ABSTRACT
Background: Non-obstructive azoospermia [NOA] occurs in approximately 10% of infertile men. Retrieval of the spermatozoa from the testicle of NOA patients is an invasive approach. Seminal plasma is an excellent source for exploring to find the biomarkers for presence of spermatozoa in testicular tissue. The present discovery phase study aimed to use metabolic fingerprinting to detect spermatogenesis from seminal plasma in NOA patients as a non-invasive method
Methods: In this study, 20 men with NOA were identified based on histological analysis who had their first testicular biopsy in 2015 at Avicenna Fertility Center, Tehran, Iran. They were divided into two groups, a positive testicular sperm extrac-tion [TESE[+]] and a negative testicular sperm extraction [TESE[-]]. Seminal plasma of NOA patients was collected before they underwent testicular sperm extraction [TESE] operation. The metabolomic fingerprinting was evaluated by Raman spec-trometer. Principal component analysis [PCA] and an unsupervised statistical meth-od, was used to detect outliers and find the structure of the data. The PCA was ana-lyzed by MATLAB software
Results: Metabolic fingerprinting of seminal plasma from NOA showed that TESE [+] versus TESE[-] patients were classified by PCA. Furthermore, a possible subdi-vision of TESE[-] group was observed. Additionally, TESE[-] patients were in ex-treme oxidative imbalance compared to TESE[+] patients
Conclusion: Metabolic fingerprinting of seminal plasma can be considered as a breakthrough, an easy and cheap method for prediction presence of spermatogenesis in NOA
ABSTRACT
Currently, there are 20,197 human protein-coding genes in the most expertly curated database [UniProtKB/Swiss-Pro]. Big efforts have been made by the international consortium, the Chromosome-Centric Human Proteome Project [C-HPP] and independent researchers, to map human proteome. In brief, anno 2017 the human proteome was outlined. The male factor contributes to 50% of infertility in couples. However, there are limited human spermatozoa proteomic studies. Firstly, the development of the mapping of the human spermatozoa was analyzed. The human spermatozoa have been used as a model for missing proteins. It has been shown that human spermatozoa are excellent sources for finding missing proteins. Y chromosome proteome mapping is led by Iran. However, it seems that it is extremely challenging to map the human spermatozoa Y chromosome proteins based on current mass spectrometry-based proteomics technology. Post-translation modifications [PTMs] of human spermatozoa proteome are the most unexplored area and currently the exact role of PTMs in male infertility is unknown. Additionally, the clinical human spermatozoa proteomic analysis, anno 2017 was done in this study
ABSTRACT
Transdiagnostic cognitive-behavioral treatments for emotional disorders are a new approach that empirically supported. Despite this most of the researches in this field have no control group and so there is a little information about comparing of the effect of transdiagnostic approach to others. The current study was compared transdiagnostic with cognitive-behavior therapy on patients with depression and anxiety disorders. This study was a pretest-post test randomized control trial. A diagnostically heterogeneous clinical sample of 23 patients with a principal depression and anxiety disorders that randomly assigned in two groups and participated in eight sessions. Participants were recruited from clinical psychology and psychiatry clinics of Taleghani hospital, Tehran, Iran. Beck Depression Inventory [BDI-II], Beck Anxiety Inventory [BAI], and Quality of life Scale [IRQL] were completed pre and post intervention by participants. The results were analyzed by dependent sample t-test and analyses of covariance [ANCOVA]. Data analyses showed that transdiagnostic therapy was effective for decreasing anxiety and depression symptoms, and have more effect for anxiety in comparing to cognitive-behavior therapy. Transdiagnostic therapy was confirmed in decreasing depressive and anxiety symptoms and improving quality of life in patients with depression and anxiety disorders and transdiagnostic therapy was more effective than cognitive-behavior therapy for decrease anxiety symptoms
ABSTRACT
The goal of this study was to examine the construct validity of the diagnostic and statistical manual of mental disorder-5 [DSM-5] conceptual model of antisocial and borderline personality disorders [PDs]. More specifically, the aim was to determine whether the DSM-5 five-factor structure of pathological personality trait domains replicated in an independently collected sample that differs culturally from the derivation sample. This study was on a sample of 346 individuals with antisocial [n = 122] and borderline PD [n = 130], and nonclinical subjects [n = 94]. Participants randomly selected from prisoners, out-patient, and in-patient clients. Participants were recruited from Tehran prisoners, and clinical psychology and psychiatry clinics of Razi and Taleghani Hospital, Tehran, Iran. The SCID-II-PQ, SCID-II, DSM-5 Personality Trait Rating Form [Clinician's PTRF] were used to diagnosis of PD and to assessment of pathological traits. The data were analyzed by exploratory factor analysis. Factor analysis revealed a 5-factor solution for DSM-5 personality traits. Results showed that DSM-5 has adequate construct validity in Iranian sample with antisocial and borderline PDs. Factors similar in number with the other studies, but different in the content. Exploratory factor analysis revealed five homogeneous components of antisocial and borderline PDs. That may represent personality, behavioral, and affective features central to the disorder. Furthermore, the present study helps understand the adequacy of DSM-5 dimensional approach to evaluation of personality pathology, specifically on Iranian sample
Subject(s)
Humans , Male , Female , Borderline Personality Disorder/diagnosis , Mental Disorders , Personality , Reproducibility of ResultsABSTRACT
Infertility is one of the most serious social problems. Illicit drug use can be an important cause of male factor infertility. Kerack which its use is rising up in Iran refers to a high purity street-level heroin [heroin Kerack]. Heroin Kerack used in Iran is an opioid and has harmful effects on body organs. The aim of this study is to investigate the effects of Kerack used in Iran on fertility adult mice. In this study, 25 male mice were divided into five groups [control, sham and three experimental]. Experimental groups of Kerack-dependent mice [received ascending dose of Kerack for seven days] were divided into three categories, experimental I, II and III. Experimental I was given Kerack at a dose of 5 mg/kg, experimental II 35 mg/kg and experimental III 70 mg/kg, intraperitoneally twice a day for a period of 35 days. The sham group received normal saline and lemon juice [2.6 micro l/ml] whilst the control group just received water and food. Mice were then scarified and sperm removed from cauda epididymis were analyzed for sperm count, motility, morphology [normal/abnormal] and viability. Testes were also removed, weighed and processed for light microscopic studies. The results showed that fertility were significantly decreased in addicted mice compared with control groups [P