ABSTRACT
In this study, 120 bovine and ovine serum samples were collected and tested for brucellosis with Rose Bengal test [RBT], modified Rose Bengal test [mRBT], buffered acidified plate test [BAPT] using conventional Rose Bengal antigen [RBA] and buffered acidified plate antigen [BAPA] and antigens prepared from B. melitensis [the main cause of brucellosis in Egypt] strain 16-M. Indirect ELISA was used for testing the same serum samples using 4 different coating antigens which were S-LPS and OMP antigens prepared from 16-M strain and S-LPS and OMP prepared from S19 strain. There were some differences between the results of conventional Rose Bengal and BAPA antigens prepared from B. melitensis 16-M strain. In the same time, there were no characteristic differences in results of Indirect ELISA
Subject(s)
Brucella melitensis/immunology , Agglutination Tests/methods , Enzyme-Linked Immunosorbent Assay/methods , Serologic TestsABSTRACT
The present work was planned to develop a modified system for the growth of C. tetani and production of tetanus toxin, the system includes growth media that contain no meat or dairy by-products using non-animal based products. Hydrolyzed soy proteins have been found to replace the meat/dairy product for growth of C. tetani and the production of its toxin, these replacements resulted in production of high tetanus toxin titer and of low side effects