H.aduncum in fishes of Bohai Sea and Yellow Sea in China may be a complex species, and its high prevalence in some fishes reminds the risk of anisakiasis by eating raw fishes.