Effect of dietary energy on the superovulatory response to PMSG treatment, with special reference to embryo quality, metabolic and endocrine response in female goats

Thirty Baladi non lactating, goats were randomly allocated according to their dietary treatments into 3 groups. Group I [Restricted diet], group II [Flushing diet] and group III [High fat diet]. The oestrous cycle were synchronized with progestagen impregnated intravaginal sponges [MPA], for 14 days, followed by injection of PMSG at day 13, and HCG at day 14, for all female goates. At day 5-6 post oestrus, the embryos/ova were recovered surgically and examined for evaluation. The onset and duration of oestrus varied significantly between the groups. It appeared earlier in animals in group II and III than in group I. The total ovarian response, increased in group III and decreased in group II, compared with group I. However, the average number of normal corpora lutea was non significantly affected by feeding regimens. The data also revealed that, the total percentage of transferable embryos [grades 1, 2 and 3] was significantly elevated in groups II and III, compared with group I. Similarly the recovery rate was higher in groups II and III. At day of embryo recovery, the concentrations of serum progesterone have remarkable increase in groups II and III. In addition, the level of estradiol-17beta recorded a peak level at the day of oestrus. This level dropped to record a lowest values at day of embryo recovery. Serum triglycerides, cholesterol and HDL-CH were lowered in feed restricted diet compared with high fat level. Regarding serum insulin concentration, goats received the energy restricted diet had a significant decrease in serum insulin level than those received flushing or high fat diets. It is concluded that the level of dietary energy had a significant effect on the onset and duration of oestrus, ovarian activity and quality of embryos available for transfer, but increased the number of unovulatory follicles

Diagnosis of rickets and kwashiorkor by serum and cerebrospinal fluid electrophoretic separation of protein fractions

Thirty children suffering from Kwashiorkor, 32 children suffering from Rickets and 30 normal healthy children were admitted in Mounira Children Hospital of Cairo University for the determination of total protein as well as electrophoretic fraction of serum and cerebrospinal fluid [CSF] proteins. The results obtained proved the incidence of variable fluctuation in serum and C.S.F. proteins. In case of Kwashiorkor, a decrease in serum albumin was accompanied by an increase in CSF- albumin. The serum gamma -globulin increased, where as the CSF-beta and gamma -globulin decreased. The changes indicated in both serum and CSF-protein fractions [albumin and gamma -globulin] of Rackitic children were identical. The pattern of fluctuation were similar to those indicated in C.S.F. of Kwashiorkor

Serum proteins and triglycerides after oxamniquine administration in normal and schistosomal infected mice

The effect of oxamniquine on serum proteins and triglycerides was studied in mice. A single oral dose of oxamniquine [50 mg/kg b.w.] was administered after three months from the schistosomal infection. The same dose was given also to normal control group of animals. Blood samples were withdrawn prior to drug administration and periodically every two weeks for two months. It was concluded that oxamniquine caused an elevation on serum alpha1, alpha2 beta and total globulins in normal mice. In schistosomal infected mice, oxamniquine administration inhibited the abnormalities in A/G ratio and significantly decreased triglycerides