Why are children different in their moderate-to-vigorous physical activity levels? A multilevel analysis / Por que as crianças são diferentes em seus níveis de atividade física de intensidade moderada a vigorosa? Uma análise multinível

Abstract Objective: Children's differences in moderate-to-vigorous physical activity levels are not at random. This study investigates the relevance of individual- and school-level characteristics in explaining these differences. Methods: In total, 307 children (154 girls) aged 5-10 years, from 19 Portuguese schools, were sampled. Height and weight were measured, and body mass index was calculated. Time spent in moderate-to-vigorous physical activity was measured by accelerometry. Gross motor coordination was assessed with the KörperkoordinationsTest für Kinder battery and socio-economic status was obtained via the school social support system. School characteristics were obtained with an objective school audit. A multilevel analysis was used as implemented in Stata 15. Results: Schools explained 18.2% of the total variance in moderate-to-vigorous physical activity, with the remainder being ascribed to children's distinct characteristics. Boys were more active (β = 29.59 ± 11.52, p < 0.05), and having higher gross motor coordination levels (β = 0.11 ± 0.04, p < 0.05) was positively associated with daily moderate-to-vigorous physical activity, whereas being older (β = −5.00 ± 1.57, p < 0.05) and having higher socio-economic status (β = −7.89 ± 3.12, p < 0.05) were negatively related with moderate-to-vigorous physical activity. From the school-level correlates, only playground dimension was significantly associated with moderate-to-vigorous physical activity levels. Children from schools with medium (40 m2 to 69 m2) and large playground dimensions (≥70 m2) were less active than children with smaller playground dimensions (10 m2 to 39 m2). Conclusions: Variation in school children's moderate-to-vigorous physical activity is mostly explained by their individual characteristics; school characteristics also play a role but to a smaller degree. Future intervention programs to change this behavior should be more personalized, emphasizing mostly individual-level characteristics.

Resumo Objetivo As diferenças entre crianças na atividade física moderada a vigorosa não são aleatórias. Este estudo investiga a relevância das características em níveis individuais e escolares para explicar essas diferenças. Métodos Foram amostradas 307 crianças (154 meninas) entre 5 e 10 anos, de 19 escolas portuguesas. A estatura e o peso foram medidos e o índice de massa corporal foi calculado. O tempo gasto em atividade física moderada a vigorosa foi medido por acelerometria. A coordenação motora grossa foi avaliada com a bateria do Körperkoordinationstest für Kinder e o status socioeconômico foi obtido através do sistema de apoio social da escola. As características da escola foram obtidas através de uma auditoria escolar objetiva. Uma análise multinível foi utilizada como implantada no Stata 15. Resultados As escolas explicaram 18,2% da variância total da atividade física moderada a vigorosa, com o restante atribuído às diferentes características das crianças. Os meninos foram mais ativos (β = 29,59 ± 11,52, p < 0,05) e o fato de ter níveis mais altos de coordenação motora grossa (β = 0,11 ± 0,04, p < 0,05) foi positivamente associado com a atividade física moderada a vigorosa diária. Os mais velhos (β = -5,00 ± 1,57, p < 0,05) e com maior status socioeconômico (β = -7,89 ± 3,12, p < 0,05) foram negativamente relacionados com a atividade física moderada a vigorosa. Com base nos correlatos a nível escolar, apenas a dimensão da área recreativa foi significativamente associada aos níveis de atividade física moderada a vigorosa. As crianças das escolas com área recreativa média (40 m2 a 69 m2) e grande (≥ 70 m2) foram menos ativas do que as crianças com áreas recreativas com menores dimensões (10 m2 a 39 m2). Conclusões A variação na atividade física moderada a vigorosa de escolares é explicada principalmente por suas características individuais; as características da escola também desempenham um papel, mas em menor grau. Futuros programas de intervenção para mudar esse comportamento devem ser mais personalizados, enfatizar principalmente as características em nível individual.

Calcium phosphate carrying simvastatin enhances bone regeneration: a systematic review

Abstract Several studies have aimed to develop alternative therapeutic biomaterials for bone repair. The purpose of this systematic review was to evaluate how statins carried by calcium phosphate affect the formation and regeneration of bone tissue in animal models when compared to other biomaterials or spontaneous healing. This systematic review followed the recommendations of the Cochrane Handbook for Systematic Reviews of Interventions, the PRISMA guidelines, and the Preclinical Systematic Review & Meta-analysis Facility (SyRF). The protocol of this systematic review was registered in PROSPERO (CRD42018091112) and in CAMARADES. In addition, ARRIVE checklists were followed in order to increase the quality and transparency of the search. An electronic search was performed using the MEDLINE/PubMed, Scopus, SciELO, and PROSPERO library databases. The authors used a specific search strategy for each database, and they also conducted a search in the grey literature and cross-references. The eligibility criteria were animal studies, which evaluated bone repair treated with calcium phosphate as a simvastatin carrier. The selection process yielded 8 studies from the 657 retrieved. All manuscripts concluded that locally applied simvastatin carried by calcium phosphate is biocompatible, enhanced bone repair and induced statistically greater bone formation than cloth or calcium phosphate alone. In conclusion, the pertinent pre-clinical studies evidenced the calcium phosphate biocompatibility and its effectiveness in delivering SIM to improve the repair of bone defects. So, clinical trials are encouraged to investigate the impact of SIM associated with calcium phosphate bone graft in repairing bone defect in humans.

