ABSTRACT
The reaction of secondary amines with carbon disulphide to form dithiocarbamic acid was utilized as a base for analysis of propranolol hydrochloride. The dithiocarbamic acid formed reacts with Cu [II] to form a yellow colored complex measured at 440 nm after extraction with benzene. Conditions of the reaction were thoroughly studied including the effect of acetic acid and pyridine concentrations on the process of extraction of dithiocarbamate complex. The results obtained by this method were compared with the USP method. The results revealed that both the variance ratio and t test are within the theoretical limits, indicating that the suggested method is equally precise and accurate. Beer's law obeyed for the concentration range between 50 and 400 ug.m-1 with mean percentage recovery of 100.16 + 0.60
Subject(s)
ColorimetryABSTRACT
A method is presented for the determination of chloramphenicol and thiamphenicol in the presence of their degradation products using first derivative spectrophotometry by measuring the absolute value of the first derivative curves at the zero contribution of the corresponding degradation products, the concentration of the intact drug can be calculated directly without interference of the degradation product. The method was proved using laboratory prepared mixtures of the intact drugs with their degradation products
Subject(s)
Thiamphenicol , SpectrophotometryABSTRACT
A method is presented for the direct determination of amoxacillin in the presence of dicloxacillin or flucloxacillin without prior separation using first and second derivative spectra [AD 1 and AD 2] at the zero contribution of flucloxacillin or dicloxacillin. The method was proved using laboratory prepared mixtures of amoxacillin with flucloxacillin or dicloxacillin