ABSTRACT
The major sterol of epicoprostanol was isolated from ambergris by flash column chromatography. Its identity as epicoprostanol was confirmed by 1H - NMR and 13C - NMR spectroscopy. Epicoprostanol was studied for its anti - inflammatory and antipyretic activities. Epicoprostanol produced a significant inhibition of carrageenan induced paw oedema. The study on antipyretic activity showed, that epicoprostanol produced a significant reduction of yeast - induced hyperthermia in rat
Subject(s)
Anti-Inflammatory AgentsABSTRACT
Periplogenin was isolated as the major biotransformation product of digitoxigenin by both P. tomentosa and A. visnaga cell suspension cultures. In addition, 1 beta hydroxydigitoxigenin and uzarigenin were also obtained in both cultures. These products were identified by HPLC, MS, 1H- and 13C-NMR analyses. The suspension cultures of both plants, though taxonomically distinct, proved quite closely related biochemically as far as digtioxigenin metabolism is concerned. A. visnaga culture, however,was a more active biotransformation system, metabolizing 65% of digitoxigenin in one week as compared to 16% by beta. Tomentosa cultures. 5 beta -Hydroxylation, 1beta -hydroxylation and 5-epimerization were demonstrated by both suspension cultures. This represents the first report of 1beta -hydroxydigitoxigenin and uzarigenin as digitoxigenin biotransformation products using cell cultures of higher plants
Subject(s)
Tissue Culture Techniques , Biotransformation , Chromatography , Plant ExtractsABSTRACT
Three alkaloids were isolated from the legumes [without seeds] of the Saudi Rhazya stricta Decaisne. The alkaloids were obtained from the chloroform extract [pH6] and were identified as [-]- quebrachamine, [+]- eburenine and [+]-akuammidine by 1H-NMR,13C-NMR and 2D-NMR. The hitherto unreported 13C-NMR data for eburenine has been assigned. This represents the first report for the occurrence of these three alkaloids in the legumes of R. stricta