ABSTRACT
Malignant tumors, whose occurrence and development are related to a variety of RNA transporter proteins, seriously affect human health and quality of life. Under normal circumstances, RNA transport proteins help RNA shuttle between nucleus and cytoplasm and their precise localization, effectively coupling the life activities in the nucleus and cytoplasm. During the process of tumorigenesis and progression, the expression and localization of some RNA transporters are abnormal or dysfunctional, which can change the subcellular localization, expression level, transport efficiency of downstream key RNA molecules, and the decay rate of cytoplasmic mRNA, and affect the proliferation, invasion and metastasis of tumors. This paper mainly reviews RNA transport proteins and their expression changes and regulation in tumors.
ABSTRACT
Malignant tumors, whose occurrence and development are related to a variety of RNA transporter proteins, seriously affect human health and quality of life. Under normal circumstances, RNA transport proteins help RNA shuttle between nucleus and cytoplasm and their precise localization, effectively coupling the life activities in the nucleus and cytoplasm. During the process of tumorigenesis and progression, the expression and localization of some RNA transporters are abnormal or dysfunctional, which can change the subcellular localization, expression level, transport efficiency of downstream key RNA molecules, and the decay rate of cytoplasmic mRNA, and affect the proliferation, invasion and metastasis of tumors. This paper mainly reviews RNA transport proteins and their expression changes and regulation in tumors.
ABSTRACT
<p><b>OBJECTIVE</b>To construct the recombinant plasmid pcDNA3.0-RGC32 and evaluate the effect of the response gene to complement-32 (RGC32) on cell cytoskeleton in vitro.</p><p><b>METHODS</b>The full-length cDNA of RGC32 was obtained by RT-PCR and inserted into the eukaryotic expression vector pcDNA3.0 to generate the recombinant plasmid pcDNA3.0-RGC32. After transfection of the recombinant plasmid into SW480 cells, the expression of RGC32 in the cells was detected by Western blotting. The cytoskeleton of SW480 cells was visualized before and after the transfection, and the changes in the cell migration ability was assessed by wound-healing assay.</p><p><b>RESULTS</b>The recombinant plasmid pcDNA3.0-RGC32 was successfully constructed. The expression of RGC32 was significantly increased in SW480 cells after transfection with pcDNA3.0-RGC32. Before the transfection, the microfilaments of SW480 cells were few and short without obvious polarity, but after the transfection, the microfilaments were increased and elongated with also an obvious polarity, and the invasive structures of lamellae and lamellipodia occurred. The migration ability of the cells was enhanced after transfection with pcDNA3.0-RGC32.</p><p><b>CONCLUSION</b>Overexpression of RGC32 can cause the reorganization of cytoskeleton and promotes the cell migration, which can be an important mechanism of RGC32 in promoting cancer metastasis.</p>
Subject(s)
Humans , Cell Cycle Proteins , Genetics , Cell Line, Tumor , Cell Movement , Colorectal Neoplasms , Genetics , Metabolism , Pathology , Cytoskeleton , Chemistry , Metabolism , Genetic Vectors , Muscle Proteins , Genetics , Neoplasm Metastasis , Genetics , Nerve Tissue Proteins , Genetics , Plasmids , Genetics , Recombinant Proteins , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To investigate the acute toxicity and assess the median lethal dose (LD50) of matrine in Kunming mice.</p><p><b>METHODS</b>Matrine at different doses were administered in Kunming mice via intraperitoneal injection, and the toxic reactions and LD50 of matrine was observed and determined.</p><p><b>RESULTS</b>The acute toxicity test of matrine indicated that the tolerable dose of matrine was above 80 mg/kg in Kunming mice, and the LD50 was 157.13 mg/kg (95%CI, 88.08-280.31 mg/kg). Morphological observation revealed degenerative changes of the nerve cells in the brain tissue of the mice.</p><p><b>CONCLUSION</b>The nervous system is the main target organ by the toxicity of matrine.</p>