[Repeatabilifi of ctinico-histopathologiculft.ndings and direct immanoflaotescence offtxed, paraffin embedded specimens fot diugnosis of lichen planas]

Background and. Aim: Lichen planus [LP] is a chronic mucocutaneous disorder mediated by the impaired immunity' in which the concomitance of clinical and histopathological findings are necessary for a definite diagnosis. In case of discrepancy between clinical and histopathological findings, use of adjunct diagnostic methods such as direct immunofluorescence [DIF] is recommended. This study sought to assess the repeatability of clinico histopathological findings and direct immunofluorescence of fixed, paraffin embedded specimens for diagnosis of LP.

Matertuh and Methods: In this retrospective descriptive study, 49 oral and cutaneous LP specimens were subjected to DIF assessment using fibrinogen, c3 and IgM. The intensity of fluorescence staining was graded as 0, I or 2' Findings were compared in each group and between oral and cutaneous LP groups using statistical tests.

Results: Statistical unulyr",, "rr"aled a correlation between C3 and IgM expression [Spearman's rho: 0'697, p=0'000]' which was more prominent in cutaneous specimens [p:0.000]. Additionally, a correlation was observed between fibrinogen and IgM expression in oral LP [Spearman's rho: 0.764, p:0.02].

Conclusion:It seems that DIF assessment of formalin-fixed, paraffrn embedded specimens using C3' fibrinogen and IgM does not have adequate repeatability for clinico-histopathological findings; although, combined application of C3 and IgM in cutaneous uoa IgVt and fibrinogen in oral LP specimens showed higher repeatability.

[Comparison of the expression level of P53 and proliferating cell nuclear antigen [PCNA] genes in the inflamed and non-inflamed dentigerous cysts]

The role of P53 and proliferating cell nuclear antigen [PCNA] genes in inflamed and non-inflamed odontogenic cysts is related to cell proliferation but it is unclear. The aim of this study was immunohistochemical evaluation of P53 and PCNA expression in inflamed and non-inflamed dentigerous cysts. In the present study, 23 dentigerous cysts [12 inflamed and 11 non-inflamed] were surveyed. Three microns slices were got from paraffined blocks and stained with P53 and PCNA antibodies by immunohistochemistry. Slides were seen by oral pathologist with optical microscope [Olympus BX41] in x400 magnification and scored by Allred scoring. Data were analyzed by Mann-Whitney test via SPSS software. Percent of stained cells in inflamed compared non-inflamed dentigerous cyst did not show any significant difference for expression level of P53 and PCNA in basal and suprabasal layers [P> 0.05]. It seems that inflammation does not affect on expression level of P53 and PCNA in dentigerous cyst

[Histopathological effect of advanced periodontal disease on the dental pulp]

Statement of Problem: Many authors have claimed that pulpal inflammation may occur following periodontal diseases. Appropriate diagnosis of different lesions that have affected the dental pulp or periodontium is critical for prevention of unnecessary or harmful treatments; this must be taken into account before treatment

Purpose: The purpose of this study was histological evaluation of the pulp in the teeth with advanced periodontitis

Materials and Method: 30 permanent single teeth root that had advanced periodontitis with attachment loss >/= 5 mm at least in one surface were used. The teeth were not maintainable and did not have caries, restoration and any sign of primary trauma from occlusion and did not receive any periodontal professional treatment in the past 6 months with no background of trauma. After clinical and radiographical examination and confirmation of the existence of advanced periodontitis, the teeth were extracted. Then cracks were created in the teeth by special clips. After fixation of the teeth in 10% formalin solution and decalcification by 10% nitric acid, the sections were prepared and stained by hematoxylin and eosin and then evaluated from histological perspectives. The data were analyzed by Spearman correlation coefficient ANOVA, t-test and Kruskal wallis tests

Results: In this survey, we did not find any significant correlation between clinical findings and histopathological situation. The relationship between clinical attachment loss and pulp diagnosis was statistically significant [p =0.043]. Also there was a statistically significant relationship between clinical attachment loss and calcification in the pulp [p =0.014]

Conclusion: According to the result of this research, it seems that periodontal condition affects the pulpal condition and it should be considered in future treatments on these teeth

[Calcifying odontogenic cyst associated with complex odontoma-a case report]

Calcifying odontogenic cyst [COC] is an uncommon developmental odontogenic cyst first described by Gorlin in 1962.It is named cystic calcifying odontogenic tumor in new classification by WHO. It is considered as extremely rare and accounts for only 1% of jaw cysts reported. It is unusually seen associated with odontoma. The Case was a 15 year-old male patient with the chief complaint of painless swelling in the right side of anterior part of his maxilla. Radiographic pattern was radiolucent with radioopacitis foci. In histopathological view, epithelium of odontogenic cyst with ghost cells and complex odontoma were seen. Treatment was done by enucleation, in 6 months follow up there was not any recurrence. Clinical and radiographic features of odontogenic lesions were non-specific. Always we must careful that the type of lesion associated to Gorlin cyst appoints the clinical behavior, method of treatment and prognosis of combined lesion

[Adenomatoid odontogenic tumor in calcifying odontogenic cyst; a case report]

The term calcifying odontogenic cyst was first used by Gorlin in 1962 to describe a new cystic entity with a peculiar pathogenesis and histologic feature. Other odontogenic tumors such as ameloblastoma, odontoma, amelobalstic fibroma, ameloblastic fibroodontoma, odontoameloblastoma, pindborg's tumor, and adenomatoid odontogenic tumor may seldom be associated with this lesion. This paper describes the case of a 16-year-old male with a remarkable combination of two entities. The lesion associated with an impacted canine tooth was detected at the mandibular left side, producing an extensive expansion of buccal cortical plate on panoramic view. During the operation, enucleation of the cyst was performed and the impacted canine tooth was extracted. The histologic specimen revealed the diagnosis of an adenomatoid odontogenic tumor [AOT] in a calcifying odontogenic cyst [COC]