Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add filters








Language
Year range
1.
Egyptian Journal of Hospital Medicine [The]. 2005; 19 (June): 138-155
in English | IMEMR | ID: emr-200659

ABSTRACT

This study was conducted to compare between the possible effects of rosiglitazone "A new oral antidiabetic drug with selective PPAR-gamma agonistic effect" in a dose of 0.03 mg/kg BW and gliclazide " An oral antidiabetic sulphonylurea" in a dose of 10 mg/kg BW either used alone or in combination, for 6 weeks on the liver, serum glucose and lipid profile in streptozotocin diabetic rats


Thirty rats were randomized into 5 groups [n=6]. Group I; the control group was given saline orally daily for 6 weeks. Group II; the streptozotocin induced diabetic group. Group III received rosiglitazone, while group IV received gliclazide and group V received both drugs


The results of the present study revealed that streptozotocin significantly [P< 0.05] elevated serum glucose, cholesterol and triglycerides in rats compared to the controls. The insulin sensitizer "rosiglitazone" either alone or combined with gliclazide decreased serum glucose significantly [P< 0.05] compared to the diabetic group. Gliclazide alone also had the same effect. Rosiglitazone alone decreased serum cholesterol and AST and in combination with gliclazide decreased serum ALT significantly [P< 0.05] compared to the diabetic group


For histopathological study, liver tissue was prepared for both histological [HandE, PAS and Masson's trichrome] and immunohistochemical [alpha 1 antitrypsin expression] techniques. Both qualitative and quantitative analysis was done to assess the degree of hepatic damage. According to certain criteria, HandE stained sections were quantitatively examined to assess the degree of hepatocyte affection, beside other quantitative measurements [optical density and color area percentage] using the image analyser. Obtained results revealed that streptozotocin caused severe affection in 6% of hepatocytes, mild affection in 2% and moderate affection in 41%. The drug also resulted in significant increase in PAS stained glycogen granules in hepatocytes as well as collagen in portal tracts. Immunostaining of alpha 1 antitrypsin revealed increased expression in the lining of blood sinusoids including Kupffer cell cytoplasm and in the area around the central vein. Groups III, IV and V which were under the effect of rosiglitazone, gliclazide or both respectively, showed hepatocyte damage similar to that of diabetic control group; however the degree of that damage was only statistically significantly increased in case of group III


When compared to diabetic control group, these groups [III, IV and V] showed no significant difference in both optical density of PAS positive reaction or mean color area percentage of collagen; however the mean optical density of immunostaining decreased significantly


This indicated that rosiglitazone alone or when used concomitantly with gliclazide, in streptozotocin-induced diabetic rats resulted in improvement of their metabolic control, yet the potential of hepatotoxicity was still to be considered

SELECTION OF CITATIONS
SEARCH DETAIL