The role of SBAR communication model combined with scenario simulation in clinical teaching of blood purification center / 中华医学教育探索杂志

Objective:To explore the effect of SBAR (situation, background, assessment, recommendation) communication model combined with scenario simulation in clinical teaching of blood purification center.Methods:Sixty interns in the blood purification center from March 2019 to March 2020 were collected in the study, and randomly divided into experimental group ( n=30) and control group ( n=30). The two groups of students studied in two different treatment groups in the blood purification center. The control group used the method of traditional teaching, while the experimental group used SBAR communication combined with situational simulation for teaching on the basis of traditional teaching, with a total clinical practice time of two months. All trainees underwent theoretical examination, clinical operation examination, self-efficacy evaluation and teaching satisfaction survey after training. SPSS 22.0 was performed for t test. Results:Through SBAR communication model combined with situational simulation teaching, the scores of theoretical examination [(88.13±3.22) vs. (85.51±3.17)], clinical operation assessment [(91.31±3.48) vs. (84.17±4.94)], self-efficacy evaluation [(11.13±1.08) vs. (9.21±1.89)], teaching satisfaction [(95.71±2.87) vs. (91.18±3.08)], and communication ability [(12.27±1.13) vs. (8.63±1.45)] in the experimental group were higher than those in the control group; while there was no significant difference in the scores of theoretical assessment between the two groups ( P<0.05). Conclusion:SBAR communication model combined with situational simulation teaching can improve the learning ability, operation ability and comprehensive application ability of special training trainees, especially in communication ability, which is worthy of popularization and application in clinical teaching.

Research progress on protective effect of traditional Chinese medicine and natural medicine on methotrexate-induced liver toxicity / 中国中药杂志

Methotrexate(MTX) is a commonly used antimetabolite, which can be used in the treatment of a variety of diseases. However, hepatotoxicity in the use of MTX severely limits its clinical use. Therefore, how to prevent and treat hepatotoxicity of MTX has become an urgent clinical problem. This paper summarizes and analyzes relevant literatures on the prevention and treatment of hepa-totoxicity caused by MTX with traditional Chinese medicines and natural medicines in recent years. MTX-induced hepatotoxicity mechanisms include folate pathway, oxidative stress damage and adenosine pathway, of which oxidative stress theory is the main research direction. A total of 14 kinds of traditional Chinese medicine and natural medicine extracts including white peony root, and 21 kinds of natural monomer compounds, including berberine, play an anti-MTX-induced hepatotoxic effect by resisting oxidative stress, inhibiting inflammation and regulating signal pathways. According to current studies on the prevention and treatment of hepatotoxicity induced by MTX with traditional Chinese medicines and natural medicines, there are insufficiencies, such as partial and superficial mechanism studies, inadequate combination of experimental research and clinical practice, non-standard experimental design and lack of application of advanced technologies and methods. This paper systematically reviewed the effects and mechanisms of traditional Chinese medicines and natural medicines against hepatotoxicity induced by MTX and defined current studies and deficiencies, in the expectation of proposing new study strategies and directions and providing scientific basis for rational clinical use of MTX and development of new drugs against MTX hepatotoxicity.

Inhibitory Effect of Weichang'an Granule on Invasion and Metastasis of MKN45 Cells in Human Gastric Cancer by RUFY3 / 中国实验方剂学杂志