Resumo Muitos estudos objetivaram desenvolver biomateriais terapêuticos alternativos para o reparo ósseo. O objetivo desta revisão sistemática foi avaliar o efeito da estatina carreada por fosfato de cálcio na formação e regeneração de tecido ósseo em modelos animais quando comparado com outros biomateriais ou coágulo. Esta revisão sistemática seguiu as recomendações do Cochrane Handbook for Systematic Reviews of Interventions, PRISMA guidelines, e o Preclinical Systematic Review & Meta-analysis Facility (SyRF). O protocolo desta revisão sistemática foi registrado no PROSPERO (CRD42018091112) e no CAMARADES. Além disso, o guia ARRIVE foi utilizado com o objetivo de aumentar a qualidade e transparência do estudo. Uma pesquisa eletrônica foi realizada através do MEDLINE/PubMed, Scopus, SciELO, e biblioteca do PROSPERO. Os autores utilizaram uma estratégia de busca específica para cada base de dados, e uma busca foi conduzida na literatura cinza e nas referências dos artigos selecionados. Os critérios de elegibilidade foram estudos em animais, os quais avaliaram o repara do ósseo tratado com fosfato de cálcio como carreador de estatina. O processo de seleção obteve 8 estudos dos 657 encontrados. Todos os estudos concluíram que a aplicação local da sinvastatina carreada pelo fosfato de cálcio é biocompatível, melhora o reparo ósseo e induz uma formação óssea significantemente maior que coágulo ou fosfato de cálcio sozinho. Em conclusão, os estudos pré-clínicos pertinentes evidenciaram a biocompatibilidade do fosfato de cálcio e sua eficácia na entrega do SIM para melhorar o reparo de defeitos ósseos. Assim, estudos clínicos são encorajados a investigar o impacto do SIM associado ao enxerto ósseo de fosfato de cálcio na reparação de defeito ósseo em humanos.

Triatomine bugs (Hemiptera, Reduviidae, Triatominae) in the Domiciles of the Guaribas Valley Territory, in Northeastern Brazil

Abstract INTRODUCTION Triatomine bugs are hematophagous insects that are extremely important in public health because they are natural vectors of Trypanosoma cruzi, the causative agent of Chagas disease. In this study, we aimed to assess the occurrence of triatomine species and the natural T. cruzi infection in the Guaribas Valley territory, an endemic region for Chagas disease in northeastern Brazil. METHODS Insects were actively captured from July 2017 to October 2019 in the intra- and peridomiciles of 16 municipalities of the Guaribas Valley territory, in the southeast area of Piauí state. Triatomine species were identified following a taxonomic key, and natural infection was investigated through insects' fresh feces exams. RESULTS A total of 430 triatomines were collected, including 211 nymphs and 219 adults. Of all collected specimens, 39 (9.1%) were from the intradomiciles and 391 (90.9%) from peridomiciles. Nine species, including two subspecies, could be identified: Triatoma brasiliensis brasiliensis, T. brasiliensis macromelasoma, T. pseudomaculata, T. sordida, T. juazeirensis, T. melanocephala, Panstrongylus lutzi, Rhodnius domesticus, R. nasutus, and R. robustus. T. brasiliensis were the most frequently collected bugs, representing 72% of all the identified insects. None of the examined invertebrates presented flagellate forms of T. cruzi. CONCLUSIONS: This is the first report of T. b. macromelasoma and T. juazeirensis in the Guaribas Valley territory. The persistence of triatomine species in the domiciles in an endemic area for Chagas disease emphasizes the relevance of entomological surveillance and vector control measures in the studied area.

Familial aggregation and heritability of markers of metabolic risk, physical activity, and physical fitness in nuclear families from Muzambinho (Minas Gerais, Brazil)

ABSTRACT Objective This study investigated the familial aggregation and heritability of markers of metabolic risk, physical activity, and physical fitness in nuclear families from Muzambinho (Minas Gerais, Brazil). Subjects and methods The study included members of 139 families, comprising 97 fathers (aged 40 ± 7 years), 129 mothers (35 ± 6 years), 136 sons (12 ± 4 years), and 121 daughters (12 ± 5 years). Evaluated markers included (A) body mass index, waist circumference, glycemia, and cholesterolemia, as metabolic risk markers; (B) total weekly volume of physical activity, as a physical activity marker; and (C) relative muscle strength, as a physical fitness marker. Correlations between family members and heritability (h2) were estimated using the software S.A.G.E. Results Significant familial correlations were obtained between parents-offspring for glycemia and cholesterolemia (both ρ = 0.21, p < 0.05) and relative muscle strength (ρ = 0.23, p < 0.05), and between siblings for waist circumference, glycemia, total weekly volume of physical activity, and relative muscle strength (ρ variation 0.25 to 0.36, p < 0.05). Heritability values were significant for almost all variables (h2 variations: 20% to 57% for metabolic risk markers, 22% for the total weekly volume of physical activity, and 50% for relative muscle strength), except for waist circumference (h2 = 15%, p = 0.059). Conclusion The presence of significant correlations between family members and/or significant heritability strengthens the possible genetic and/or common familial environment influence on metabolic risk markers, total weekly volume of physical activity, and relative muscle strength.

Seleção em ginástica artística feminina no Brasil / Women artistic gymnastics selection in Brazil / Selección de gimnasia artística femenina de Brasil

Resumo O estudo aborda procedimentos adotados por 40 treinadores na detecção e seleção de ginastas e caracteriza clubes brasileiros que têm programas de seleção (PS). Em 70% dos casos o treinador é responsável pela tomada de decisão na detecção; 90% usam testes motores (técnico-específicos, velocidade, força e flexibilidade). O método misto é o mais usado (55%), contém dados antropométricos, motores e técnicos (70,0%, 62,5%, 47,5%, respectivamente). A maioria dos clubes (61,5%) faz recrutamento periódico; 38,5% usam a forma externa de divulgação. Apenas oito clubes (30,8%) têm PS e esses têm 30% das ginastas de elite.

