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Objective: To study the chemical constituents of ethyl acetate extracts from the root bark of Celastrusmonospermus. Methods: The compounds were separated and purified by repeated column chromatographic methods including silica gel, ODS and Sephadex LH-20, and the chemical structures of compounds were determined by spectral data analyses. Results: Twelve compounds were obtained and identified as polpunonic acid (1), p-hydroxybenzoic acid (2), 4-hydroxy-3-methoxybenzoic acid (3), salaspermic acid (4), 3-oxo-2β-hydroxyfriedelan-29-oic acid (5), celastrol (6), 3α,22β-dihydroxy-olean-12-en-29-oic acid (7), orthosphenic acid (8), 21-oxo-2α,3α,22β-trihydroxy-29-nor-friedelan-24,2â-lactone (9), 2,3-dioxo-6α,10-dihydroxy-24-nor-friedelan-4,7-dien-29-oic acid (10), 2α,3β,19α,23-tetrahydroxyolean-12-en-28-oic acid (11) and 2α,3β-dihydroxyolean-12-en-23,28,30-trioic acid (12). Conclusion: Compounds 1, 3, 7, 10-12are obtained from this plant for the first time, and compounds 3, 11, 12 of them were separated from the genus Celastrus for the first time.
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OBJECTIVE: To study the chemical constituents from the root bark of Celastrus monospermus Roxb. METHODS: The compounds were isolate and purified by repeated column chromatography on silica gel, ODS and Sephadex LH-20, and the chemical structures of compounds were determined by spectral data analyses. RESULTS: Twenty-three compounds were isolated from this plant, which were identified as β-sitosterol palmitate (1), friedelan-3-one (2), 3β-hydroxyolean-12-en (3), 3β-hydroxylcholest-5,22-dien (4), β-sitosterol (5), 3,22-dioxo-20-taraxastene (6), 3,12-dioxofriedelane (7), 3-oxo-28-hydroxyfriedelane (8), 3-oxo-11β-hydroxyfriedelane (9), 3-oxo-12α-hydroxyfriedelane (10), pristimerin(11), 3-oxo-30-hydroxyfriedelane (12), 3-oxo-29-friedelanoic acid (13), salaspermic acid (14), celastrol (15), 3β, 21β-dihydroxy-30-nor-friedelan-20(29)-en-27-oic acid (16), 3β, 22α-dihydroxy-olean-12-en-29-oic acid (17), wilforol A (18), orthosphenic acid (19), p-hydroxybenzoic acid (20), 3-oxo-2β-hydroxyfriedelan-29-oic acid (21), 21-oxo-2α,3α,22β-trihydroxy-29-nor-friedelan-24,2β-lactone (22)and daucosterol (23). CONCLUSION: Compounds 1,6, 13-14,16-22 are obtained from this plant for the first time, and compounds 16, 20-22 are isolated from the genus Celastrus for the first time.
ABSTRACT
The chemical constituents from lipophilic parts of the stems of Celastrus monospermus were studied in this paper. The compounds were separated and purified by repeated column chromatographic methods including silica gel, ODS and Sephadex LH-20, and the structures of compounds were determined by spectral data analyses. Twenty six compounds were obtained and identified as 3-oxofriedelane(1), 3-oxofriedelan-28-al(2), 3,12-dioxofriedelane(3), 3β-hydroxyolean-12-en(4), 3-oxo-28-hydroxyfriedelane(5), 3-oxo-29-hydroxyfriedelane(6), 3-oxo-11β-hydroxyfriedel-ane(7), 3-oxo-16α-hydroxyfriedelane(8), 3,12-dioxo-28-hydroxyfriedelane(9), 1,3-dioxo-15α-hydroxyfriedelane(10), 3β,6α-dihydroxyolean-12-en(11), 3-oxo-7α,26-dihydroxyfriedel-ane(12), oleanolic acid(13), 3,15-dioxofriedelane(14), 3α-friedelinol(15), 3,12-dioxofriedelan-28-al(16), 3-oxo-12α-hydroxyfriedelane(17), 3,15-dioxo-12α-hydroxyfriedelane(18), 3β,11β-dihydroxyolean-12-en(19), 1β,3β-dihydroxylupan-20(29)-en(20), 3-oxo-12α,28-dihydroxyfriedelane(21), 3β,23-epoxyfriedelan-28-oic acid(22), salaquinone A(23), 2α,3β-dihydroxyfriedelan-28-oic acid(24), 23-nor-6-oxodemethylpristimerol(25) and 3-oxo-friedelan-27,28-dioic acid(26). Among them, compounds 8, 10-15, 18-20, 22-26 were obtained from this plant for the first time, and compounds 8, 10, 12, 14-15, 18, 22-24, 26 were separated from the genus Celastrus for the first time.
Subject(s)
Celastrus , Chemistry , Phytochemicals , Plant Stems , Chemistry , TriterpenesABSTRACT
OBJECTIVE: To study the effect of gold nanocage cage on the activation of reactive oxygen species (ROS) by photothermal inducing hydrogen peroxide (H2O2) in near infrared, and to analyze the relationship between ROS type and its yield and H2O2 concentration. To provide the experimental basis for the application of gold nanocage in the field of biomedicine and photothermal therapy. METHODS: The mixed solution of different concentrations of H2O2 with gold nanocage was irradiated by the equal power 808 nm near infrared laser, and the type and content of ROS produced in the different mixed solutions were detected by microprate reader and ROS fluorescent probe. RESULTS: Because of surface plasmon resonance (SPR), gold nanocage can not only produce overheating effect, but also induce H2O2 to generate more ROS (mainly single-phase oxygen and hydroxyl radicals, the content of them increases with the increase of H2O2 concentration) under the near infrared excitation. CONCLUSION: Gold nanocage can induce H2O2 to generate ROS such as single-phase oxygen and hydroxyl radicals under the near infrared excitation, and the relationship is positively correlated between the content of ROS and H2O2 concentration.
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<p><b>OBJECTIVE</b>To investigate the effect of N-desulfated heparin on tumor metastasis, tumor angiogenesis and basic fibroblast growth factor(bFGF) gene expression of orthotopically implanted human gastric carcinoma in NOD-SCID mice.</p><p><b>METHODS</b>Human gastric cancer SGC-7901 tissues were orthotopically implanted into the stomach of the NOD-SCID mice. Twenty mice were randomly divided into two groups which received either intravenous injection of 0.9% NaCl solution(0.9%NaCl solution group) or 10 mg/kg N-desulfated heparin (N-desulfated heparin group) twice a week for three weeks. Mice were sacrificed six weeks after tumor implantation. Tissues from stomach and other organs were obtained for histopathological evaluation. The intratumoral microvessel density (MVD) in tumor was evaluated immunohistochemically. Real time PCR was used to detect bFGF mRNA expression.</p><p><b>RESULTS</b>The tumor metastasis rates were 9/10 in 0.9% NaCl solution group and 2/10 in N-desulfated heparin group(P<0.05).MVD was 9.1+/-3.4 in 0.9% NaCl solution group and 4.7+/-1.8 in N-desulfated heparin group (t=3.617,P<0.05). bFGF mRNA expression was lower in N-desulfated heparin group(2.60+/-0.56%)than that in 0.9% NaCl solution group(30.65+/-6.84%).</p><p><b>CONCLUSION</b>N-desulfated heparin can inhibit the metastasis of gastric cancer through inhibiting tumor bFGF gene expression and tumor angiogenesis with no obvious anticoagulant activity.</p>