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Objective:To determine the seroprevalence of celiac disease in susceptible population, and to analyze the relationship between demographic characteristics, dietary habits, lifestyle and serological positivity so as to provide guidance for the prevention and treatment of celiac disease in Southern China.Methods:A total of 1 273 individuals who participated in Guangdong Province Health Screening Program in 2015, were selected as serologically positive subjects of celiac disease, including people with irritable bowel syndrome, colitis, diarrhea, anemia, low BMI, short stature, type 1 diabetes mellitus (T1DM), rheumatoid arthritis (RA), ankylosing spondylitis, psoriasis and bristol grade=6 or 7. All subjects were tested for serum IgA anti-tissue transglutaminase antibodies (TTGA), IgA antibodies against deamidated gliadin peptides(DGPA) and IgG against deamidated gliadin peptides (DGPG). Dietary habits, lifestyle and demographic characteristics were compared in subgroups.Results:The seroprevalence of celiac disease in susceptible population was 0.94% (95% CI 0.54%-1.64%) including 0.08% (1/1 273) for TTGA, 0.47% (6/1 273) for DGPA, and 0.39% (5/1 273) for DGPG. The seropositive rate was 3.6% (1/28) in patients with psoriasis, 2.1% (2/95) in the low BMI group, 1.9% (1/53) in T1DM group, 1.8% (3/169) in diarrhea group and 1.1% (5/463) in RA group. No significant difference was found in age, gender, high carbohydrate diet or lifestyle between the negative and the positive subjects. Conclusions:In Southern China, the seropositive rate of celiac disease is 0.94% in susceptible population, which prompts an urgent need of serological screening for early diagnosis.
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Objective To investigate the expression changes of histidine decarboxylase(HDC)and H+,K+-ATPase in gastric mucosa during the healing of experimental gastric ulcer in rats.Methods The ulcers were caused by applying acetic acid to the serosal surface of the anterior face of the rat gastric body.At different time points during ulcer healing,HDC and H+,K+-ATPase mRNA and protein expressions were studied by using reverse transcription-polymerase chain reaction and Western blot respectively.Results An ulcer crater developed on the anterior face of the gastric body on day 1 after the induction of ulcers,and the ulcer area was biggest on day 3.On day 12,most of the gastric ulcers had healed.Compared with the control group,the HDC and H+,K+-ATPase mRNA expression in the gastric mucosa of ulcer rats showed a decrease on day 1,and increased back to initial level on day 9.The protein expression of HDC and H+,K+-ATPase in gastric mucosa of ulcer rats decreased immediately on day 1,more on day 6,and returned to the initial levels on day 12.Conclusion The mRNA and protein expressions of HDC and H+,K+-ATPase decrease in the healing process of gastric ulcers,resulting in accelerated ulcer healing through inhibiting gastric acid secretion.
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Objective:To investigate the role of PUMA,a Bcl-2 family member,in oxaliplatin-induced apoptosis in colorectal cancer cells.Methods:In vitro,LoVo cells were treated with 1.2,2.4,4.8 ?mol/L oxaliplatin for 24 h and 4.8 ?mol/L oxaliplatin for 6,12,24 h,PUMA expression was assessed by Western blot analysis.The anti-sense PUMA plasmid was constructed by gene recombination.PUMA expression was suppressed by stable transfecting anti-sense PUMA plasmid into LoVo cells.The apoptosis was assessed by flow cytometry analysis(FCM) and proliferation rate was assessed by MTT assay.Results:Oxaliplatin induced PUMA expression in a time-and dose-dependent manner;stable cell lines expressing the anti-sense PUMA vector were established and named LoVo puma(-/-) cells;oxaliplatin-induced apoptosis was decreased by suppression of PUMA expression;the proliferation rate in LoVo puma(-/-) cells was higher than that in LoVo cells after oxaliplatin treated.Conclusion:Our data suggest that PUMA plays an important role in oxaliplatininduced apoptosis in LoVo cells and the sensitivity of LoVo cells to oxaliplatin was abrogated by suppression of PUMA expression.
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Objective To study the relationship between gastric emptying dysfunction and neurotensin(NT) in functional dyspepsia(FD) patients. Methods Gastric emptying function in 54 FD patients was measured with solid radioopaque markers. Patients were divided into two groups according to the results, one of which with delayed gastric emptying and the other with normal gastric emptying. Seventeen healthy volunteers served as normal control group. The NT levels in plasma (fasting and postprandial) and the mucosa of gastric antrum and descending portion of duodenum were measured by radioimmunoassay(RIA). Results The NT levels in fasting and postprandial plasma and the mucosa of gastric antrum and descending portion of duodenum in FD group with delayed gastric emptying were significantly higher than those in FD group with normal gastric emptying and in normal control group. The three groups did not differ significantly in incremental extent of plasma NT after test meal. The NT levels in fasting and postprandial plasma and the mucosa of gastric antrum and descending portion of duodenum in FD group with normal gastric emptying did not differ statistically from those in normal control group. Conclusion Gastric emptying dysfunction is closely related to NT, which can inhibit gastrointestinal motility and delay gastric emptying. Thus, NT is important in the pathogenesis of FD.
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Objective To study the relationship between gastrointestinal hormones and gastric emptying in functional dyspepsia (FD) patients. Methods Gastric emptying was assessed with solid radiopaque markers in 54 patients with functional dyspepsia, and then all patients were divided into two groups, one with delayed gastric emptying and the other with normal gastric emptying. 17 healthy volunteers served as normal control group. The plasma NT, MTL, Gas and SS levels (fasting and 30 minutes after meal) were measured by radioimmunoassay (RIA). Results The fasting and postprandial plasma NT levels and the postprandial plasma Gas level were significantly higher in FD group with delayed gastric emptying than those in FD group with normal gastric emptying and in normal control group. The MTL levels in fasting and postprandial plasma were significantly lower in FD group with delayed gastric emptying than those in FD group with normal gastric emptying and in normal control group. The plasma SS levels did not show differences among three groups. Conclusion Gastrointestinal dysmyotonia is closely related to NT, MTL and Gas. NT, MTL and Gas may be important in the pathogenesis of FD.
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Objective To investigate the effects of Celecoxib,a selective COX-2 inhibitor,on the healing of gastric ulcer in rats,and reveal the rale of ERK signal transduction pathway in the mechanism of delaying the healing of gastric ulcer.Methods Forty-eight rats were randomly divided into model group(n=40) and sham operation group(n=8).In the rats in model group acetic acid-induced gastric ulcer was reproduced,while in sham operation group,rats underwent the sham procedure.Eight rats in sham-operation group and eight rats in model group were euthanized three days after the procedure.The other thirty-two rats in model group were divided into two subgroups including Celecoxib group and NS group(n=16).Three days after the procedure,rats in Celecoxib group received a gavage of 0.2% Celecoxib solution,and those in NS group received equal amount of 0.9% NaCl solution.Rats in Celecoxib group and NS group were euthanized on sixth and ninth day after ulcer induction(8 rats at each time point in each group).The effects of Celecoxib on the healing of gastric ulcer were observed.Its effects on the activity of Raf-1 and ERK1/2,and the expression level of two transcription factors c-Fos and c-Jun were also determined by Western blot analysis.Results Nine days after ulcer induction,the ulcer area was 11.9?3.1mm2 and 19.7?3.8mm2 in NS group and Celecoxib group,respectively,and they were much smaller than those on third day(P