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Indian J Biochem Biophys ; 1991 Oct-Dec; 28(5-6): 472-5
Article in English | IMSEAR | ID: sea-28380

ABSTRACT

Transposon, Tn917, carried on pTV1 plasmid has been used successfully to mutagenise Bacillus brevis. The transposon showed preference for insertion at an "aro" site. A second insertional event after elimination of the preferred site with ethidium bromide/acridine orange treatment has permitted isolation of Gln- mutants in B. brevis.


Subject(s)
Bacillus/genetics , DNA Transposable Elements , Glutamine/genetics , Mutagenesis, Insertional
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