Effects of EBV infection on growth and apoptosis of nasopharyngeal carcinoma cell line / 中国病理生理杂志

AIM: To explore the effect of EBV infection on growth and apoptosis of nasopharyngeal carcinoma(NPC) cell line.METHODS: NPC cell line CNE1 was directly infected by Epstein Barr virus (EBV). The expression of EBV-latent membrane protein 1 (EBV-LMP1) and bcl-2 were detected by immunohistochemistry method (LSAB). The growth of NPC cells was identified by MTT method. Apoptotic carcinoma cells were detected by flow cytometry analysis and the terminal deoxynucletidyl transferase-medicated dUTP-biotin nick end labeling (TUNEL) methods. RESULTS: EBV-LMP1 was positive in CNE1 infected by EBV(E-CNE1). Compared with CEN1, the growth of E-CNE1 apparently increased ( P

The effect of EBV latent membrane protein 1 on proliferation and cell cycle of nasopharyngeal carcinoma cells / 中国病理生理杂志

AIM:To investigate the effect of Epstain-Barr virus latent membrane protein 1 (EBV-LMP1) on proliferation and cell cycle of nasopharyngeal carcinoma (NPC) cells. METHODS:The expression of EBV-LMP1 was detected by immunohistochemical method (LSAB). Proliferation of NPC cells was identified by MTT method. Cell cycle percentage was detected by flow cytometry analysis. RESULTS: OD value of EBV-LMP1 expressive NPC cells L-CEN1 was much higher than that of both EBV-LMP1 negative NPC cells V-CEN1 and CNE1 ( P 0.05).CONCLUSION:The expression of EBV-LMP1 on NPC cell might cause some change of cell cycle and enhance cell proliferation.

Response to some antitumor agents in epithelial cell line of poorly differentiated nasopharyngeal carcinoma (CNE-2z) / 中国病理生理杂志

Using the method to evaluate the cell survival rate in culture, the authors have studied the response of CNE-2z to six kinds of antitumor agents, nitrogen mustard, nitrocaphan, thiophosphoramide, fluorouracil, arabinsyl cytosine, vineristine. The cell survival rate relating to the given dose and lasting time of medicine is observed. Their relationships with different kinds of medicine have shown in different kinds of regression curve and experimental equations that are dependent on their pharmacological actions. The authors also have compared the responses of CNE-2z to each drug in the cell inhibition rate, the ratio of effective inhibiting concentration and therapeutic dosage, and the ratio of LD50 and effective inhibiting concentration. The results indicated that flurouracil may be more effective to nasopharyngeal carcinoma and of wilder safty range in human body.