Forced degradation of gliquidone and development of validated stability-indicating HPLC and TLC methods

Forced degradation studies of gliquidone were conducted under different stress conditions. Three degradates were observed upon using HPLC and TLC and elucidated by LC-MS and IR. HPLC method was performed on C18 column using methanol-water (85:15 v/v) pH 3.5 as a mobile phase with isocratic mode at 1 mL.min-1 and detection at 225 nm. HPLC analysis was applied in range of 0.5-20 µg.mL-1 (r =1) with limit of detection (LOD) 0.177 µg.mL-1. TLC method was based on the separation of gliquidone from degradation products on silica gel TLC F254 plates using chloroform-cyclohexane-glacial acetic acid (6:3:1v/v) as a developing system with relative retardation 1.15±0.01. Densitometric measurements were achieved in range of 2 -20 µg /band at 254 nm (r = 0.9999) with LOD of 0.26 µg /band. Least squares regression analysis was applied to provide mathematical estimates of the degree of linearity. The analysis revealed a linear calibration for HPLC where a binomial relationship for TLC. Stability testing and methods validation have been evaluated according to International Conference on Harmonization guidelines. Moreover, the proposed methods were applied for the analysis of tablets and the results obtained were statistically compared with those of pharmacopeial method revealing no significant difference about accuracy and precision.

Clinico-diagnostic study on bovine viral diarrhea [BYD] in sheep

A private farm at Giza govemorate of three hundred sheep was subjected for virological, immuno florescent, immuno cytochemical and histopathological examinations. In this farm three sheep were dead [1% mortality] and forty animals [13% morbidity] showed diarrhea, fever [3 9°C to 40°C] and muco to muco- purulent nasal discharges associated with mild cough. This study was triggered by isolation of the causative virus from nasal swabs of sick animals and tissues of dead animals on Madin Darby Bovine Kidney [MDBK] cells. The causative virus was identified as Bovine Virus Diarrhea [BVD] by usingindirect immuno fluorescent antibody technique [IFAT] followed by immuno cytochemical identification by using cell culture irnmuno peroxides test. Dead animals subjected to autopsy and histopathological examination of different organs and lymph nodes. Marked involvement of the lymphoid tissues particularly the mesenteric lymph nodes and peyeres patches of the intestinal ileum were prominent on histopathological examination, in addition to necrosis of the tips of the intestinal villi of the ileum and necrosis of the intestinal gland. This study concluded that infection with BVDV should be considered as one of infectious causes of enteritis

Immunological Role of Nasal Staphylococcus aureus Carriage in Patients with Persistent at Allergic Rhinitis

Nasal carriage of staphylococcus aureus (S.aureus) exerts immunomodulatory effect in patients with atopic dermatitis and it may contribute to airway inflammation and allergic response in patients with allergic rhinitis. We Aim to investigate the frequency of nasal S.aureus carriage in patients with persistent allergic rhinitis and its possible influence on their symptoms and immune markers. We chosed 20 non smoker patients with house dust mite (HDM) allergy causing allergic rhinitis and 20 non smoker healthy subjects matched for age and sex. For all subjects rhinoscopy was done; skin prick test; nasal culture for S.aureus; nasal interleukin 4;nasal total IgE; serum total IgE and serum specific IgE(SSIgE) for HDM. Nasal S.aureus was detected in 16/20 patients (80) and 5/20 (25) in healthy subjects with highly significant statistical difference p0.01. Correlation of nasal staph.aureus count and different systemic and local immune markers revealed highly significant positive correlation between nasal S.aureus count and serum total IgE (r

Immunological Role of Nasal Staphylococcus aureus Carriage in Patients with Persistent at Allergic Rhinitis

