Inhibitory effect of zinc, copper and selenium on telomerase activity in tumor tissues and t47d breast cancer cell line

The present study was performed to investigate the effects of trace elements particularity Se, Zn and Cu on tumor genesis in breast cancer. The inhibitory effect of Se, Zn and Cu, on telomerase activity was analyzed in human breast tumor tissues and breast cancer [T47D] cells. Tissue specimens from 24 women with benign breast disease and 32 women with breast cancer specimens [ductal carcinoma, lobular carcinoma] were collected during surgery. In addition venous blood samples were obtained for assessing the trace elements. T47D cell line was cultured and treated with trace elements. Telomerase activity then was measured with TRAP assay in cell line and tissue extracts. There was a significant difference between tissue and serum levels of Cu, Se and the ratio of Cu/Zn in patients and controls [P<0.001]. After treating with 100 microm/L Zn So4, 10 um /L Cu So4 for 6 hours, telomerase activity of T47D cells was markedly increased. But after treating with 10, and 30 um /L selenium-L- methionin, telomerase activity was markedly inhibited. Telomerase activity of T47D cells for 24 hours were 0.93, 0.60 and for 48 hours were 0.76, 0.12 respectively [control 49.2%]. There were variations in serum level of Zn and Cu in breast cancer patients. Association between trace elements level and telomerase activity level can be exploited as prognostic and diagnostic marker for breast cancer

Variation of adiponectin levels in normal and obese subjects: possible correlation with lipid profiles

Data available suggests that Adiponectin, an adipocyte-derived peptide, is associated with adiposity and could effect the regulation of glucose and lipid metabolism in humans. The aim of this study was to evaluate the association between serum adiponectin concentrations and anthropometric indices and lipid profiles among Iranian women with different grades of obesity. In this analytical descriptive study of 157 non-diabetic women [33 normal weight, BMI< 25 kg/m2 and 124 overweight and obese, BMI >/= 25kg/m2], serum adiponectin and leptin levels were measured using an enzyme-linked immunoassay. Fasting glucose and lipid profile levels determined by the glucose oxidize and enzymatic methods, respectively. Mean serum adiponectin concentration significantly decreased with obesity [p<0.05]. Although adiponectin showed a significant negative correlation with BMI [r=-0.321], it was correlated with serum leptin [r=-0.139], glucose [r=0.259], LDL-C [r=-0.125], TGs [r=-0.210] levels, TSF [r=-0.145], WHR [r=-0.159], and positively with serum HDL-C concentration [r=0.218] in all subjects [p<0.05]. Results of multiple regression analyses showed that adiponectin as a dependent variable had a significant correlation with BMI [beta =-0.605, P=0.017], waist circumference [beta =0.624, p=0.029], WHR [beta =-0.251, p=0.048], frame [beta =0.260, p=0.018], TC/HDL-C ratio [beta =-0.1.309, p=0.040] and LDL/HDL ratio [beta =-1.343, p=0.007] and changes in waist size had a significant effect on serum adiponectin levels. Our results suggested that adiponectin had an inverse correlation with adiposity indices and unfavorable lipid profiles, and that variation of waist circumference mostly affected Iranian women

[Correlation between leptin serum levels with lipid profile and anthropometric indices in women with different grades of obesity]

Background and Objective: leptin is a 16 KDa peptide which has a close correlation with adiposity. However, its effect on lipid profile is controversial in human. Therefore, this study was performed to investigate correlation between variations in serum leptin levels with lipid profile and anthropometric indices in women with different grades of obesity

Materials and Methods: the current cross-sectional study was carried out on 149 healthy non diabetic women, including 33 normal weight [BMI<24.9 kg/m2] and 116 women with different grades of obesity [BMI>25 kg/m2] with age range of 15-49 years, respectively. Serum levels of leptin, fasting blood glucose and lipid profile [total cholesterol, triglyceride and HDL-cholesterol] were measured using high sensitive immunoassay, glucose oxidase and enzymatic methods, respectively

Results: mean serum leptin levels were 15.34, 32.78, 42.13, 43.22 and 45.23 ng/ml in normal, overweight, obese grade I, obese grade II, and obese grade III women, respectively. Difference in mean of leptin serum level, lipid profile, and anthropometric indices was statistically significant between different groups [p<0.001]. Leptin Serum levels had significant correlation with BMI [p<0.05, r=0.623]. In addition, it showed a direct significant correlation with levels of fasting blood glucose [r=0.297] and lipid profile [total cholesterol [r=0.347], triglyceride [r=0.428], and LDL-cholesterol [0.367]] [p<0.05]. In contrast, it showed an indirect correlation with HDL-C serum levels [r= -0.320, p<0.05]

Conclusion: results of the current study showed that leptin serum level has a close direct correlation with adiposity indices and lipid profile and its level increases significantly with increasing grades of obesity