Abstract This study investigates coaches' (n = 40) procedures in gymnastics selection, and characterizes detection and selection programs (PS) implemented in Brazilian clubs. In 70% of the cases, the coach is the main decision maker; 90% uses motor tests (specific motor skills, speed, strength and flexibility) in PS. The mixed method is the most often used (55%) and comprises anthropometric, motor and technical data (70.0%, 62.5%, 47.5%, respectively). Most clubs (61.5%) conduct periodic recruitment; 38.5% use an external form. Only 8 clubs (30.8%) have PS, and these have 30% of elite gymnasts.

Resumen El presente estudio aborda los procedimientos empleados por 40 entrenadores de la selección de gimnasia y describe los clubes brasileños que cuentan con programas de selección (PS). En el 70% de los casos, el entrenador es el responsable de la toma de decisiones en la detección de las gimnastas; el 90% utiliza pruebas motoras (técnico-específicas, velocidad, fuerza y flexibilidad). El método mixto es el más utilizado (55%) e incluye datos antropométricos, motores y técnicos (70,0; 62,5, y 47,5%, respectivamente). La mayoría de los clubes (61,5%) realizan reclutamientos de manera periódica; el 38,5% de ellos lo hace de manera externa. Solo 8 clubes (30,8%) tienen PS y estos cuentan con el 30% de las gimnastas de élite.

Determination of tnf-a gene polymorphisms on skeletal pattern in class ii malocclusion

Abstract Bone development and growth is a non-going, life-long process, varying greatly among individuals and much of this variation could be modulated by genetic factors. The purpose of this study was to evaluate the association between the polymorphisms in the TNF-a gene and skeletal class II malocclusion. Single nucleotide polymorphisms in TNF-a (rs1799724; rs1800629) gene were studied in 79 skeletal class II malocclusion and 102 skeletal class I malocclusion subjects from Straight Wire Group of Studies on Orthodontics and Functional Orthopedics for Maxillary from Rio de Janeiro, Brazil. The Genotyping of these selected polymorphisms was carried out by TaqMan real-time PCR using genomic DNA extracted from buccal cells. All allele and genotype frequencies were compared between the groups using the PLINK® software in a free, in a dominant and in a recessive model using a chi-square test (p≤0.05). There was no significant association of TNF-a (rs1799724; rs1800629) genotype and allele distribution with skeletal class II malocclusion. Regardless of the dominant or recessive genetic model, the preferential genotype associations for rs1799724 and rs1800629 was insignificant. In conclusion, no evidence of association is apparent between genetic polymorphisms involving TNF-a and skeletal class II malocclusion or the position of the maxilla and mandible in the postero-anterior direction.

Resumo O desenvolvimento e crescimento ósseo é um processo contínuo, que dura toda a vida, variando muito entre os indivíduos e grande parte dessa variação pode ser modulada por fatores genéticos. O objetivo deste estudo foi avaliar a associação entre os polimorfismos no gene TNF-a e a má oclusão da classe II esquelética. Polimorfismos no gene TNF-a (rs1799724; rs1800629) foram estudados em 79 indivíduos com má oclusão esquelética de classe II e 102 indivíduos com má oclusão esquelética classe I do Grupo de Estudos em Ortodontia e Ortopedia Funcional dos Maxilares do Rio de Janeiro, Brasil. A genotipagem destes polimorfismos foi realizada por PCR em tempo real, através de DNA genômico extraído de células bucais. Todas as frequências alélicas e genotípicas foram comparadas entre os grupos utilizando o software PLINK® em um modelo livre, dominante e recessivo. Foi aplicado o teste do qui-quadrado (p≤0,05). Não houve associação significativa na distribuição genotipica e alélica do gene TNF-a (rs1799724; rs1800629) com a má oclusão de classe II esquelética. Independentemente do modelo genético dominante ou recessivo, as associações genotípicas preferenciais para rs1799724 e rs1800629 foram insignificantes. Pode-se concluir que, não existe evidência de associação entre polimorfismos genéticos envolvendo TNF-a e má oclusão esquelética de classe II ou a posição da maxila e mandíbula na direção póstero-anterior.

Large desmoid tumors in familial adenomatous polyposis: a successful outcome

Desmoid tumors develop from connective tissue, fasciae, and aponeuroses, and may occur in the context of familial adenomatous polyposis or may arise sporadically; also, they may be extra-abdominal, intra-abdominal, or located in the abdominal wall. These benign tumors have a great aggressiveness with a high rate of local recurrence. Familial adenomatous polyposis is an inherited condition with autosomal dominant transmission, and is characterized by the development of multiple colonic and rectal adenomatous polyps, as well as desmoid tumors. We present the case of a 54-year-old woman with germline APC gene mutation, who underwent a total colectomy, subsequently developing two large infiltrative solid intra-abdominal lesions consistent with desmoid tumors. Medical treatment with Cox-2 inhibitors was initiated without result. She was submitted to resection for intestinal obstruction, but developed local recurrence. The lesions were also unresponsive to tamoxifen, and chemotherapy was initiated with dacarbazine plus doxorubicin, switching to vinorelbine plus methotrexate, achieving a good response in all lesions after 12 months. The approach to these intra-abdominal lesions should be progressive, beginning with observation, then a medical approach with non-steroidal anti-inflammatory drugs or with an anti-hormonal agent. Afterwards, if progression is still evident, chemotherapy should be started. Surgery should be reserved for resistance to medical treatment, in palliative situations, or for extra-abdominal or abdominal wall desmoids tumors.

The Management of the Impacted Third Molar: When We Can Choose the Conventional Extraction Instead of the Coronectomy?

