ABSTRACT
OBJECTIVE:To provide theoretical basis for the detection of paracetamol by infrared spectroscopy. METHODS:0.002-0.02 g paracetamol was accurately weighed,added into 0.2 g potassium bromide,respectively,fully ground and well mixed, potassium bromide pressing plate method was used for to-be-tested test sample. Using hollow cathode lamp as light,scanning fre-quency was 30 times,resolution was 0.5 cm-1,scan range was 4000-400 cm-1,infrared absorption spectrum was determined and recorded to select the characteristic absorption peaks,then determine the optimized characteristic absorption peaks. Linear and non-linear models were respectively established by using mathematics modeling methods. RESULTS:1 016 cm-1 absorption peak with weaker absorbance but stronger features was selected for the analysis. According to the model establishing and calculation,the accu-racy of the nonlinear model was much higher than the linear model,r=0.942. CONCLUSIONS:Nonlinear quantitative model for quantitatively determining the content of paracetamol is feasible,and suitable for the on-destructive and rapid on-line quality control of paracetamol.
ABSTRACT
Objective To investigate ACEI ( benazepril ) and tranilast exert renoprotective properties in diabetic nephropathy( DN) through the inhibition of thioredoxin( Trx) . Methods Forty male SD rats were randomly divided into normal control group,model control group,tranilast group and benazepril group (n=10 each).Normal control group was fed with normal diet. Other groups were fed with high-glucose high-fat diet to make DN models. Rats in model control, tranilast, and benazepril groups were fed with normal diet,400 mg??kg-1??d-1 tranilast plus normal diet,and 10 mg??kg-1??d-1 benazepril plus normal diet,respectively,via oral gavage for 12 weeks.The 24-hour proteinuria,blood glucose(BG),blood urea nitrogen (BUN),serum creatinine ( Scr) and renal pathology changes were detected. Expression of Trx was measured by Western-blot. Results The 24 h urine protein, BG, BUN, Scr, kidney/body weight, and glomerular sclerosis index were significantly decreased in tranilast group and benazepril group,as compaired with model control group ( P0.05).Both tranilast and benazepril can reduce renal pathological changes,and can increase the expression of Trx of DN rats, but benazepril had a more significant effect on increasing Trx expression. Conclusion Both tranilast and benazepril have renoprotective function in DN, and benazepril is more effective than tranilast in delaying the progression of diabetic nephropathy by increasing Trx expression and decreasomg oxidative stress.