ABSTRACT
Urinary erythropoietin from the anemic patients of Chiang Mai Hospital, Chiang Mai University, Chiang Mai, Thailand was prepared and partially purified using various types of colum chromatographic techniques; DEAE-Cellulose, Hydroxyapatite, Sephadex G-25 and Sephadex G-100 Columns. From this investigation, it was found that the actual yield obtained from 30 litres of original urine volume was 65 milligrams, the erythropoietic activity was 686.80 Cobalt Units per milligram and the purification factor was 6,733 times comparing with the Crude Urinary Powder.Key Words : Erythropoietin, anemic patients, column chromatography, erythropoietic activity, purification factor.
ABSTRACT
Twenty rabbits and five sheep were subjected to phenylhydrazine hydrochloride by interaperitoneal injections with dose of 3.0 mg per kilogram of body weight. The sterile solukltion of 2.5 per cent (w v) phenlhydrazine hydrochloride was administered every two days for rabbits, and daily injection for sheep. It was observed that the hemoglobin concentration and hematocrit level caused by phonylhydrazine were approximately 63.2 and 66.5 per cent reduction after administrations for 18 days and 28 days in rabbits and sheep respectively. The erthropoietic activity in anemic palsma filtrate of the experimental animals was also assayed by radioisotope iron incorporatoion measurement. The hormonal activity of rabbits plasma was 0.19 Cobalt Unit per mg of protein while that of sheep’s plasma was 0.17 Cobalt Unit per mg of protein.
ABSTRACT
The\ 24 \– hour\ urinary\ excretion\ of\ erythropoietin\ was\ biologically\ assayed\ in\ the\ 33\ anemic\ patients\ and\ the\ 8\ patients\ with\ protein \– energy\ malnutrition\ from\ Chiang\ Mai\ Hospital,\ Chiang\ Mai\ university,\ Thailand. The\ erythropoietic\ activity\ in\ the\ patients\’\ urine\ was\ measured\ by\ radiobiological\ technique\ in\ starved\ rats,\ comparing\ with\ that\ of\ the\ 18 healthy\ subjects\ living\ in\ the\ same\ area.\ This\ investigation\ indicates\ that\ \ the\ urinary\ erythropoietin\ level\ in\ patients\ with all\ types\ of\ animas\ is\ approximately\ 62\ per\ cent\ higher\ than\ in\ the\ malnourished\ patients.\ The\ average\ urinary\ erythropoietic\ activity\ of\ anemic\ patients\ is\ 297 .11 \ 46.95\ Co\ Units\ per\ 24 \– hour\ Urine,\ While\ that\ of\ the\ patients\ with\ protein \– energy\ malnutrition\ is\ only\ 187 .66\ \ 16.15\ Co\ Units\ per\ 24 \– hour\ Urine.\ In\ the\ case\ of\ anemic\ patients,\ the\ urinary\ erythropoietin level\ in\ patients,\ the\ \ urinary\ erythropietin\ level\ in\ aplastic\ anemia\ is\ the\ highest\ among\ the\ group,\ in\ which\ the\ average\ activity\ is\ equal\ to\ 725.94 88.31\ Co\ Units\ per\ 24 hour\ Urine.\
ABSTRACT
Abstract A periodate-resorcinol microassay for the quantitation of total sialic acid (TSA) has been developed. A 96-well microtiter plate format was used as a single reagent mixing well. The principle of this newly-developed assay is the periodate-resorcinol procedure. Conditions for the quantitation of sialic acid in serum, such as the concentration of periodate, resorcinol, incubation time and human serum volume, were optimized. It was found that the optimal concentration of periodic acid and resorcinol reagent were 1.3 mM and 0.6%, respectively. An incubation time of 60 minutes for the reaction of periodic acid and resorcinol with samples was found to give the highest absorbance. These new procedures were used for the quantitation of sialic acid in human serum. It was found that only 5 μL samples were needed to give a varied coefficient of the intraand inter-assay of 0.79% and 4.68%, respectively. Furthermore, this method demonstrated the recovery percentage of 94.25% by the addition of a known amount of pure sialic acid. This has been used to quantitate TSA in normal healthy and cancer serum and a significant difference between these two groups (p
ABSTRACT
Fifteen medicinal plant extracts were investigated for: total phenolic content and free radical scavenging effect by DPPH and ABTS assays; anti-lipid peroxidation activity by TBARS; and for antiglycation activity. The results revealed that the total phenolic content showed good correlation with free radical scavenging by ABTS [r = 0.721] and anti-lipid peroxidation by TBARS [r = -0.659], but showed no correlation with antiglycation. Three extracts from Tamarindus indica, Camellia sinensis and Artocarpus lakoocha demonstrated a significant antioxidant effect, and also showed a promising antiglycation effect. The IC50 [mg/ml] were 0.9-0.16 for the DPPH method; TEAC values [mg Trolox/mg sample] of 1.72-2.83 for the ABTS method; IC50 [mg/ml] of 0.64- 1.22 for the TBARS method; and IC50 ranging from 0.01 to 3.20 for the antiglycation method. These three herbs were found to possess effective antioxidant and antiglycation activities, and could be further developed for use in anti-aging cosmetics