Objective:To explore the molecular mechanism of spleen-strengthening traditional Chinese medicine （TCM） Weichang'an granule in inhibiting the invasion and metastasis of human gastric cancer MKN45 cells. Method:MKN45 cells were cultured <italic>in vitro</italic> and incubated with different concentrations（600， 900， 1 200， 1 500 mg·L^-1）of Weichang'an granule for 24， 48， 72 h. Cell counting kit-8 （CCK-8） assay was used to detect its effect on the cell proliferation. Western blot was used to detect the expression of RUN and FYVE domain containing 3（RUFY3） in normal gastric mucosa cells and different gastric cancer cell lines. The expression of RUFY3 in the gastric cancer cells after Weichang'an granule intervention （600， 900， 1 200 mg·L^-1） was detected by Western blot. Lentivirus transfection technique was used to achieve the stable and silenced expression of RUFY3 in gastric cancer MKN45 cells. Transwell assay was used to evaluate the influence of Weichang'an granule and silenced RUFY3 on the metastasis and invasion ability of MKN45. E-cadherin，N-cadherin，Vimentin，Zinc-finger transcription factor （SNAIL1 and SNAIL2） protein expression levels were detected by Western blot. Result:RUFY3 expression in human gastric cancer cells was significantly higher than that in normal gastric mucosa．The protein expression of RUFY3 in MKN45 cells of silenced RUFY3 group was significantly lower than that in Lentivirus negative group （<italic>P</italic><0.01）． Weichang'an granule inhibited the expression of RUFY3 in human MKN45 gastric cancer cells （<italic>P</italic><0.05， <italic>P</italic><0.01） in a concentration-dependent manner. As compared with the blank group， both Weichang'an granule and silenced RUFY3 inhibited the metastasis and invasion ability of MKN45 （<italic>P</italic><0.01）. After Weichang'an granule and silenced RUFY3 treatment， the protein expression of epithelial marker gene E-cadherin was up-regulated （<italic>P</italic><0.01）， the protein expression of Vimentin and N-cadherin decreased， but with no statistical difference，while SNAIL1 and SNAIL2 were both significantly down-regulated （<italic>P</italic><0.01）. Conclusion:By targeting RUFY3 to regulate epithelial mesenchymal transformation， the spleen-strengthening TCM compound Weichang'an granule can inhibit the invasion and metastasis of gastric cancer.

Effect of methyl eugenol on hypoxia/reoxygenation injury of human renal tubular epithelial cells and its mechanism / 中国中药杂志

This study aimed to investigate the effect of methyl eugenol(ME) on hypoxia/reoxygenation(H/R)-induced injury of human renal tubular epithelial HK-2 cells and its mechanism. The viability of HK-2 cells cultured with different concentrations of ME and exposed to H/R was detected by cell counting kit-8(CCK-8) assay. The effect of ME on the morphology of HK-2 cells was observed under an inverted microscope. The content of intracellular reactive oxygen species in different groups was detected after 2',7'-dichlorodihydrofluorescein diacetate(DCFH-DA) fluorescence staining. Cell apoptosis was determined by flow cytometry. Changes in mitochondrial membrane potential were monitored by JC-1 dye. The concentrations of nuclear factor erythroid 2 related factor 2(Nrf2), heme oxygenase-1(HO-1), and nicotinamide adenine dinucleotide phosphatase oxidase 4(Nox4) were measured by Western blot, followed by the assay of Nrf2 concentration changes in cytoplasm and nucleus by confocal fluorescence staining. The results showed that when the concentration of ME was 0-40 μmol·L~(-1), the activity of HK-2 cells was not affected. Compared with the model group, ME enhanced the activity of HK-2 cells and the cell morphology was normal. As revealed by further experiments, ME inhibited the release of reactive oxygen species and the decline in mitochondrial membrane potential of HK-2 cells after H/R injury, promoted Nrf2/HO-1 expression and Nrf2 translocation to the nucleus, and down-regulated the expression of Nox4, thereby significantly reducing apoptosis. This protective effect of ME could be reversed by the specific Nrf2 inhibitor ML385. These findings have preliminarily proved that ME effectively protected HK-2 cells against H/R injury, which might be related to its promotion of Nrf2/HO-1 signaling pathway and inhibition of Nox4. Such exploration on the possible mechanism of ME in the treatment of renal ischemia-reperfusion injury(IRI) and protection of organ function from the perspective of antioxidant stress has provided reference for related research on the treatment of acute kidney injury with traditional Chinese medicine.