The technical difficulties in oral surgery are leading many surgeons to believe that the coronectomy technique is always the best choice for the treatment of impacted third molars in intimate relation with the mandibular nerve. The objective of this study is to discuss conventional extraction and coronectomy, considering its respective advantages and the experience of the surgical team that can shed light on experts allowing to a better reflection on this trend and thus contributes to the best surgical choice. This clinical case reports a patient who presented the inferior third molar with impaction and root apices near of the mandibular nerve. Despite evidence to perform coronectomy, conventional extraction was done. The patient had no complications, displaying mild paresthesia during seven postoperative days. The immediate or late unpredictability of the coronectomy leads us to conclude that conventional extraction should be the technique of choice for the extraction of the third molar in close relation with the mandibular nerve, except in particular cases.

Association between body mass index and individual characteristics and the school context: a multilevel study with Portuguese children / Relação entre o índice de massa corporal e as características individuais e do contexto escolar: um estudo multinível com crianças portuguesas

Abstract Objective The aim of this study was to examine the association between individual and school context characteristics with the body mass index of Portuguese children. Methods The sample comprised 1641 children (847 boys) aged 6-10 years from the North and Central regions of Portugal. Regarding the individual characteristics, age, gender, city of residence, levels of physical activity, and physical fitness were assessed. Concerning the school context characteristics, the surrounding environment, school size, presence of recreational characteristics and space, and presence of a sports court and of physical education classes were considered. Children's body mass index was the dependent variable. The multilevel analysis was carried out in HLM 7.0 software. Results The predictors of the child and the school context explained, respectively, 97.3% and 2.7% of the total body mass index variance. Regarding the individual characteristics, older children, boys, and those who had lower performance at the 1-mile run/walk, curl-up, push-up, and higher performance in trunk lift tests showed higher BMI. Further, urban schools with higher recreational spaces were positively associated with children's body mass index. Conclusion School context variables have a reduced effect on body mass index variation compared to the children's biological and behavioral characteristics. The authors therefore encourage strategies that aim to increasing children's physical fitness levels to help prevent excess weight.

Resumo Objetivo Examinar a associação de características individuais e do contexto escolar no índice de massa corporal de crianças portuguesas. Método A amostra compreendeu 1.641 crianças (847 meninos) de 6 a 10 anos. Em relação às características individuais foram utilizadas informações relativas ao sexo, à idade, à residência, à atividade física e à aptidão física. Em termos de contexto escolar, foram considerados o meio ambiente, o tamanho da escola, a presença de recreio, as características e as dimensões do espaço disponível para o recreio, a existência de quadra poliesportiva e de aulas de educação física. O índice de massa corporal [kg/(m2)] foi a variável dependente. A análise multinível foi efetuada no software HLM 7.0. Resultados Os preditores da criança e do contexto escolar explicaram, respectivamente, 97,3% e 2,7% da variância total do índice de massa corporal. Quanto às características individuais, a idade (mais velhos) e o sexo (meninos), bem como o desempenho reduzido nas provas de corrida/marcha da milha, do curl-up, do push-up e valores elevados no trunk lift, estiveram associados ao aumento no índice de massa corporal. Escolas do meio urbano e escolas com maiores espaços para o recreio também estiveram positivamente associadas ao aumento do índice de massa corporal. Conclusões As variáveis do contexto escolar têm um efeito reduzido na variação do índice de massa corporal comparativamente às características biológicas e comportamentais das crianças. Sugere-se a aplicação de programas que visem ao incremento dos níveis de aptidão física das crianças para prevenir o excesso de peso na infância.

Birth weight, physical growth and body composition in children: A longitudinal study / Peso ao nascer, crescimento físico e composição corporal de crianças: um estudo longitudinal

ABSTRACT Objective To describe children's physical growth (body mass and height) velocity and body composition (fat percentage and Fat Free Mass); to investigate the magnitude of interindividual differences according to age, gender and birth weight categories, as well as to examine the differences in the average trajectories of children with Low Birth Weight and Normal Weight according to international references. Methods The sample consisted of 534 children (279 boys and 255 girls, 7 to 10 years old) evaluated in the first year of study and followed for 3 years with overlap between the ages of 7 and 9 years. Physical growth and body composition measurements included: height, body mass, fat percentage (%Fat) and Fat Free Mass. Multilevel Modelling was used. Results Birth weight was not associated with physical growth and body composition markers at 7 years old or with the velocity of their changes (p>0.05). There were significant interindividual differences in the trajectories of physical growth (height and body mass; p<0.001) and body composition (%Fat and Fat Free Mass; p<0.001). In plotting on international percentile charts, the trajectories of growth and body composition were within expected values for age and gender, regardless of birth weight. Conclusion There are significant differences in the dynamics of stature growth, body mass and Fat Free Mass, and Low Birth Weight has no influence on this trajectory. In addition, values are within the expected range for age and sex.

RESUMO Objetivo Este artigo teve por objetivo descrever a velocidade do crescimento físico (massa corporal e estatura) e a composição corporal (percentual de gordura e massa isenta de gordura) de crianças; investigar a magnitude das diferenças interindividuais em função da idade, gênero e categoria do peso ao nascer; e examinar a diferenciação das trajetórias médias das crianças com Baixo Peso ao Nascer e com Peso Adequado ao Nascer, relativamente às referências internacionais. Métodos A amostra foi composta por 534 crianças (279 meninos e 255 meninas, na faixa etária entre 7 e 10 anos de idade), avaliadas no primeiro ano de estudo e seguidas por três anos, com sobreposição de idade entre 7 e 9 anos. Foram medidas a estatura e a massa corporal, e estimados o percentual de gordura (%Gord) e a Massa Isenta de Gordura. As análises foram realizadas com a Modelagem Multinível. Resultados O peso ao nascer não apresentou associação com as variáveis de crescimento físico e composição corporal aos 7 anos de idade, bem como com seus incrementos dos 7 aos 10 anos (p>0,05). Há diferenças interindividuais significativas nas trajetórias de crescimento físico (estatura, massa corporal; p<0,001) e composição corporal (%Gord e Massa Isenta de Gordura; p<0,001). Na plotagem realizada em cartas percentílicas internacionais, independentemente do peso ao nascer, as trajetórias do crescimento e composição corporal encontram-se dentro dos valores esperados para idade e gênero. Conclusão O estudo concluiu que existem diferenças significativas no dinamismo do crescimento estatural, massa corporal e Massa Isenta de Gordura, e o Baixo Peso ao Nascer não exerce nenhuma influência nessa trajetória. Ademais, os seus valores estão dentro do expectável para idade e gênero.

Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides

Abstract Purpose: To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells. Methods: Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3. Results: Viability of the essential oil-treated cells was significantly higher than the control group at P1 (p = 0.0008). The treatment with the oil, at a concentration of 1 µg/mL, led to increases of 24.8% at P1 and 43.0% at P3 in the rate of cell proliferation compared with control cells. Conclusion: Supplementing culture medium with essential oil of Lippia origanoides increased cell proliferation, especially at later passages.

Relação entre atividade física, aptidão física e risco cardiovascular: estudo em muzambinho, minas gerais / Relationship between physical activity, physical fitness and cardiovascular risk: study in muzambinho, minas gerais / Relación entre actividad física, aptitud física y riesgo cardiovascular: estudio en muzambinho, minas gerais

RESUMO Introdução: A relação negativa entre atividade física, aptidão física e risco cardiovascular está bem estabelecida em populações com baixos níveis de atividade física. Objetivo: O presente estudo investigou essa relação em adultos de Muzambinho, Minas Gerais, uma população com alta taxa de indivíduos fisicamente ativos. Métodos: Participaram 237 indivíduos (132 mulheres), na maioria jovens (44,3% entre 20 e 39 anos). Foram avaliados indicadores de risco cardiovascular (índice de massa corporal, circunferência da cintura, glicemia de jejum, colesterolemia de jejum e pressão arterial sistólica e diastólica), atividade física (volume semanal de atividade física total) e aptidão física (aptidão aeróbica e força manual relativa). Resultados: Mais de 90% dos indivíduos foram classificados como ativos (praticavam atividade física por mais de 150 min/semana). A regressão linear identificou associação negativa da força manual relativa e da aptidão aeróbica ao índice de massa corporal e a circunferência da cintura, bem como associação positiva da força manual relativa à pressão arterial diastólica. Os indicadores de atividade física e aptidão física não se associaram ao risco cardiovascular global. Conclusão: Nessa população, a relação inversa entre atividade física, aptidão física e risco cardiovascular limitou-se aos indicadores de aptidão física e aos de obesidade, sugerindo que em populações com alta taxa de indivíduos fisicamente ativos, o aumento da aptidão física, mas não da atividade física, pode ajudar a reduzir a obesidade. Nível de Evidência II; ECRC de menor qualidade.

ABSTRACT Introduction: The negative relationship between physical activity, physical fitness, and cardiovascular risk is well established in populations with low levels of physical activity. Objective: The present study investigated this relationship in adults from Muzambinho, Minas Gerais, a population with a high rate of physically active individuals. Methods: Two hundred and thirty-seven individuals (132 women), mostly young adults (44.3% between 20 and 39 years) were assessed. Cardiovascular risk predictors (body mass index, waist circumference, fasting blood glucose, fasting cholesterol, and systolic and diastolic blood pressure), physical activity (total weekly volume of total physical activity) and physical fitness (aerobic fitness and relative hand strength) were measured. Results: More than 90% of the individuals were classified as active (they practiced physical activity for more than 150 min/week). Linear regression identified a negative association of relative hand strength and aerobic fitness to body mass index and waist circumference, as well as a positive association of relative hand strength with diastolic blood pressure. The indicators of physical activity and physical fitness showed no association with global cardiovascular risk. Conclusion: In this population, the inverse relationship between physical activity, physical fitness and cardiovascular risk was limited to physical fitness and obesity indicators, suggesting that in populations with high rates of physically active individuals, the increase in physical fitness, but not in physical activity, can help reduce obesity. Level of Evidence II; Lesser quality RCT.

RESUMEN Introducción: La relación negativa entre actividad física, aptitud física y riesgo cardiovascular está bien establecida en poblaciones con bajos niveles de actividad física. Objetivo: Este estudio investigó esta relación en adultos de Muzambinho, Minas Gerais, una población con una alta tasa de individuos físicamente activos. Métodos: Participaron 237 individuos (132 mujeres), en su mayoría jóvenes (44,3% entre los 20 y 39 años). Se evaluaron indicadores de riesgo cardiovascular (índice de masa corporal, circunferencia de la cintura, glucosa en ayunas, colesterol en ayunas y presión arterial sistólica y diastólica), actividad física (volumen semanal de actividad física total) y aptitud física (capacidad aeróbica y fuerza relativa de la mano). Resultados: Más del 90% de los individuos fueron clasificados como activos (practicaban actividad física por más de 150 min/semana). La regresión lineal identificó asociación negativa de la fuerza relativa de la mano y la capacidad aeróbica al índice de masa corporal y la circunferencia de la cintura, así como la asociación positiva de la fuerza relativa de la mano a la presión arterial diastólica. Los indicadores de actividad física y aptitud física no se asociaron al riesgo cardiovascular global. Conclusión: En esta población, la relación inversa entre actividad física, aptitud física y riesgo cardiovascular se limitó a los indicadores de aptitud física y obesidad, lo que sugiere que en poblaciones con alta tasa de individuos físicamente activos, el aumento de la aptitud física, pero no de la actividad física, puede ayudar a reducir la obesidad. Nivel de Evidencia II; ECRC de menor calidad.

