Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add filters








Year range
1.
Japanese Journal of Physical Fitness and Sports Medicine ; : 138-144, 1993.
Article in Japanese | WPRIM | ID: wpr-371608

ABSTRACT

A study was conducted to investigate the changes in human urinary proteins caused by physical exercise. Nine subjects (male middle-distance runners, 19-21 years) were loaded physical exercise with a 10km-run, and quantitative and qualitative changes in urinary proteins were examined using various techniques including polyacrylamide gel electrophoresis, immunoelectrophoresis and two-dimensional electrophoresis. Total protein concentrations in urine from all subjects before exercise were very low (0.68-2.56mg/d<I>l</I>), but those were increased remarkably after exercise. Few proteins except for albumin were detected in urine before run by polyacrylamide gel electrophoresis and immunoelectrophoresis, however, a few proteins were observed after exercise in addition to a marked increase of albumin. Several proteins which were not observed in serum could also be detected in urine after exercise by two-dimensional electrophoresis under non-denaturing condition. It is considered that two-dimensional electrophoresis is a very powerful technique for analyzing dilute and complicated protein mixture in biological fluids such as urine.

SELECTION OF CITATIONS
SEARCH DETAIL