Application of Magnetization-prepared True Fast Imaging with Steady-state Precession Sequence in Brain Tumor Enhancement / 中国医学科学院学报

Objective To evaluate the application of two-dimensional magnetization-prepared true fast imaging with steady-state precession(2D-MP-TrueFISP)sequence in brain tumor enhancement.Methods In this study,60 cases of brain tumor patients who underwent enhanced magnetic resonance imaging of brain were scanned with 2D-MP-TrueFISP/two-dimensional spoiled gradient-recalled echo(2D-SPGR)before and after enhancement.The scores of lesions on the images of 2D-MP-TrueFISP/2D-SPGR were compared.At the same level of 2D-SPGE and 2D-MP-TrueFISP,the signal intensities(SIs)of lesions,white matter,and cerebrospinal fluid were measured before and after enhancement,and the contrast ratios(CRs)of lesions were calculated.The CRs before and after 2D-SPGR/2D-MP-TrueFISP enhancement and those between 2D-SPGR and 2D-MP-TrueFISP after enhancement were compared.Results The scores of lesions after 2D-MP-TrueFISP/2D-SPGR T1WI enhancement were 9.0(9.0,9.0)and 7.0(6.0,7.0),respectively,with significant difference(

Mechanism of Weichang' an on Autophagy of MKN45 Cells / 中国实验方剂学杂志

<b>Objective::To explore the effect of Weichang' an (WCA) on the autophagy of human gastric cancer MKN45 cells and its possible anti-cancer mechanism. <b>Method::MKN45 cells were cultured <italic>in vitro</italic> and incubated with different concentrations (250, 500, 1 000, 2 000 mg·L^-1) of WCA for 24, 48, 72 h. Cell counting kit-8 (CCK-8) assay was used to detect the cell proliferation. AO/EB Dyeing (AO) staining and monodansylcadaverin (MDC) staining were used to observe the changes of the effect of WCA on autophagosome and autophagic vesicles in gastric cancer MKN45 cells at 48 h. Real-time polymerase chain reaction (Real-time PCR) and Western blot were used to detect microtubule-associated protein 1 light chain 3 (LC3), Beclin1, sequestosome 1 (p62), human autophagy-related gene 5 (ATG5), human autophagy-related gene 7 (ATG7) mRNA and protein expression levels. <b>Result::WCA showed a dose-and-time-dependent growth inhibition at the concentration above 1 000 mg·L^-1. Compared with the blank group, WCA (500, 1 000, 2 000 mg·L^-1) significantly inhibited the proliferation of MKN45 cells (<italic>P</italic><0.05, <italic>P</italic><0.01). AO staining and MDC staining showed that autophagosomes and autophagic sacs increased with the rise of WCA concentration compared with the blank group. Real-time PCR and Western blot showed that the expressions of autophagy-related proteins LC3-Ⅱ, Beclin1, ATG5, ATG7, mRNA and protein increased gradually after WCA (1 000 mg·L^-1) intervention, while p62 expression decreased (<italic>P</italic><0.05, <italic>P</italic><0.01). <b>Conclusion::WCA induces the autophagy of human gastric cancer MKN45 cells in a time-and dose-dependent manner <italic>in vitro</italic>, which may be related to the up-regulation of LC3-Ⅱ, Beclin1, ATG5 and ATG7 as well as the down-regulation of p62 and LC3-Ⅰ.

Socio-cultural factors influencing the buying commercial homosexual sex behaviors among young homosexual/bisexual men / 中华疾病控制杂志

Objective To investigate the buying commercial homosexual sex behaviors among young gay men/bisexual men, and analyze the socio-cultural factors influencing the buying commercial homosexual sex behaviors. Methods A cross-sectional study was performed on risk sexual behaviors for acquired immunodeficiency syndrome (AIDS) with an anonymous questionnaire among 824 young homosexual/bisexual men in Guiyang City, Zunyi City, Anshun City and Tongren City of Guizhou Province in 2016. Respondents were selected by a classified snowball sampling method combined with an interview at AIDS voluntary consultation and testing clinics. Results About 7.8%(95% CI：5.9%-9.6%)of respondents reported the buying commercial homosexual sex behaviors in the last year. The report rate was 13.0% for homosexual men, higher than 5.7% for bisexual men (P＜0.001). The influencing factors of the buying commercial homosexual sex behaviors were average monthly income (OR=6.54, P=0.005), age (OR=3.59, P=0.011), age of liking the same-sex (OR=2.85, P=0.004), marital status (OR=2.62, P=0.005), household registration (OR=2.38, P=0.011), increased risk of oral sex (OR=0.42, P=0.019), possibility of HIV infection (P＜0.005), and the dusckisure of sexual orientation (OR=0.17, P=0.001). Conclusions Young homosexual/bisexual men, especially bisexual men, had a higher report rate of the buying commercial homosexual sex behaviors. The socio-cultural factors influencing the buying commercial homosexual sex behaviors included age of liking the same-sex, increased risk of oral sex, possibility of HIV infection, and the disclosure of sexual orientation.

