ABSTRACT
Objective: Poly [2-methacryloyloxyethyl phosphoryl choline [MPC]-co-n-buthyl methacrylate [BMA]-co-p-nitrophenyl-oxycrabonyl poly ethylene glycol-methacrylate [MEONP]] [PMBN], a biocompatible terpolymer, is a unique polymer with applications that range from drug delivery systems [DDS] to scaffolds and biomedical devices. In this research, we have prepared a monomer of p-nitrophenyl-oxycarbonyl poly [ethylene glycol] methacrylate [MEONP] to synthesize this polymer. Next, we designed and prepared a smart, water soluble, amphiphilic PMBN polymer composed of MPC, BMA, and MEONP
Materials and Methods: In this experimental study, we dissolved MPC [4 mmol, 40% mole fraction], BMA [5 mmol, 50% mole fraction], and MEONP [1 mmol, 10% mole fraction] in 20 ml of dry ethanol in two necked flasks equipped with inlet-outlet gas. The structural characteristics of the synthesized monomer and polymer were determined by Fourier transform infrared spectroscopy [FT-IR], proton nuclear magnetic resonance [H-NMR], dynamic light scattering [DLS], gel permeation chromatography [GPC], scanning electron microscope [SEM], and transmission electron microscope [TEM] analyses for the first time. We treated the polymer with two different cell lines to determine its biocompatibility
Results: FT-IR and H-NMR analyses confirmed the synthesis of the polymer. The size of polymer was approximately 40 nm with a molecular weight [MW] of 52 kDa, which would be excellent for a nano carrier. Microscopic analyses showed that the polymer was rod-shaped. This polymer had no toxicity for individual cells
Conclusion: We report here, for the first time, the full properties of the PMBN polymer. The approximately 40 nm size with an acceptable zeta potential range of -8.47, PDI of 0.1, and rod-shaped structure indicated adequate parameters of a nanopolymer for nano bio-applications. We used this polymer to design a new smart nano carrier to treat leukemia stem cells based on a target DDS as a type of bio-application