Alveolar bone repair with strontium- containing nanostructured carbonated hydroxyapatite

ABSTRACT Objective: This study aimed to evaluate bone repair in rat dental sockets after implanting nanostructured carbonated hydroxyapatite/sodium alginate (CHA) and nanostructured carbonated hydroxyapatite/sodium alginate containing 5% strontium microspheres (SrCHA) as bone substitute materials. Methods: Twenty male Wistar rats were randomly divided into two experimental groups: CHA and SrCHA (n=5/period/group). After one and 6 weeks of extraction of the right maxillary central incisor and biomaterial implantation, 5 μm bone blocks were obtained for histomorphometric evaluation. The parameters evaluated were remaining biomaterial, loose connective tissue and newly formed bone in a standard area. Statistical analysis was performed by Mann-Withney and and Wilcoxon tests at 95% level of significance. Results: The histomorphometric results showed that the microspheres showed similar fragmentation and bio-absorbation (p>0.05). We observed the formation of new bones in both groups during the same experimental periods; however, the new bone formation differed significantly between the weeks 1 and 6 (p=0.0039) in both groups. Conclusion: The CHA and SrCHA biomaterials were biocompatible, osteoconductive and bioabsorbable, indicating their great potential for clinical use as bone substitutes.

Accelerated Healing Period with Hydrophilic Implant Placed in Sheep Tibia

Abstract The objective of this study was to evaluate the early osseointegration of two different implants surfaces, a sandblasted and acid-etched surface (TN) compared with same geometry and surface roughness modified to be hydrophilic/wettable by conditioning in an isotonic solution of 0.9% sodium-chloride (TA) through histological and histomorphometric analysis after sheep tibia implantation. Forty dental implants, divided in two groups (TN and TA) were placed in the left tibia of twenty healthy, skeletally mature Santa Ines sheep (n=5/experimental period). After 7, 14, 21 and 28 days post-implantation, the samples were removed and the sheep were kept alive. Analysis of resin sections (30 μm) allowed the quantification of bone area (BA) and bone-to-implant contact (BIC). TA group presented nearly 50% increase in BA at 14 days (p<0.001, ANOVA - Tukey's post test) compared with 7 days. The TA presented higher values than the TN for BA and BIC at 14, 21, and 28 days after placement, stabilizing bone healing. TA hydrophilic surface promoted early osseointegration at 14 and 21 days compared to TN, accelerating bone healing period post-implant placement in sheep tibia.

Resumo O objetivo deste estudo foi avaliar a osseointegração precoce de duas superficies diferentes de implantes, uma superfície tratada com jateamento e ataque ácido (TN) comparada com outra superfície com a mesma geometria e rugosidade e modificada com hidrofilicidade / molhabilidade através do condicionamento em solução isotônica de 0,9% de cloreto de sódio (TA) através de análises histológica e histomorfométrica após a implantação em tíbia de ovelhas. Quarenta implantes dentários, divididos em dois grupos (TN e TA), foram implantados na tíbia esquerda de vinte ovelhas Santa Inês (n=5/período experimental) saudáveis e esqueleticamente maduras. Após 7, 14, 21 e 28 dias pós-implantação, as amostras foram coletadas e as ovelhas foram mantidas vivas. A análise dos cortes em resina (30 μm) permitiu a quantificação da área de osso (BA) e do contato osso-implante (BIC). O grupo TA apresentou aumento de quase 50% na BA aos 14 dias (p <0,001, ANOVA - pós teste de Tukey) em comparação com 7 dias. O grupo TA apresentou valores maiores do que o TN para BA e BIC aos 14, 21 e 28 dias após a implantação, estabilizando a cicatrização óssea. A superfície hidrofílica TA promoveu uma osseointegração precoce aos 14 e 21 dias em relação à TN, acelerando o período de cicatrização óssea após a instalação dos implantes em tíbias de ovelha.

The game variants in Europe. Trends and perspectives during youth competitive stages

AIMS: The aim of this study was to verify how European countries manage the type of game variants and their frequency during different age groups of youth competition. METHODS: Data were collected from the official rules of youth football championships. To identify countries homogenous groups according to their game variants, Two Step Cluster Analysis procedure was used while a nonparametric Kruskal-Wallis test was used to compare the game variants distribution in each Cluster. In order to correlate the game variants with age groups, a Chi-Square independence test and a Spearman ordinal correlation coefficient were used. The results showed there were five clusters with significant differences in their game variants distribution (X2 kw (4) = 22.149; p<0.001; n = 30) and a significant correlation between age group and game variant (χ2(63) = 477.724; p<0.001; n = 30). Specifically, the most used game variants in each age group were the five-a-side (F5) in Under-8; the nine-a-side (F9) in Under-12; the seven-a-side (F7) in Under-9 and Under10; and the eleven-a-side (F11) in and above Under-13. CONCLUSION: These results may contribute to understand the different country perspectives about the competitive game variants of youth football within the European space and its relation with diverse learning philosophies and pathways.(AU)

A cross-cultural study of physical activity and sedentariness in youth from Mozambique and Portugal

Abstract Aim: The purpose of this study was to analyse the effects of age, sex, body mass index (BMI) and country of origin (Mozambique versus Portugal) on physical activity and sedentariness. Methods: Sample size was comprised of 593 adolescents from Mozambique (n=258) and Portugal (n=335), aged 10-15 years old. Height and weight were measured, and BMI was computed; furthermore, subjects were classified in two groups (normal weight and overweight/obese). The mean time (min/day) spent in moderate-to-vigorous physical activity (MVPA), light physical activity (LPA), and sedentary activity were assessed using the GT3X Actigraph accelerometer over at least 4 days (1 weekend day) with a minimum of 10 hours/day of monitoring. Multiple regression analysis was done in SPSS 20. Results: A low percentage of Mozambican (3.9%) and Portuguese (4.3%) youth complied with the MVPA recommendations (60min·day-1). However, Portuguese boys spend more time in MVPA. Girls and those with higher BMI spend more time in sedentary activities and less time in MVPA and LPA; older adolescents spend more time in sedentary activities and less time in LPA. There is a significant effect of the country of origin on MVPA, favouring Portuguese youth. Conclusions: Results suggest country differences in MVPA between Mozambican and Portuguese youth - the Mozambicans are less involved in MVPA than the Portuguese, probably reinforcing the undesirable effect of the urbanization process in developing nations.