Nasal carriage of staphylococcus aureus (S.aureus) exerts immunomodulatory effect in patients with atopic dermatitis and it may contribute to airway inflammation and allergic response in patients with allergic rhinitis. We Aim to investigate the frequency of nasal S.aureus carriage in patients with persistent allergic rhinitis and its possible influence on their symptoms and immune markers. We chosed 20 non smoker patients with house dust mite (HDM) allergy causing allergic rhinitis and 20 non smoker healthy subjects matched for age and sex. For all subjects rhinoscopy was done; skin prick test; nasal culture for S.aureus; nasal interleukin 4;nasal total IgE; serum total IgE and serum specific IgE(SSIgE) for HDM. Nasal S.aureus was detected in 16/20 patients (80) and 5/20 (25) in healthy subjects with highly significant statistical difference p0.01. Correlation of nasal staph.aureus count and different systemic and local immune markers revealed highly significant positive correlation between nasal S.aureus count and serum total IgE (r

Triphasic helical CT of hepatocellular carcinoma

Hepatocellular carcinoma [HCC] is the most frequent primary malignancy of the Iiver. Helical CT is highly suitable for imaging of HCC. To identify tumor localization, extent and vascular pattern at different phases of contrast enhanced helical CT. Our study included 25 patients. The age range was between 40 up to 65 years [average 52.5 y.]. The sex distribution was 17 male [17/25=68%] and 8 female cases [8/25=32%], they were examined firstly with abdominal US followed by triphasic helical abdominal CT. CT guided biopsy was done for all cases. The lobar hepatic distribution of HCC was 18 cases within the RT. Rt. Lobe [18/25=72%], 4 cases within the lt. lobe [4/25=16%], and 3 cases involving both lobes [3/25=12%]. Solitary lesions were seen in 19 cases [19/25=76%] and multifocal type in 6 cases [6/25=24%]. HCC was large [more than 3 cm] in 13 cases [13/25=52%] and small in size [less than] 3 cm] in 12 cases [12/25=48%]. The pre-contrast CT density pattern of HCC were of mixed density in 10 cases, hypodense in 13 cases, hyperdense in one case and isodensc in one case. Post-contrast CT density pattern in the asterisk arterial phase were 10 cases, mixed density, 4 cases marginal enhancement, 3 cases hyperdense, 2 cases mosaic and 2 cases nodular pattern. Asterisk asterisk Venous phase were 16 cases hypodensc, 5 cases isodense and 4 cases mixed density.21 cases [84%] were hypervascular and 4 cases [16%] were hypovascular. Tumor rupture was noted in two cases. Triphasic study of the liver is helpful in tumor localization. extension and size. In addition, it is useful in differentiation of vascular from non vascular tumors as well as identification of the different vascular patterns. Tumor rupture was easily diagnosed by helical CT

Early results of taivt trial

During the last decade few studies have been accomplished in the use of catheter directed thrombolysis for iliofemoral deep venous thrombosis to remove the thrombus completely and rapidly. These studies have shown that early thrombolysis preserve valve function and reduce the incidence of post phlebitic syndrome. However, no large randomised study has addressed its benefits, compared with those of anticoagulation. The aim of our study is to do a prospective randomised study comparing local Thrombolysis and Anticoagulant in Iliofemoral Venous Thrombosis [TAIVT trial] as regard venous function. Consecutive series of patients admitted to Suez Canal University hospital with ileofemoral DVT and comply with the inclusion criteria. Patients were randomised for either catheter directed thrombolysis using streptokinase as thrombolytic agent followed by anticoagulant or anticoagulant alone. Follow up of both arms of the trial will be at 6 weeks then 3 and 6 months post-treatment. At each follow up appointment, assessment of the venous function using colour duplex, air plethysmography and photoplethysmography.We had complete data from only 35 patients, 17 had anticoagulant and 18 had thrombolysis. At 6 months, patency rate was better in cases treated with thrombolysis, complete patency [13patients [72%] versus 2[12%], p < 0.001] and non patency [0 versus 7 [41%], p < 0.001]. Venous reflux was higher in-patients treated with anticoagulant [7 patients [41%] versus 2 [11%], p < 0.042]. Early results of the trial showed that patients treated with catheter directed thrombolysis have better venous function those with anticoagulant. This paper was presented at the XV European Surgical Vascular Society ESVS Conference. Lucerne Switzerland- September 2001