Progress in the therapeutic use of methylene blue in ischemic stroke / 解剖学报

Stroke has been widely studied as a highly lethal and highly disabling disease worldwide. Methylene blue approved by the Food and Drug Administration (FDA) of the United States has been used in the treatment of methemoglobinemia and cyanide poisoning for more than one hundred and twenty years. In recent studies, it has been found to have neuroprotective effects and is effective in a few of animal models of ischemic stroke. This article briefly reviews the recent research on the therapeutic use of methylene blue in ischemic stroke.

A retrospective study of diabetes in middle-aged and elderly urban population in Hainan, south China

Objective:To investigate the distribution characteristics and epidemic trends of diabetes in middle-aged and elderly urban Hainanese, and to explore the correlations between diabetes and hypertension or hyperlipidemia in this kind of people.Methods:Middle-aged and elderly urban Hainanese (35–74 years old) were examined at the Medical Center of the Second Affiliated Hospital of Hainan Medical University from 2013 to 2017. According to the World Health Organiza-tion (WHO) Diabetes Diagnostic Criteria 1999, subjects with fasting blood glucose (FBG) level ⩾7.0 mmol/L (126 mg/dL) were diagnosed as diabetes. The retrospective analysis of the collected cleared data was conducted by using chi-square test, single factor correlation analysis and multiple linear regression analysis methods [gender: X1 (male=1, female=2); age: X2 (35–44=1, 45–54=2, 55–64=3, 65–74=4). The test level was set as 0.05. All data were standardized by age according to the 6th National Census Data 2010.Results:A total of 69 077 middle-aged and elderly observations’ data in 2013–2017 were collected. The detection rates of diabetes were 8.28%, 8.89%, 10.71%, 8.29%, and 9.21% respectively by year (χConclusions:The detection rate of diabetes in middle-aged and elderly urban Hainanese increased in the past five years and it was linearly related with gender and age; weak correlations between diabetes and hypertension or hyperlipidemia were observed. Further prospective studies should be done in the future.

The domestic hotspots research of formative assessment based on the knowledge map analysis / 中华医学教育探索杂志

Objective To summarize and analyze the present situation, hotspot and development trend of the research and practice about the formative assessment in Chinese medical teaching reform by bibliometrics, and provide a quantitative method for the study of formative assessment. Methods We took 567 journals about formative assessment from CNKI database as the research object, making co-word clus-tering and multidimensional scaling analysis as the main research method, and using Bicomb co-word anal-ysis software and SPSS to complete content data statistics and analysis. Result The knowledge map of formative assessment research can be divided into 7 fields: field 1 shows that most studies are mainly focused on practical research, the effectiveness evaluation on application are mainly focused on the improve-ment of students' independent learning ability. The proportion of research in traditional Chinese medicine colleges or universities is increasing, researches of clinical teaching take an important position, and nursing educations are paying more attention as well. Field 2 and field 4 show the synchronization between assess-ment reform and teaching content/strategy reform. Field 3 presents the general situation of teaching evaluation research in higher vocational colleges. Field 5 shows the theme of exploring the establishment of a formative assessment system . Field 6 shows the differences and similarities between examination and assessment . Field 7 shows the overview of medical English courses. Conclusion In the future, we should actively carry out the construction and exploration of the formative assessment system that conforms to China's national conditions, and attach importance to the construction of the sharing platform. We should avoid duplication of research , attach importance to the study and understanding of theoretical connotation and strengthen training, supervision and management.