Physico-chemical and Histomorphometric Evaluation of Zinc-containing Hydroxyapatite in Rabbits Calvaria

Abstract The aim of this study was to characterize the physico-chemical properties and bone repair after implantation of zinc-containing nanostructured porous hydroxyapatite scaffold (nZnHA) in rabbits' calvaria. nZnHA powder containing 2% wt/wt zinc and stoichiometric nanostructured porous hydroxyapatite (nHA - control group) were shaped into disc (8 mm) and calcined at 550 °C. Two surgical defects were created in the calvaria of six rabbits (nZnHA and nHA). After 12 weeks, the animals were euthanized and the grafted area was removed, fixed in 10% formalin with 0.1 M phosphate buffered saline and embedded in paraffin (n=10) for histomorphometric evaluation. In addition, one sample from each group (n=2) was embedded in methylmethacrylate for the SEM and EDS analyses. The thermal treatment transformed the nZnHA disc into a biphasic implant composed of Zn-containing HA and Zn-containing β-tricalcium phosphate (ZnHA/βZnTCP). The XRD patterns for the nHA disc were highly crystalline compared to the ZnHA disc. Histological analysis revealed that both materials were biologically compatible and promoted osteoconduction. X-ray fluorescence and MEV-EDS of nZnHA confirmed zinc in the samples. Histomorphometric evaluation revealed the presence of new bone formation in both frameworks but without statistically significant differences (p>0.05), based on the Wilcoxon test. The current study confirmed that both biomaterials improve bone repair, are biocompatible and osteoconductive, and that zinc (2wt%) did not increase the bone repair. Additional in vivo studies are required to investigate the effect of doping hydroxyapatite with a higher Zn concentration.

Resumo O objetivo deste estudo foi caracterizar físico-químicamente e avaliar o reparo ósseo de discos de hidroxiapatita porosa contendo zinco após a implantação em calvária de coelhos (nZnHA). O pó de nZnHA e o pó hidroxiapatita porosa estequiométrica nanoestruturada (controle - nHA) foram confeccionados em discos (8 mm) e calcinados a 550˚C. Dois defeitos cirúrgicos foram criados na calvária de seis coelhos para a implantação dos discos. Após 12 semanas, os animais foram eutanasiados e as áreas enxertadas foram removidas, fixadas em formol a 10% e embebidas em parafina (n=10) para avaliação histomorfométrica. Além disso, uma amostra de cada grupo (n=2) foi embebida em metilmetacrilato para análise de MEV e EDS. O tratamento térmico dos discos de nZnHA transformou-os em implantes bifásicos compostos por HA contendo Zinco e β fosfato tricálcico com Zinco (ZnHA/βZnTCP). Os discos de nHA, apresentaram-se altamente cristalinos e com baixa solubilidade quando comparados aos discos de ZnHA. A análise histológica revelou que ambos os materiais foram biologicamente compatíveis e promoveram a osteocondução. As análises de FRX e MEV-EDS confirmaram a presença do zinco nas amostras de nZnHA. A avaliação histomorfométrica revelou a presença de neoformação óssea em ambos os grupos, porém sem diferenças estatísticas entre eles, com base no teste de Wilcoxon (p>0,05). O presente estudo confirmou que ambos os biomateriais otimizaram o reparo ósseo, foram biocompatíveis e osseocondutivos e a presença do zinco não favoreceu o reparo ósseo. Estudos adicionais in vivo devem ser conduzidos a fim de investigar o efeito de maiores concentrações de zinco.

Physical activity, BMI and metabolic risk in Portuguese adolescents / Atividade física, IMC e risco metabólico em adolescentes portugueses

Abstract It has been reported, in the last decades, a significant decrease in physical activity (PA) levels, with a consequent increase in obesity and metabolic risk factors among youth. The aims of this study were to describe PA levels, the prevalence of overweight/ obesity and metabolic risk factors, and to examine the association between PA and body mass index (BMI) with metabolic risk among Portuguese youth. The sample comprises 212 Portuguese adolescents (12-16 years old). Height and weight were measured. PA was estimated with the Bouchard questionnaire (3 days recall), as well as with the use of a pedometer (used for 5 consecutive days). Metabolic risk factors comprised fasting glucose, triglycerides, HDL-cholesterol, systolic blood pressure and waist circumference. Subjects were classified as normal weight, overweight or obese according to BMI; the maturational status was indirectly estimated with the maturity offset procedure. A continuous metabolic risk score was computed (zMR) and PA values were divided into tertiles. Quisquare test, t-test and ANOVA were used in statistical analyses. SPSS 18.0 and WinPepi softwares were used and p<0.05. A moderate to high prevalence of overweight/obesity and HDL-cholesterol was found, as well as a high prevalence of high blood pressure and low to moderate PA levels among Portuguese youth. The relationship between BMI and zMR showed that obese adolescents have higher zMR when compared to normal weight or overweight adolescents. This finding suggests that increased levels of PA and reduction in the prevalence of overweight/obesity may have a positive role against the development of metabolic risk factors.

