ABSTRACT
Aim To investigate the effect of T40 on malignant biological behavior of glioma U87 and U251 cells and its mechanism. Methods U87 and U251 cells were treated with T40 at different concentrations (0,1,2 and 4 p,mol • L"1). Changes of cell proliferation, clonal formation, apoptosis, migration and invasion in each group were detected by CCK-8, cloning plate, flow cytometry, scratch and transwell experi-ments. Bioinformatics was used to explore T40 targets and analyze the relationship between targets and glioma progression. The protein expression levels of PTPN1, PTPN2, Bcl-2, Bax, pro-caspase-3 , cleaved caspase- 3, MMP-2 and MMP-9 in each group were detected by Western blot. Results T40 significantly inhibited U87 and U251 proliferation, clone formation, migration and invasion and promoted apoptosis ( P < 0. 05 ) ; T40 had 37 targets, among which the expression levels of PTPN1 and PTPN2 were negatively correlated with the overall survival rate of glioma patients; T40 signifi cantly reduced the expression of PTPN1, PTPN2, Bcl- 2, MMP-2 and MMP-9 in U87 and U251 cells, and increased the expression of Bax and cleaved caspase-3 (P < 0. 05). Conclusions T40 inhibits the proliferation , migration and invasion of glioma U87 and U251 cells and promotes their apoptosis, and its mechanism may be related to the reduction of PTPN1 and PTPN2 expression.
ABSTRACT
<p><b>OBJECTIVE</b>To observe effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in rat polarized osteoclasts.</p><p><b>METHODS</b>Rat polarized osteoclasts suffered 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 fluid shear stress for 30 min. mRNA expression of ATP6V1a1 was detected by Real-Time fluorescent quantitation PCR.</p><p><b>RESULTS</b>mRNA expression of ATP6V1a1 in 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 groups was (1.14 +/- 0.06) x 10(6), (1.62 +/- 0.09) x 10(6), (2.28 +/- 0.13) x 10(6), (3.24 +/- 0.18) x 10(6), (9.16 +/- 0.53) x 10(6) copy numbers, respectively (P < 0.05).</p><p><b>CONCLUSION</b>In the present study, polarized osteoclasts are sensitive to fluid shear stress. mRNA expression of ATP6V1a1 has increscent tendency along with increasing of fluid shear stress strength.</p>