MMP26: A Potential Biomarker for Prostate Cancer / 华中科技大学学报（医学）（英德文版）

The application of prostate-specific antigen (PSA) in the screening and diagnosis of prostate cancer (PCa) has improved the clinical management of PCa patients.However,the PSA assay has been faced with criticism due to its potential association with over-diagnosis and subsequent overtreatment of indolent patients.Matrix metalloproteinase-26 (MMP26) is a member of matrix metalloproteinases (MMPs) and has been reported to be highly expressed in many cancers.This investigation evaluated the potential of serum MMP26 as a biomarker for PCa.The level of serum MMP26 was measured by enzyme-linked immunosorbent assay (ELISA) in 160 subjects including PCa group (n=80),benign prostatic hyperplasia (BPH) group (n=40) and control group (n=40).Furthermore,we evaluated the expression of MMP26 in tissues by immunohistochemistry.The results showed the serum MMP26 levels were significantly higher in PCa group than in BPH group and control group.Similarly,the MMP26 protein was positive in PCa tissues and negative in BPH tissues and control tissues.In conclusion,these results suggested MMP26 could be used as a potential serum biomarker in the diagnosis of PCa.

Regional homogeneity of brain in irritable bowel syndrome patient:a resting state fMRI study / 实用医学杂志

Objective To investigate regional homogeneity(ReHo)of brain in patients with irritable bowel syndrome(IBS)using resting-state functional MRI(rs-fMRI). Methods 23 patients with IBS and 23 age-,edu-cation-,and sex-matched normal healthy subjects(control group)were collected in our hospital from July 2015 to June 2016. All of them underwent rs-fMRI. The rs-fMRI data were analyzed with the regional homogeneity method. Results As compared with the control group ,the IBS group showed significantly increased ReHo values in the left occipital lobe,and decreased ReHo values in the right insular,the right superior temporal gyrus and bilateral middle frontal gyrus(P < 0.001). Conclusion One or more brains of the IBS patients showed significant differ-ences in the spontaneous neuronal activity compared with healthy controls.

Studies on the characteristics and cytotoxicity of gentamicin/chitosan microspheres for local injection / 中国生化药物杂志

Objective To construct the drug delivery system of gentamicin/chitosan microspheres for local injection, and evaluate its physicochemical properties and cell cytotoxicity.Methods Gentamicinwas used as model drug, chitosan as carrier, lecithinand hydroxypropyl-β-cyclodextrin as accessories, and the microspheres of gentamicin/chitosan/lecithin/hydroxypropyl-β-cyclodextrinwas prepared by spray drying method.The physicochemical properties and cell cytotoxicity of themicrospheres were investigated by UV spectrophotometry , scanning electron microscopy, X-ray diffraction, dynamic membrane dialysis and MTT assay.Results Five kinds of chitosan microspheres ( A, B, C, D and E) with different drug/carrier ratios were successfully prepared by spray drying method.The yield, drug loading and entrapment efficiency of the drug-loaded microspheres were 34.38%~46.94%, 10.20% ~18.67%, 61.20% ~74.72%, respectively.SEM results showed that compared with microspheres A, B and C, microspheres D and E own the spherical shape with wrinkled surface and uniform particle size, particle size between 0.5 ~3 μm, no adhesion.X-ray diffraction analysis showed that the drug was encapsulated in the microspheres.The results of in vitro release indicated that microspheres D had a good sustained release effect in the four drug-loaded microspheres.The results of cytotoxicity test showed that when the concentration of gentamicin reached 400 μg/mL, the relative growth rate of microspheres D was still higher than 80% with the cytotoxicity grade was one, ie, no cytotoxicity.Conclusion The microspheres D with good physicochemical properties, sustained-release effect and biocompatibility, is expected to be a good carrier of prostate local drug delivery.

Microbial Transformation of Glycosides in Chinese Herbal Medicine / 世界科学技术-中医药现代化

Glycosides are the active ingredients (AIs) of many Chinese herbs and have become hot spots along with the findings of their new functions,such as anti-inflammatory,antivirus,enhanced immunity and anti-cancer.It has been found that glycosides exert their effects by converting to aglycons or other AIs in vivo.Therefore,the transformation of glycosides to the corresponding AIs in vitro becomes very important to enhance their bioavailabilities.The microbial transformation has an unparalleled advantage in the transformation of Chinese herbs in vitro for its reaction specificity,less by-products,mild reaction conditions and environmental protection.This paper summarized and prospected researches of glycosides' microbial transformation.

