ABSTRACT
OBJECTIVE To explore the auditory outcomes and prognostic factors in ossiculoplasty using autogenous ossicles. METHODS A retrospective review was performed in 126 patients with cholesteatoma or chronic otitis media, who admitted to the Department of Otolaryngology in Jingzhou hospital affiliated to Tongji medical college from January 2014 to July 2016, and underwent canal wall-down tympanoplasty (CWDT) with ossiculoplasty using autogenous ossicles in a single stage. The postoperative complication and hearing thresholds were analyzed after 12 months' follow-up. RESULTS The rate of dry ear was 96.5％. We had not found any extrusion of prosthesis. There was no one with postoperative retraction pocket or recurrence of cholestatoma during the follow-up. Auditory outcomes showed air conduction threshold improved from (52.7±7.4)dB to (39.0±9.1)dB after operation, while the air-bone gaps improved from(27.4±6.9)dB to (20.8±6.2)dB. Postoperative outcomes were considered successful, if the postoperative air-bone gap was <20 dB. The successful hearing was achieved in 88 patients (69.8％). Prognostic factors were analyzed using multivariate analysis with logistic regression. And we found the presence of the stapes and the malleus handle was significantly favorable predictive factors. All the patients recovered well without severe complication. CONCLUSION Autogenous ossicles is very valuable in ossiculoplasty. The present stapes and malleus handle are important factors for the auditory outcomes in ossiculoplasty.
ABSTRACT
Objective To investigate the effect of continuous care provided by midwife group personnel to pregnant women.Methods 100 cases of pregnant women were screened out and divided into the study group and the control group with 50 patients in each group randomly.The study group received continuous care provided by midwife group personnel,the control group received traditional nursing.Effect of different nursing measures on mothers and neonates were compared.Results Antenatal cognition and rate of natural delivery of the study group were higher than the control group.The rate of neonatal asphyxia and postpartum depression occurred in the study group were lower than the control group,Maternal and family sarisfaction and rates of breasffeeding of the study group were higher than the control group.Conclusions Continuoas care provided by midwife group personnel can increase antenatal cognition and rate of natural delivery,maternal and family satisfaction.breast feeding rates and the overall quality of nursing staff,reduce rate of neonatal asphyxia with few postpartum depression,then improve the quality of perinatal care.
ABSTRACT
In order to construct a novel fusion protein PTD-maxadilan (PTD-MAX) that can enter the blood-brain barrier (BBB) efficiently, a new gene encoding PTD-MAX was synthesized and cloned into the expression vector pKYB. The recombinant vector pKYB-PTD-MAX was transformed into Escherichia coli ER2566. The expression of fusion protein consisting of PTD-MAX, intein and chitin binding domain was induced by IPTG and the target PTD-MAX protein was purified using Intein Mediated Purification with an Affinity Chitin-binding Tag system. The molecular weight of PTD-MAX determined by the laser flight mass spectrometry was coherent with its theoretical value. The results of the experiment in vivo indicated that the recombinant PTD-MAX can permeate into BBS and inhibitory effects on the food intake were more significantly than maxadilan (P<0.05). The preparation of PTD-MAX lay the foundation for its further application.