Pathological and molecular studies on avian leukosis virus subgroup [J] in chicken parents flocks

The present study was carried out on 10 parent chickens flocks collected from different provinces. The examined cases were classified into 47 commercial layers and 198 broiler parents to study the gross and microscopical lesions of different tumors induced by avian leukosis subgroup-J [ALV-J] in broiler breeders and layers flocks. Serological tests were conducted on 245 serum samples to detect the antibodies of ALV-J. Polymerase chain reaction PCR was carried out on tissue samples to confirm the presence of DNA of ALV subgroup J. Myeloid leukosis was recorded in four broiler flocks induced by the novel sub group-J of ALV. Pathological findings of infected parent chickens flocks revealed multiple neoplastic nodules in different organs including liver, kidney, gonads, intestine, skeletal muscle and bone. Histologically, the main constituent of tumor lesions was mature and immature myelocytes. Nephroblastoma was considered as the first recorded cases in Egyptian broiler parent flocks affected with ALV subgroup J. The results of this work concluded that the histopathological examination plays a crucial and decisive role in diagnosing different types of neoplasm caused by avian leukosis virus subgroup-J. The application of PCR test verified the presence of DNA of ALV-J that induced the tumors of myeloid leukosis

Serological and pathological studies on field cases of brucellosis in deer [Dorcas gazelle]

During this study, 26 deer [Dorcas gazelle] were examined for brucellosis. Sera were separated and tested by using the Rose Bengal Plate Test [RBPT] and serum agglutination test [SAT]. Fifteen of examined animals showed high titer of serum antibodies to brucella species and the sera of the examined deer gave doubtful results for brucella infection. Pathological studies were carried out using specimens from the swollen joints and testicles. Histopathological examinations of the joints revealed areas of edema, necrosis and inflammatory cells infiltration. Number of cases showed fibrinoid degeneration, fibrous connective tissue proliferation and granulomatous inflammation. Changes of the tests and epididymis varied from degenerative changes to necrosis and atrophied seminiferous tubules. Also, the presence of the characteristic multinucleated giant cells in the lumen of the degenerated seminiferous tubules were recognized