Analysis of coagulation related parameters between patients with advanced schistosomiasis cirrhosis and hepatitis B cirrhosis / 中国血吸虫病防治杂志

Objective To analyze the differences of coagulation parameters between patients with advanced schistosomiasis cirrhosis and hepatitis B cirrhosis under different degrees of liver function,so as to provide the evidence for further guidance of judging clinical condition and prognosis. Methods Sixty?three patients with advanced schistosomiasis cirrhosis and eighty pa?tients with hepatitis B induced cirrhosis hospitalized in Jingzhou Central Hospital from January 2014 to June 2016 were served as an advanced schistosomiasis cirrhosis group and a hepatitis B cirrhosis group,respectively,and ninety?six gastropathy pa?tients excluded from other diseases that might affect coagulation in the same period were served as a control group. The levels of PT,INR,Fib,TT,APTT,and PLT of the patients among the above 3 groups and the cirrhosis patients with different Child?Pugh classes were detected and compared. Results The differences of the levels of PT,INR,Fib,TT,APTT,and PLT among the 3 groups were statistically significant(F=84.512,81.672,37.612,104.475,52.497,102.233;all P 0.05). Under all the grades of liver function,TT and APTT of the hepatitis B induced cirrhosis group was longer than those of the advanced schistosomiasis cirrhosis group ,and the Fib level of the former was lower than that of the latter. Among those with the liver function of Grade A and B ,PT and INR of the hepatitis B induced cirrhosis group were longer than those of the advanced schistosomiasis cirrhosis group;while for those with the liver function of Grade C,the difference between the two groups was not statistically significant(all P > 0.05). Conclu?sions There exist differences of the damage degrees of coagulation function between the patients with advanced schistosomiasis cirrhosis and hepatitis B cirrhosis. When the damage degree of the liver function is lighter,the coagulation function in the pa?tients with hepatitis B cirrhosis decreases more significantly;when the damage degree becomes more severe,APPT in the pa?tients with hepatitis B cirrhosis extends more significantly,while the differences of PLT and PT between them are little.

Regional homogeneity of brain in irritable bowel syndrome patient:a resting state fMRI study / 实用医学杂志

Objective To investigate regional homogeneity(ReHo)of brain in patients with irritable bowel syndrome(IBS)using resting-state functional MRI(rs-fMRI). Methods 23 patients with IBS and 23 age-,edu-cation-,and sex-matched normal healthy subjects(control group)were collected in our hospital from July 2015 to June 2016. All of them underwent rs-fMRI. The rs-fMRI data were analyzed with the regional homogeneity method. Results As compared with the control group ,the IBS group showed significantly increased ReHo values in the left occipital lobe,and decreased ReHo values in the right insular,the right superior temporal gyrus and bilateral middle frontal gyrus(P < 0.001). Conclusion One or more brains of the IBS patients showed significant differ-ences in the spontaneous neuronal activity compared with healthy controls.

Preparation and in vitro anticancer activity of DOX-VES-loaded copolymer micelles / 中国药学杂志

OBJECTIVE: To enhance the anticancer activity of doxorubicin(DOX) by conjugating DOX and vitamin E succinate (VES) and loading the conjugate into hyaluronic acid-octadecylamine (HA-C18) copolymer micelles. METHODS: DOX and VES were conjugated by amide reaction. DOX-VES/HA-C18micelles were prepared via a probe-type ultrasonication technique. The morphology of the micelles was determined using a transmission electron microscopy (TEM).Dynamic light scattering (DLS) technique was used to determine the particle size distribution, hydrodynamic diameters, and stability of the micelles. Ultracentrifugation was exploited for measuring the drug loading (DL) and encapsulation efficiency (EE), and the in vitro release was investigated using a dialysis tubing. The cellular uptake and cellular distribution of drug-loaded micelles in MCF-7 cells were observed by fluorescence microscope, and the fluorescence intensity of DOX was evaluated by flow cytometer. The cytotoxicity of free DOX and drug-loaded micelles was tested by MTT assay against MCF-7 cells. RESULTS: DOX-VES/HA-C18 showed a nearly spherical morphology and good stability in PBS (pH 7.4) and 10% FBS. The particle size and zeta potential were (184.6±9.42) nm and (-20.7±1.23) mV, respectively. The DL and EE were (15.8±2.85)% and (94.2±1.32)%, respectively. DOX-VES/HA-C18had a good controlled drug release property. Furthermore, DOX-VES/HA-C18with accumulation in nucleipresented higher anti-tumor activity than free DOX and DOX/HA-C18. CONCLUSION: DOX-VES conjugate has synergistic anti-tumor effect and good application prospects.

