Cytogenetic evaluation of in-vitro matured buffalo oocytes in different culture conditions

Ocytes were collected by aspiration from ovaries of slaughtered buffaloes. A total of 1240 oocytes were selected for in vitro maturation [IVM] procedure in culture media for 22- 24 hr and 25 -28 hr at 39°C in 5% CO[2] incubator. For both cultured periods oocytes were matured in droplets of TCM-199 + estrous buffalo serum [EBS], TCM-199 + foetal calf serum [FCS], Ham's F-10 + EBS and Ham's F-10 + FCS. Cytogenetic analysis of 582 oocytes cultured in the four media conditions in both periods of time showed wide range of meiotic configurations : metaphase-I, anaphase-I, telophase-I and metaphase-11. Also, the cytogenetic analysis identified matured oocytes at metaphase-II with diploid number of chromosomes [3.95%]. Analysis of variance showed that, the maturation rate in the two media and sera didn't differ significantly. The maturation rate to the second metaphase were significantly higher [P0.01] at 25 - 28 hr than in the same media for 22 - 24 hr period of time

Cytogenetic analaysis of early stage buffalo embryos matured and fertilized in vitro

The in vitro fertilization technique offers the opportunity to study the karyotype of gametes and early embryos. Follicular buffalo oocytes were matured in TC-199 medium enriched with FCS, fertilized in vitro using thawed frozen buffalo semen and cultured at 38.5°C, 5% CO[2] and high humidity. Chromosome preparations were made from 232 buffalo embryos at 4 - to 8-cell stage after culturing for 48 hr in vitro. Only 74 embryos from 170 fixed embryos provided analyzable chromosome spreads. The percentage of embryos with chromosomal aberrations was 12.16%. The observed abnormal embryos were 5.41% haploid, 4.05% polyploid [2.70% triploid and 1.35% pentaploid] and 2.70 haploid/ diploid mosaicism. It is concluded that cytogenetic analysis of buffalo embryos is valuable for detection of chromosomal abnormalities which hinder the cleavage processes to reach to blastocyst stage

Residue-concentration of malathion and its metabolites in blood, urine, milk and tissues of lactating goats after multiple oral administration

Determination of Residues of Malathion [M] and its metabolic fractions Malaoxon [MO], 0, O-dimethyl-phosphoro dithionate [DPDT], Malathion mono acid [MCA], Dicarboxylic acid [DCA] and desmethylmalathion [DMM], in serum, urine and tissues of lactating goats received a daily dose [10mg-1 kg] body weight of [M] for 5 successive days was investigated. Daily samples of blood and urine were collected during the period of administration as well as 7 and 14 days after withholding of [M]. Tissue samples were taken from slaughtered goats at 7 and 14 days after cessation of the insecticide. Gas chromatographic technique [GC] was adopted for determination of [M] and its metabolites. [M] was not detected in any serum samples collected during administration and/or after cessation of insecticide, while it was excreted unchanged in urine and milk till one week after cessation of treatment with neurotoxic concentration over 20 ng/ml. The active metabolites [MO] and [DPDT] were not detected in any milk samples collected during application or after stoppage of [M] administration, while both metabolites were detected in serum and urine with neurotoxic concentrations during period of administration and still detected in serum for 7 days [400 ng/ml] and DPDT for 14 days [241 ng/ml] after stopping of [M] administration. The major excretory detoxified metabolites was found in milk on the days 7 and 14 after cessation of Malathion. Tissue residue-analysis

Effect of the seasonal changes on recovery, quality and maturation of buffalo oocytes in vitro

The present work aimed to study the effect of seasonal variations on the recovery rate, quality of recovered oocytes and maturation rate of buffalo oocytes in vitro. The effect of culture period on maturation rate was studied. The follicular oocytes were collected from ovarian follicles [2-5 mm in diameter] of slaughtered buffaloes by aspiration method. The recovered oocytes were classified morphologically into three categories [A, B and C]. The selected recovered oocytes [class A and B] were matured in tissue culture medium-199 supplemented with 10% fetal calf serum and antibiotics at 39°C, 5% Co[2] and high humidity for two different culture periods [21-24 hr and 25-28 hr]. Cultured oocytes were examined for nuclear maturation. The results indicated that, the average number of buffalo oocytes was 3.33 +/- 0.81 oocytes /ovary, without significant variation between the four, seasons. A higher [p<0.001] percentage of high quality oocytes was obtained in winter, autumn, spring than the summer season, while the percentage of poor quality oocyte [class C] was increased significantly [p<0.01] during summer. Moreover, the percentage of oocyte suitable for maturation decreased in summer than other seasons. The maturation rate of oocytes cultured for 21-24 hr was lowered than that recorded after 25-28 hr incubation period, among different four seasons. It is concluded that the quality of buffalo oocytes but not the recovery rate was affected by the seasonal variations. Increasing in the time of culture period was associated with improving in maturation rate. The high quality oocytes and high maturation rate can be obtained all over the year except summer season after culturing for 25-28 hr in vitro

Effects of ammoniated aflatoxin contaminated corn in pregnant rabbits

This paper dealt with the effects of ammoniated aflatoxin-contaminated corn under atmospheric pressure [AP], on the biochemical and pathological changes of pregnant rabbits. Two groups of female pregnant rabbits were fed on control diet and ammoniated aflatoxin- contaminated diet throughout pregnancy [trial 1]. Another 2 groups were fed the same diets during the second half of pregnancy [trial 2]. Rabbits fed ammoniated ration in both trials 1 and 2 completed pregnancy accompanied by normal parturition and alive offspring. No mortalities were recorded among treated rabbits. Progesterone hormone was not significantly affected in treated rabbits, while levels of blood metabolites and enzymes showed mild variations. Postmortem examination of sacrificed rabbits revealed mild pathological changes