Immunodetection of amplicaecum larval haemoglobin using ELISA technique

Amplicaecum larvae are a significant parasite infesting tilapia and some predatory fish species at Nasser Lake since 1988. These larval stages were homogenized and prepared as a crude antigen [structural antigen]. The protein concentration was estimated by monitoring the UV absorption spectrum. The positive serum anti Amplicaecum larval hemoglobin was prepared and sensitized in vivo. The highest sensitivity of the hemoglobin was estimated by ELISA technique and amounted to a titer of 1/20 with optical density x 0.554

Comparative analysis of ascaridial parasitic crude antigen by SDS-page and UV absorbance techniques

Ascaridial nematodes are among the well-known parasites infesting fish, birds and mammals. Crude antigen was extracted from Amplicaecum larvae [AL3], Ascaridia galli [adult worm, AG] and Neoascaris vitulorum [adult worm, AV]. The characterization of these parasitic nematodes revealed relative homologous polypeptide chains [MW 25.5 kDa] common to the three parasitic species, whereas the UV absorbance scan mode wave length [WL 200/550 nm] as related to the absorbance [AB] value 0 - 2 recorded an indicative frequency curve. The protein concentration in Amplicaecum, Ascaridia and Neoascaris figured up to 5.47 g%, 4.93 g% and 2.2 g%, respectively

Occupational nail mycoses in a veterinarian

Mycological examination was carried out to assess the etiological agent of nail infection in a Veterinarian occupationally involved in mycological investigations. Scopulariopsis infection of the nail was confirmed macroscopically and microscopically. Successful treatment was achieved by oral administration of ketaconazol and topical application with isoconazol sol