Resumo Tem-se verificado, nas últimas décadas, uma redução substancial nos níveis de atividade física (AF), com o consequente aumento da prevalência de sobrepeso/obesidade e fatores de risco metabólico entre jovens. O presente estudo teve por objetivos identificar os níveis de AF e as prevalências de sobrepeso/obesidade e risco metabólico, e associar o índice ponderal com os níveis de AF e risco metabólico em jovens portugueses. A amostra foi composta por 212 jovens portugueses (12-16 anos). Estatura e peso foram mensurados. AF foi estimada a partir do recordatório de 3 dias de Bouchard e pelo uso do pedômetro (durante 5 dias). Os indicadores de risco metabólico considerados foram: glicose, triglicerídeos, HDL-colesterol, tensão arterial sistólica e perímetro da cintura. Os sujeitos foram classificados consoante índice ponderal a partir do índice de massa corporal, enquanto que o estatuto maturacional foi estimado a partir do offset maturacional. Score contínuo de risco metabólico (zRM) foi calculado, e a AF foi dividida em tercis. Os testes estatísticos utilizados foram o Qui-quadrado, teste t independente e ANOVA, as análises foram conduzidas no SPSS 18.0 e WinPepi (p<0,05). Observou-se uma prevalência moderada a elevada de sobrepeso/obesidade e HDL-colesterol, uma elevada prevalência de risco para tensão arterial, e níveis de AF baixos a moderados entre jovens portugueses. A relação entre índice ponderal e zRM mostrou que adolescentes obesos apresentam maior zRM comparativamente aos adolescentes normoponderais ou com sobrepeso. Incrementos nos níveis de AF e redução da prevalência de sobrepeso/obesidade podem ter um papel relevante na redução de fatores de risco metabólico.

Antifungal Action of the Dillapiole-rich Oil of Piper aduncum against Dermatomycoses Caused by Filamentous Fungi.

Aims and Study Design: Piper aduncum L. is a Brazilian plant with many biological properties attributed to its dillapiole-rich essential oil. Despite the development of antibiotics, bacterial and fungal infections are still a public health issue in the medical field. This study measured the antimicrobial activity of the dillapiole-rich essential oil of P. aduncum against pathogenic skin microorganisms. Place of Study: Faculty of Pharmacy and Graduate Program in Pharmaceutical Science, Federal University of Pará, Brazil. This work was performed in 2014. Methodology: Gas Chromatography (GC) and Gas Cromatography-Mass spectrometry (GC-MS) have analyzed the oil and its dillapiole-rich fraction. The determination of Minimum Inhibitory Concentration (MIC) and Minimum fungicidal concentration (MFC) values was carried out by microdilution method and counting of formed colonies. Results: For the strains of Trichophyton mentagrophytes (ATCC 9533 and clinical isolate), the oil and its dillapiole-rich fraction exhibited MIC values of 500 μg/ml while the MFC values were 1,500 μg/ml for the oil and 1,000 μg/ml for the fraction rich in dillapiole. For clinical isolates of T. rubrum and Epidermophyton floccosum, MIC values of 500 μg/ml and MFC 1,500 μg/ml were equal for the oil and the dillapiole-rich fraction, respectively. For clinical isolates of Microsporum canis and M. gypseum, the MIC and MFC values were 250 μg/ml and 500 μg/ml, respectively. For strains of Aspergillus fumigatus (ATCC 40152 and clinical isolate), the oil and its dillapiole-rich fraction have shown the same MIC value of 3.9 μg/ml while the MFC values were 7.8 μg/ml for the strain ATCC 40152, and 15.6 μg/ml for the clinical isolate. The oil and dillapiole-rich fraction did not show antibacterial activity against the strain of Staphylococcus aureus ATCC 6538 and its clinical isolate Conclusion: The dillapiole-rich essential oil of P. aduncum and its dillapiole-rich fraction demonstrates significant antifungal activity against dermatophytes, filamentous fungi and potent antifungal activity against non-dermatophyte filamentous fungi.

Increased overjet is a risk factor for dental trauma in preschool children.

Context: Traumatic dental injury and malocclusion constitute a public health problem due to their high prevalence. Preventing or detecting such conditions, in any population, is of paramount importance. Aim: Assessing the association of anterior occlusal characteristics and dental trauma in preschool children. Settings and Design: A cross‑sectional study was carried out with 2–6‑year‑old randomly selected by a sample calculation, resulting in 606 subjects. Materials and Methods: A questionnaire to collect information about the sample (age, gender, and race) was sent to the children’s parents. Two trained and calibrated examiners (Kappa 0.80) evaluated dental trauma according to criteria established by the World Health Organization. The following anterior occlusal characteristics were evaluated: Normal occlusion, anterior open bite, anterior crossbite, increased overjet (categorized as ≥3 mm), and increased overbite (categorized as ≥3 mm). Statistical Analysis Used: The variables associations were assessed (odds ratio, Chi‑square test, and logistic regression, P < 0.05) using statistical software (SPSS, version 16.0). Results: Dental trauma was observed in 20.8% and malocclusion in 48.6% of the children. There is an association between malocclusion and dental trauma (P = 0.01). Children with malocclusion have a 64% higher chance of suffering dental trauma. Increased overjet was the type of malocclusion related to a higher rate of tooth fracture (P < 0.01). Subjects with this type of malocclusion suffered tooth fractures three times more often than subjects with other malocclusion types. Conclusions: There was association of dental trauma and malocclusion. Increased overjet was the most common malocclusion related to dental trauma. Preventive strategies are needed to reduce the rate of anterior malocclusion and, consequently, dental trauma in preschool children.