Preparation, characterization and antioxidation activity in vitro of quercetin loaded chitosan nanoparticles / 中国生化药物杂志

Objective To prepare quercetin ( QUE) loaded chitosan nanoparticles ( CS-NPs), evaluate its physicochemical properties and antioxidation activity in vitro.Methods Quercetin chitosan nanoparticles were prepared by ionic crosslinking method and self-assembly method.The preparation method was optimized using entrapment efficiency (EE), drug loading (DL) and size as indexes.The best formulation and preparation conditions were optimized by orthogonal test based on single-factor test, evaluation indicator as particle size and EE.The physicochemical properties of the obtained QUE-CS-NPs were characterized by the following methods: the transmission electron microscope (TEM), dynamic light scattering (DLS) analysis for morphology, size distribution and Zeta potential.In vitro release behavior in 0.5% SDS solution was evaluated by dialysis tube method.In vitro antioxidant activity assays were performed by evaluating the abilities of the microspheres for hydroxide radicals and superoxide anions .Results TEM results revealed QUE-CS-NPs with round and uniform.Particle-size analysis showed that the diameters and Zeta potential of the QUE-CS-NPs were (282.9 ±20) nm and (30.5 ±2) mV, with uniform distribution (polydispersity below 0.185).DL and EE of QUE-CS-NPs were (8.81 ±0.65) %and (80.02 ±1.04) %, respectively.QUE-CS-NPs showed extended administration times with 66.2% cumulative release within 72 h.QUE-CS-NPs showed pronounced antioxidant activity and a concentration dependent, even more substantial than that of pure QUE.Conclusion QUE-CS-NPs show a good size, sustain release effect and pronounce antioxidant activity.

Analysis of the relation between dental arch size and upper airway morphology in patients with obstructive sleep apnea hypoventilation syndrome / 实用口腔医学杂志

Objective:To study the anatomical correlation between dental arch and the volume of upper airway in patients with obstruc-tive sleep apnea hypoventilation syndrome(OSAHS). Methods: Dental arch architecture and upper airway volume were measured by cone beam CT(CBCT) in the subjects with OSAHS(n=22) and without OSAHS(n=19). The correlation between dental arch and the supper airway volume in OSAHS patients was analyzed. Results:The length of the upper dental arch and the height of palate in OSAHS patients were larger than those of the controls(All, P<0. 05). Cross-sectional area of nasopharynx and retropalatal and the total volume of upper airway were negatively correlated with the palatal height and upper dental arch length(P<0. 05), while positively correlated with upper dental arch of molar regions(P<0. 05). Conclusion:The abnormal shape of upper dental arch is related to the airway vol-ume of nasopharynx and retropalatal region in patients with OSAHS.

Retrospective analysis of craniofacial features in patients with cleidocranial dysplasia / 实用口腔医学杂志

Objective:To investigate the craniofacial features in patients with cleidocranial dysplasia (CCD).Methods:The facial features,cervical vertebral bone age and skeletal abnormalities of 8 patients with CCD were studied by analyzing facial photos,cephalo-metric and panoramic radiographs.Results:4 patients were in the early growth stage and the other 4 in the late period of development. The bossing forehead and inclined eye fissure were observed in all patients,but underdevelopment of midfaces were not obviously pres-ented in younger patients.Morphological abnormalities of craniofacial bones,such as ascending ramus,coronoid process,nasal bones and disappearence of gonial angle were observed in all patients.Conclusion:Some craniofacial malformations in patients with CCD may be presented earlier than underdeveloped midface,which can be helpful for early diagnosis of CCD.