Effect of deferoxamine of gentamicin-induced acute renal failure in albino rats

The effect of the iron chelator, deferoxamine, on gentamicin- induced acute renal failure was examined in albino rats. Rats treated with gentamicin alone [100 mg/kg body weight/day by subcutaneous route for 8 consecutive days] developed acute renal failure [plasma urea 171.2 +/- 34.5 mg/dl and creatinine 3.11 +/- 0.53 mg/dl] compared with saline treated controls [plasma urea 39 +/- 13.7 and creatinine 0.26 +/- 0.1 ms/dl]. In contrast, rats treated with gentamicin and deferoxamine [100 mg/kg body weight/day by intraperitoneal route in 2 divided doses] had significantly lower plasma urea and creatinine levels [62.2 +/- 16.3 mg/dl and 0.72 +/- 0.21 mg/dl, respectively] compared with gentamicin only-treated rats. In addition, malondialdehyde content in renal cortical tissue was significantly lower in rats treated with gentamicin plus deferoxamine [2.05 +/- 0.12 nmol/mg protein] when compared with gentamicin only-treated rats [3.7 +/- 0.27 nmol/mg protein]

Effect of desferoxamine and urine alkalinization on myoglobin-induced kidney injury in albino rats

Myoglobinuric acute renal failure is a known complication of several clinical sittings involving muscle damage. Renal damage has been partially attributed to iron released from myoglobin, which then catalyzes the generation of reactive oxygen species and oxidant tissue injury. To define whether desferrioxamine [iron chelator] and urine alkalinization would confer direct and additive protective effects on myoglobin-induced renal failure, glycerol model of myoglobinuric acute renal failure in albino rats was used. Rhabdomyolysis was induced by injecting hypertonic glycerol solution in saline [50%], at a dose of 8 ml/kg body weight, in the muscles of the hind limbs of rats. Forty adult male rats were randomized into five groups, eight rats/group. The 1st group was injected with glycerol solution, the 2nd with glycerol and intra-peritoneal desferrioxamine at a dose level of 50 mg/kg body weight twice daily, the 3rd with glycerol and given 0.28 molar sodium bicarbonate drinking solution, the 4th with glycerol and desferrioxamine and was given the alkali drinking solution, and the 5th [control group] with normal saline and desferrioxamine and given the alkali drinking solution. The results showed that desferrioxamine and urine alkalinization reduce oxidative stress in renal tissue and protect against functional and structural myoglobinuric renal damage, and both treatment regimens provide additive protective effects

Captopril promotes the protective effect of deferoxamine in acute parquat intoxicated rats

The present study investigated the effect of captopril and deferoxamine on the lung of paraquat intoxicated rats. Animals were classified into five groups, each composed of 12 rats. The 1st group served as control and received i.p. saline twice daily. The 2nd group injected i.p. with 1 dose of LD100 [35 mg/kg]. The 3rd group received deferoxamine 100 mg/kg in 2 divided doses for 14 days starting at 24 hours before paraquat injection. The 4th group received captopril in drinking water [50 mg/L] for 4 weeks before paraquat injection. The 5th group received captopril in drinking water in non hypotensive dose [50 mg/L] for four weeks before paraquat injection and injected with deferoxamine i.p. at 100 mg/kg/day in 2 divided doses for 14 days, starting at 24 hours before paraquat injection. The survival time and%, lipid peroxidation and lung histology were carried out. All surviving animals were killed at 14th day after administration of paraquat

Reversal of drug-induced hyperalgesia by transient hyperglycaemia

This study was conducted to assess the effect of transient hyperglycemia on drug-induced hyperalgesia. The study included male mice [18-25 g] randomly divided into groups of six to eight animals. Nociceptive sensitivity was estimated by the hot-plate assay [50C]. Naloxone per se exerted hyperalgesic activity at 0.5 and 4 mg/kg, whereas the middle dose [2 mg/kg] produced a slight increase in nociceptive threshold. Yohimbine produced a biphasic response, i.e. it induced significant hyperalgesic effect in the lower dose range and marked elevation in nociceptive threshold in the highest dose. On the other hand, atropine produced consistent and dose dependent hyperalgesic effect. The hyperalgesic responses induced by all three antagonists were uniformly reversed by transient hyperglycemia. The findings regarding naloxone would suggest that it would be valuable in dealing with painful diabetic neuropathy

Studies on neurologic functional and structural changes in acrylamide toxicity [an experimental study]

This work was designed to study the toxic effects of acrylamide and glycidamide in vivo on the isolated Sprague Dawley rats to compare the toxic effects of acrylamide versus its epoxide metabolite, glycidamide on the peripheral nerves. Acrylamide toxicity was induced by a daily i.p. injection of 25 and 50 mg/kg body weight/day, respectively, for 8 days. The weight of the rats, performances of the rotarod test and hind limb splay was performed every other day. On the 9th day, the animals were sacrificed and sciatic nerves were removed and examined. The results are presented and described